CNY 6190.00
5周
Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), HRP
| Applications | To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Immunoperoxidase staining of cytoplasmic IgA2(m)2, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. General Recommended Dilutions: Histochemical Use: 1/10-1/50 ELISA: from 1/60 upwards. Western blot: from 1/20 upwards. |
| Reactivities | Human |
| Conjugation | HRP |



![Dot Blot showing the detection of Human IgG. A three-fold serial dilution of Human IgG starting at 200ng was spotted onto 0.45 ?m nitrocellulose. After blocking in 5% Blotto (p/n [B501-0500]) 1 Hour at 20°C, Anti-Human IgG (H&L) (MOUSE) Antibody Fluorescien Conjugated (p/n 609-302-002) secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged using the Bio-Rad VersaDoc® 4000 MP.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/609-302-002-human-igg-antibody-fluorescein-conjugated-1-db-4x3.jpg?browse)

