Secondary Antibodies

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, Purified

Applications Flow Cytometry: Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Purified

Applications Flow Cytometry: Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone SB73a, Purified

Applications Flow Cytometry: < / 1 µg/10e6 cells
Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells .
ELISA: 1 µg/ml.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, Biotin

Applications Flow Cytometry: Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation Biotin

Mouse IgE (Epsilon heavy chain) rat monoclonal antibody, clone 23G3, Biotin

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgE+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgE+ cells .
ELISA: 1/5000-1/10000.
Enzyme-Linked-Immunosorbent-Assay.
Reactivities Mouse
Conjugation Biotin

Human IgE mouse monoclonal antibody, clone 4H10, Purified

Applications Flow Cytometry: 4 µg/ml.
ELISA.
Western Blot.
Reactivities Human
Conjugation Unconjugated

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Biotin

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/100 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

F(ab')2 Rat IgG F(ab')2 Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:5,000 - 1:200,000
IHC: 1:500 - 1:5,000
ELISA: 1:5,000 - 1:100,000
Reactivities Rat
Conjugation HRP

F(ab')2 Rat IgG (H&L) Antibody Peroxidase Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:500 - 1:2,500
ELISA: 1:100,000
Reactivities Rat
Conjugation HRP

F(ab')2 Rat IgG (H&L) Antibody

Applications WB: 1:2,000-1:10,000
IHC: 1:1,000-1:5,000
ELISA: 1:20,000-1:100,000
Conjugation Unconjugated

F(ab')2 Rat IgG (H&L) Antibody Alkaline Phosphatase Conjugated Pre-Adsorbed

Applications WB: 1:500 - 1:2,500
IHC: 1:200 - 1:1,000
ELISA: 1:2,000 - 1:10,000
Conjugation Alkaline Phosphatase

F(ab')2 Rat IgG (H&L) Antibody Biotin Conjugated Pre-Adsorbed

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Biotin

F(ab')2 Guinea Pig IgG (H&L) Antibody

Applications WB: 1:2,000-1:10,000
IHC: 1:1,000-1:5,000
ELISA: 1:20,000-1:100,000
Conjugation Unconjugated

F(ab')2 Guinea Pig IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Applications WB: 1:1,000-1:5,000
IHC: 1:500-1:1,000
ELISA: 1:10,000 - 1:50,000
Conjugation Alkaline Phosphatase