Secondary Antibodies

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Sheep IgG (Fc specific) Rabbit Polyclonal Antibody
AP33081AF-N

CNY 6580.00

5周

Sheep IgG (Fc specific) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.
Can be used As unlabelled primary or secondary reagent for indirect detection of IgG at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Recommended Working Dilutions:
Histochemistry Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5.000.
Antibody titre: 1/64 when tested against pooled normal sheep serum in agar-block immunodiffusion titration.
Reactivities Sheep
Conjugation Unconjugated
Duck IgG (H+L chain) Rabbit Polyclonal Antibody
AP33057AF-N

CNY 3402.00

5周

Duck IgG (H+L chain) rabbit polyclonal antibody, Azide Free

Applications ELISA.
Dot blot.
Immunoblotting.
Indirect Immunofluorescence.
Can be used as unlabelled primary or secondary reagent for indirect detection techniques, to prepare conjugates with markers of the user’s own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching or detection antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration.
Recommended Working Dilutions:
Histochemistry: 1/100-1/500.
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Antibody titre: Precipitin titre not less than 1/128 when tested against normal Duck serum in agar block titration.
Reactivities Duck
Conjugation Unconjugated
Duck IgG (H+L chain) Rabbit Polyclonal Antibody
AP33057FC-N

CNY 6730.00

5周

Duck IgG (H+L chain) rabbit polyclonal antibody, FITC

Applications ELISA.
Immunocytochemistry.
(In)direct Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Can be used to identify and measure IgG, antigen or antibody, at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen or immune complex using a reference antibody of duck origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions: 1/20-1/80.
Reactivities Duck
Conjugation FITC
Rat IgG2c Rabbit Polyclonal Antibody
AP05381PU-N

CNY 3200.00

4周

Rat IgG2c rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Human, Rat
Conjugation Unconjugated
Rat IgG2b Rabbit Polyclonal Antibody
AP05380PU-N

CNY 3200.00

4周

Rat IgG2b rabbit polyclonal antibody, Purified

Applications ELISA: 1µg/ml - 10µg/ml.
Western Blot.
Flow Cytometry.
Reactivities Rat
Conjugation Unconjugated
F(ab')2 Rat IgG (H&L) Antibody
TA398225

CNY 3019.00

4周

F(ab')2 Rat IgG (H&L) Antibody

Applications WB: 1:2,000-1:10,000
IHC: 1:1,000-1:5,000
ELISA: 1:20,000-1:100,000
Conjugation Unconjugated
F(ab')2 Guinea Pig IgG (H&L) Antibody
TA398142

CNY 3131.00

4周

F(ab')2 Guinea Pig IgG (H&L) Antibody

Applications WB: 1:2,000-1:10,000
IHC: 1:1,000-1:5,000
ELISA: 1:20,000-1:100,000
Conjugation Unconjugated
F(ab')2 Guinea Pig IgG (H&L) Antibody Alkaline Phosphatase Conjugated
TA398145

CNY 4088.00

4周

F(ab')2 Guinea Pig IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Applications WB: 1:1,000-1:5,000
IHC: 1:500-1:1,000
ELISA: 1:10,000 - 1:50,000
Conjugation Alkaline Phosphatase
F(ab')2 Rat IgG (H&L) Antibody Biotin Conjugated
TA398229

CNY 2850.00

4周

F(ab')2 Rat IgG (H&L) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:250,000
Conjugation Biotin
F(ab')2 Horse IgG (H&L) Antibody Peroxidase Conjugated
TA398153

CNY 3938.00

4周

F(ab')2 Horse IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:1,000 - 1:5,000
IHC: 1:500 - 1:2,500
ELISA: 1:90,000
Conjugation HRP
F(ab')2 Horse IgG (H&L) Antibody Biotin Conjugated
TA398155

CNY 2963.00

4周

F(ab')2 Horse IgG (H&L) Antibody Biotin Conjugated

Applications WB: 1:10,000 - 1:25,000
IHC: 1:1,000 - 1:2,500
ELISA: 1:300,000
Conjugation Biotin
F(ab')2 Horse IgG (H&L) Antibody
TA398151

CNY 3000.00

4周

F(ab')2 Horse IgG (H&L) Antibody

Applications WB: 1:2,000-1:10,000
IHC: 1:1,000-1:5,000
ELISA: 1:20,000 - 1:100,000
Conjugation Unconjugated
F(ab')2 Guinea Pig IgG (H&L) Antibody Biotin Conjugated
TA398146

CNY 2963.00

4周

F(ab')2 Guinea Pig IgG (H&L) Antibody Biotin Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:90,000
Conjugation Biotin
F(ab')2 Horse IgG (H&L) Antibody Alkaline Phosphatase Conjugated
TA398154

CNY 4088.00

4周

F(ab')2 Horse IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Applications WB: 1:500-1:1,000
IHC: 1:250-1:500
ELISA: 1:1,000 - 1:15,000
Conjugation Alkaline Phosphatase
Bovine IgG (Fc specific) Rabbit Polyclonal Antibody
R1305HRP

CNY 3938.00

4周

Bovine IgG (Fc specific) rabbit polyclonal antibody, HRP

Applications Suitable for immunoblotting (western or dot blot 1:1,000 - 1:10,000), ELISA (1:10,000 - 1:50,000=, immunoperoxidase electron microscopy and immunohistochemistry (1:500 - 1;2,500) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency.
This product has been assayed against 1.0 ug of Bovine IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.
A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.
Reactivities Bovine
Conjugation HRP