Secondary Antibodies

Human IgE mouse monoclonal antibody, clone MH25/1, Purified

Applications Immunofluorescence.
Immunohistochemistry on frozen and paraffin sections: 1/5 – 1/10 (Based on the Indirect method, with a normal ABC method the dilution can be 5-10 times higher, but the optimal dilution should be tested by serial dilution.)
Dilute immediately before use with PBS or TRIS.
Localisation: D ε 1 epitope on Fc Fragment
Positive control: Polypus nose
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone B572M, Purified

Applications ELISA.
Lateral flow assays.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc specific) mouse monoclonal antibody, clone MK1A6, Purified

Applications ELISA assay development of total IgG.
Passive hemagglutination assays.
Histological staining of Acetone Fixed Frozen tissue sections.
Reactivities Human
Conjugation Unconjugated

Human IgG (Fc CH2 Domain specific) mouse monoclonal antibody, clone 8A4, Purified

Applications ELISA.
Flow cytometry (1:50/1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Human
Conjugation Unconjugated

Human IgA2 mouse monoclonal antibody, clone A9604D2, Biotin

Applications ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation Biotin

Human IgA2 mouse monoclonal antibody, clone A9604D2, HRP

Applications ELISA: 1/500-1/2000.
Reactivities Human
Conjugation HRP

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, Biotin

Applications ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation Biotin

Human IgE mouse monoclonal antibody, clone 090-11348, Purified

Applications ELISA.
Recommended antibody pair for Sandwich Immunoassay:
Capture / Detection
AM00919PU-N / AM00918PU-N
Reactivities Human
Conjugation Unconjugated

Human IgA (secretory) mouse monoclonal antibody, clone B781M, Purified

Applications ELISA: 1/20,000 dilution will yield > 1.0 O.D. using Alkaline Phosphatase conjugated Rabbit anti-Mouse Ig.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone 090-11368, Purified

Applications ELISA.
Recommended pair for Sandwich Immunoassay
Capture: AM00919PU-N
Detection: AM00917PU-N
Reactivities Human
Conjugation Unconjugated

Human IgM rat monoclonal antibody, clone HM-7, Aff - Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone 47.2.E9, Purified

Applications ELISA (1/5000-1/10000)
Reactivities Human
Conjugation Unconjugated

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, Purified

Applications ELISA: 1/500-1/1000.
Reactivities Human
Conjugation Unconjugated

Human IgA mouse monoclonal antibody, clone DA43D3, Purified

Applications Suitable for Immunohistochemistry on Frozen (1 µg/ml, 1/400) and Paraffin Embedded Sections. Proteinase K pretreatment for antigen retrieval is recommended.
Suggested Positive Control: Human tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone DM3A3, Purified

Applications Immunohistochemistry on frozen sections: 1-2 µg/ml (1:200 - 1:400).
Immunohistochemistry on paraffin sections: Proteinase K pretreatment for antigen retrieval is recommended.
Suggested positive control: Human tonsil.
Reactivities Human
Conjugation Unconjugated