Secondary Antibodies

Mouse IgG (H+L chain) chicken polyclonal antibody, FITC

Applications Immunocytochemistry.
Immunohistochemistry (1/250).
Quality Control: Antibodies were tested for specificity using Double-Immunodiffusion assays in agarose and for non-specific binding using Cryostat sections of unfixed embryonic chick tissues.
Reactivities Mouse
Conjugation FITC

Mouse IgG rabbit polyclonal antibody, Serum

Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (H+L chain) rabbit polyclonal antibody, Ig Fraction

Applications Immunoprecipitation.
Immunodiffusion.

Ouchterlony Test:
Sample size: 15 µl.
Distance between wells: 5 mm.
Test Time: 5 days.
Temperature: 37°C.
Samples Tested: Normal mouse Serum (NMS), Purified mouse IgG, Normal human serum (NHS), Purified human IgG.
Results:
Specificity: a single band against NMS was detected at dilutions of 1/4 and 1/8 which is homogeneous with band to purified mouse IgG.
No bands were detected against human IgG at 5 mg/ml or NHS at 1/4 dilution.
Protein content: Biuret Assay 14 mg/ml.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG (adsorbed) goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Western Blot: 1/1000-1/10000.
Reactivities Mouse
Conjugation HRP

Mouse IgG (adsorbed) goat polyclonal antibody, AP

Applications ELISA: 1/200-1/2500.
Western Blot: 1/100-1/500.
Reactivities Mouse
Conjugation AP

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on paraffin sections.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, Biotin, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections.
Reactivities Mouse
Conjugation Biotin

Mouse IgA (heavy chain) rat monoclonal antibody, clone LO-MA-7, HRP, Purified

Applications Immunoassay.
ELISA: good binding on plastic.
Immunohistochemistry on frozen sections.
Reactivities Mouse
Conjugation HRP

Mouse IgG2a (heavy chain) rat monoclonal antibody, clone LO-MG2a-2, Aff - Purified

Applications Immunoassay.
Immunohistochemistry on Frozen Sections.
The specificity of monoclonal antibody anti-IgG subclasses is determined in a range of optimal concentrations. Increasing of the first or second antibody at a high level can induce cross-reactions with other IgG subclasses.
Reactivities Mouse
Conjugation Unconjugated

Mouse IgG2a (gamma 2a chain specific) rat monoclonal antibody, clone SB84a, PE

Applications Flow Cytometry: ≤ 0.2 µg/106 cells.
Identification and enumeration of IgG2a+.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+.
ELISA/FLISA.
Also used as a second step reagent for mouse IgG2a monoclonal antibodies.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgA (alpha chain specific) rat monoclonal antibody, clone 11-44-2, PE

Applications Flow Cytometry: < / = 0.2 µg/10e6 cells.
Identification and enumeration of IgA+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgA+ cells.
Reactivities Mouse
Conjugation PE

Mouse IgM (Mu chain specific) rat monoclonal antibody, clone 1B4B1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Identification and enumeration of IgM+ cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
Also used as a second step reagent for mouse IgM monoclonal antibodies.
Reactivities Mouse
Conjugation FITC