Secondary Antibodies

Goat IgG (H+L chain) mouse polyclonal antibody, Serum

Applications ELISA: 1/20,000-1/100,000.
Western Blot: 1/2,000-1/10,000.
Immunochemistry: 1/1,000-1/5,000.
Reactivities Goat
Conjugation Unconjugated

Goat IgG (H&L) Antibody Biotin Conjugated

Applications WB: 1:500 - 1:5,000
IHC: 1:500 - 1:5,000
ELISA: 1:5,000 - 1:50,000
Reactivities Goat
Conjugation Biotin

Goat IgG (H&L) Antibody Peroxidase Conjugated

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Goat
Conjugation HRP

Human IgG (H&L) Antibody Alkaline Phosphatase Conjugated

Applications WB: 1:500-1:2,500
IHC: 1:200-1:1,000
ELISA: 1:2,000 - 1:10,000
Reactivities Human
Conjugation Alkaline Phosphatase

Goat IgG (H&L) Antibody

Applications WB: 1:2,000 - 1:10,000
IHC: 1:1,000 - 1:5,000
ELISA: 1:20,000 - 1:100,000
Reactivities Goat
Conjugation Unconjugated

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, PE

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation PE

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Biotin

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Biotin

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, FITC

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone I27/mAb27

Applications FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2).
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1 - 10 µg.
Reactivities Chicken
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone M15/8, FITC

Applications Flow cytometry.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone IA2, HRP

Applications ELISA: 1/500 - 1/1000; This HRP conjugated antibody may be used as a detection reagent in a sandwich ELISA for IgE, utilising SM1814P as capture reagent.
Reactivities Human
Conjugation HRP

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-8, Purified

Applications ELISA: 5 µg/ml (as a coating antibody).
Reactivities Rat
Conjugation Unconjugated

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, Biotin

Applications ELISA: 125ng/ml as detecting antibody.
Reactivities Rat
Conjugation Biotin

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, FITC

Applications Flow Cytometry: 10 µg/ml; use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC