Secondary Antibodies

Human IgE mouse monoclonal antibody, clone NI 307, Purified

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgE Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100.
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgD mouse monoclonal antibody, clone NI 158, Purified

Applications To identify the presence of IgD in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgD Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/10-1/100
ELISA: from 1/25 upwards.
Western blot: from 1/50 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgE mouse monoclonal antibody, clone NI 307, Ascites

Applications To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgE and immunoblotting.
General Recommended Dilutions:
Histochemical 1/10-1/100.
ELISA: from 1/50 upwards.
Western blot: from 1/100 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), Azide Free

Applications To identify the presence of IgA2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA2, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200.
ELISA: from 1/200 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), Ascites

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, indirect Immunoperoxidase and Immunofluorescence staining of cytoplasmic IgA2(m)2, Haemagglutination and Immunoblotting.
General Recommended Dilutions:
Histochemical Use: 1/50-1/200
ELISA: from 1/200 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Unconjugated

Human IgE (Epsilon chain specific) mouse monoclonal antibody, clone HP6029, Purified

Applications ELISA: Purified (UNLB) antibody ≤1 ug/mL.
Western blotting
Dot- and slot-immunoblotting.
Immunocytochemistry.
Immunohistochemistry on Ffrozen Sections.
Reactivities Human
Conjugation Unconjugated

Human IgA (Secretory component) mouse monoclonal antibody, clone MC29/12, Purified

Applications Immunohistochemistry on frozen and paraffin sections.
ELISA.
Reactivities Human
Conjugation Unconjugated

Rabbit IgM (B Cell Marker) mouse monoclonal antibody, clone NRBM, Purified

Applications Immunohistochemistry on Frozen Sections.
Flow Cytometry:
Use 10 µl of diluted antibody to label 1x10e6 cells in 100 µl.
Reactivities Rabbit
Conjugation Unconjugated

Feline IgA mouse monoclonal antibody, clone IgA9-6A, Purified

Applications Western Blot.
ELISA: This antibody may be used as a capture antibody in an ELISA in combination with either SM1503B or AM01151BT-N as detecting antibody.
Reactivities Feline
Conjugation Unconjugated

Rat IgE mouse monoclonal antibody, clone MARE-1, Purified

Applications ELISA: 5 µg/ml; This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgE (SA013).
Western Blot.
Reactivities Rat
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 08C, Purified

Applications ELISA.
Immunoblotting.
Reactivities Chicken
Conjugation Unconjugated

Human IgG mouse monoclonal antibody, clone 4D2D9G8, Purified

Applications Western Blot: 1/500 - 1/2000.
ELISA: Propose dilution 1/10000.
Reactivities Human
Conjugation Unconjugated

Human IgM Fc5u Antibody

Applications WB: 1:1000-1:2000
ELISA: 1:1000 - 1:10000
Reactivities Human
Conjugation Unconjugated

Human IgG F(c) Antibody

Applications WB: 1:1000 - 1:2000
ELISA: 1:1000 - 1:10000
Reactivities Human
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone R1/69, Supernatant

Applications Immunohistochemistry on Formalin-Fixed Paraffin Embedded Sections: Use a dilution of 1/25-1/50 in an ABC method (30 minutes at room temperature).
Enzymatic predigestion with proteolytic enzymes is recommended for paraffin embedded tissue sections.
Recommended Positive Control: Tonsil.
Reactivities Human
Conjugation Unconjugated