Secondary Antibodies
Rabbit IgG (H+L chain, adsorbed) goat polyclonal antibody, Aff - Purified
| Applications | Suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user. ELISA: 1/15,000-1/30,000 Western blot: 1/500-1/2,000 Immunohistochemistry: 1/500-1/2,000. |
| Reactivities | Rabbit |
| Conjugation | Unconjugated |
Canine IgE goat polyclonal antibody, HRP
| Applications | ELISA: 1/10,000-1/100,000. Western Blot: 1/1000-1/10,000. Immunohistochemistry on Frozen Sections: 1/200-1/500. |
| Reactivities | Canine |
| Conjugation | HRP |
Canine IgG1 goat polyclonal antibody, Purified
| Applications | ELISA: 1:100 - 1:10,000. Western Blot: 1:1000 - 1:10,000. Immunohistochemistry on frozen sections: 1:200 - 1:2000. |
| Reactivities | Canine |
| Conjugation | Unconjugated |
Human IgG (Fc specific) goat polyclonal antibody, Aff - Purified
| Applications | ELISA: 1-5 µg/ml. Immunohistochemistry on Frozen Sections. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Rat IgG2ab (subclass specific) goat polyclonal antibody, TRITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgG2 in rat serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions 1/20 - 1/80, depending on the method used. |
| Reactivities | Rat |
| Conjugation | TRITC |
Rat IgG2ab (subclass specific) goat polyclonal antibody, FITC
| Applications | Can be used to identify and measure IgG2, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of rat origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions: 1/20 - 1/80. |
| Reactivities | Rat |
| Conjugation | FITC |
Rat IgG2ab (subclass specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection of IgG2a at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Recommended working dilutions: Histochemistry: 1/100 - 1/250. ELISA and comparable non-precipitating antibody-binding assays: 1/500 - 1/5000. |
| Reactivities | Rat |
| Conjugation | Unconjugated |
Rat IgG2ab (subclass specific) goat polyclonal antibody, Biotin
| Applications | Can be used: In immunocytochemical and Immunohistochemical staining for the detection of IgG2 at the cellular and subcellular level of appropriately treated cell and tissue substrates. To demonstrate circulating IgG2 antibodies in serodiagnostic microbiology and autoimmune diseases. To identify a specific antigen using a reference antibody of Rat origin known to be of the IgG2 isotype in the middle layer of the indirect test procedure. In non-isotopic assay methodology (e.g. ELISA) to measure IgG2 in rat serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. This immunoconjugate is not pre-diluted. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Recommended Working Dilutions: Histochemical and Cytochemical Use: 1/100-1/250. ELISA and comparable non-precipitating antibody-binding assays between: 1/2.000-1/10,000. |
| Reactivities | Rat |
| Conjugation | Biotin |
Mouse IgG3 (subclass specific) goat polyclonal antibody, TRITC
| Applications | Can be used in Immunocytochemical and Immunohistochemical staining for the detection of IgG3 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to identify and measure IgG3 in mouse serum or other body fluids. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions 1/10 - 1/40, depending on the method used. |
| Reactivities | Mouse |
| Conjugation | TRITC |
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Reactivities
- Mouse (396)
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- Rabbit (136)
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- Golden Syrian Hamster (7)
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- Mouse IgG (3)
- Human IgG (2)
- Reptiles (2)
- Goat IgG (1)
- Mammalian (1)
- Rabbit IgG (1)
