Mouse lambda light chain rabbit polyclonal antibody, HRP
| Applications | ELISA: 1/10,000 - 1/50,000 . Western Blot: 1/1,000 - 1/5,000. Immunohistochemistry on paraffin sections: 1/500 - 1/2,500. |
| Reactivities | Mouse |
| Conjugation | HRP |
Secondary Antibodies
Rabbit IgA+IgG+IgM (H+L chain) goat polyclonal antibody, TRITC
| Applications | Can be used to identify and measure immunoglobulins, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates. If two immunoconjugates with different labels but originating from the same host animal species are used for direct two-color staining, they can safely be mixed and used in a single step, without the danger of interaction between the two reagents. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended working dilutions: 1/20 - 1/80. |
| Reactivities | Rabbit |
| Conjugation | TRITC |
Monkey IgG (Fab specific) rabbit polyclonal antibody, TRITC
| Applications | Can be used for direct staining of fixed cell and tissue substrates; to demonstrate the intracellular presence of free or Ig-bound subunits of both kappa or lambda type. In general this conjugate is not recommended as direct or indirect screening reagent for If isotypes on surface membranes of vital lymphoid cells. The activity to the common Ig/Fab subunit may result in the staining of immunoglobulins bound to the Fc-receptors on non-lymphoid cells. Combinations of isotype-specific reagents should be used instead for this purpose. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:10 and 1:40. |
| Reactivities | Monkey |
| Conjugation | TRITC |
Human IgG rabbit polyclonal antibody, Purified
| Applications | Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use at 1/200-1/500 dilution in an ABC method (30 minutes at room temperature). Proteolytic treatment is required prior to immunostaining of formalin fixed paraffin embedded tissue sections. Recommended Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Rat IgG2a goat polyclonal antibody, HRP
| Applications | ELISA: 1/10,000. Western Blot: 1/1,000-1/10,000. |
| Reactivities | Rat |
| Conjugation | HRP |
Porcine IgA goat polyclonal antibody, HRP
| Applications | ELISA: 1/10,000-1/100,000. |
| Reactivities | Porcine |
| Conjugation | HRP |
Human IgG (F(ab)2 specific) goat polyclonal antibody, FITC
| Applications | Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of free of Ig bound subunits of both kappa and lambda type. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected. Recommended Dilutions: 1/20-1/80. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
| Reactivities | Human |
| Conjugation | FITC |
Rabbit IgG (H+L chain) goat polyclonal antibody, Texas Red, Lyophilized
| Applications | Suitable for immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. Recommended Dilutions FLISA: 1/10,000-1/50,000. Flow Cytometry: 1/500-1/2,500. IF Microscopy: 1/1000 - 1/5000. |
| Reactivities | Rabbit |
| Conjugation | Texas Red |
Mouse IgG (Fc specific) goat polyclonal antibody, Biotin
| Applications | ELISA: 1/20,000-1/100,000 Western blot: 1/2,000-1/10,000 Immunohistochemistry: 1/1,000-1/5,000. Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:40,000 to 1:130,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgG (Rat adsorbed) goat polyclonal antibody, Aff - Purified
| Applications | ELISA. Flow Cytometry. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (1/2,000-1/20,000 for Western blot), ELISA (1/180,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG (Fc specific) goat polyclonal antibody, Ig Fraction
| Applications | ELISA: 1/20000-1/100000. Western Blot: 1/2000-1/10000. Immunohistochemistry: 1/1000-1/5,000. |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG goat polyclonal antibody, AP, Aff - Purified
| Applications | ELISA: 1/10000. Western Blot: 1/5000-1/10000, Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | AP |
Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, AP
| Applications | ELISA: 1/2,000-1/4,000. Immunoblotting. Immunohistochemistry. |
| Reactivities | Mouse |
| Conjugation | AP |
Mouse IgA goat polyclonal antibody, HRP
| Applications | Immunohistochemistry on frozen and paraffin sections. ELISA: 1/4000 - 1/8000. Western Blot. |
| Reactivities | Mouse |
| Conjugation | HRP |
