Secondary Antibodies

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, FITC

Applications FLISA:1:200-1:400
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Goat IgG (Fc specific) mouse monoclonal antibody, clone SB115d, HRP

Applications ELISA: 1/10,000-1/20,000.
Reactivities Goat
Conjugation HRP

Mouse IgD (Delta heavy chain) rat monoclonal antibody, clone 11-26, Biotin

Applications Flow Cytometry: < / = 1µg/10e6 cells. (See Ref.1-5)
Reactivities Mouse
Conjugation Biotin

Human IgG (gamma chain specific) mouse monoclonal antibody, clone JDC-10, PE

Applications Indirect Immunofluorescent staining of IgG+ human B lymphocytes: < / = 0.2 µg/10e6 cells.
Reactivities Human
Conjugation PE

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, FITC

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Rabbit IgG mouse monoclonal antibody, clone 2A9, HRP

Applications ELISA: 1/4,000-1/8,000.
Reactivities Rabbit
Conjugation HRP

Human IgG2 (Fc specific) mouse monoclonal antibody, clone 31-7-4, AP

Applications ELISA: 1/500-1/1,000.
Reactivities Human
Conjugation AP

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, FITC

Applications FLISA: 1/200-1/600.
Indirect Immunofluorescent staining
of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
Reactivities Human
Conjugation FITC

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, HRP

Applications ELISA: 1/4,000-1/8,000 (See also Ref.1).
Reactivities Human
Conjugation HRP

Human IgM mouse monoclonal antibody, clone R1/69, Supernatant

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature).
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated

Human IgG (heavy chain) mouse monoclonal antibody, clone B33/20, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Monkey IgG (gamma chain specific) goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
FLISA: 1:10,000-1:50,000
Immunofluorescence: 1:1,000-1:5,000.
Reactivities Monkey
Conjugation FITC