Secondary Antibodies

Rat IgE mouse monoclonal antibody, clone MARE-1, FITC

Applications Flow Cytometry: Use 50 µl of 5 µg/ml diluted antibody to label 2 x 10e7 cells in 100 µl.
Reactivities Rat
Conjugation FITC

Chicken IgM (Mu heavy chain specific) mouse monoclonal antibody, clone M-1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells. (Ref.1)
Reactivities Chicken
Conjugation FITC

Human IgG (Fc heavy chain specific) mouse monoclonal antibody, clone EM-07, FITC

Applications Direct Immunofluorescence analysis: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone 4H10, FITC

Applications Flow cytometry: 2-5 µg/ml.
Reactivities Human
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 1C5, FITC

Applications FLISA: 1/200-1/400.
Flow Cytometry:
1 ≤ µg/106 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Chicken IgA (alpha chain specific) mouse monoclonal antibody, clone A-1, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence.
Reactivities Chicken
Conjugation FITC

Rat IgM (Mu chain specific) mouse monoclonal antibody, clone M 2A1, FITC

Applications Flow Cytometry: < / = 1.0 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgM+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2a (gamma 2a chain specific) mouse monoclonal antibody, clone 2A 8F4, FITC

Applications Flow Cytometry: < / = 1 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2a+ cells.
Reactivities Rat
Conjugation FITC

Rabbit (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Goat IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, FITC

Applications FLISA:1:200-1:400
Immunofluorescence < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgD (Delta chain specific) mouse monoclonal antibody, clone IADB6, FITC

Applications Indirect Immunofluorescent staining of IgD+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC

Human IgG (Fc specific) mouse monoclonal antibody, clone H2, FITC

Applications FLISA: 1/200-1/600.
Indirect Immunofluorescent staining
of IgG+ human B lymphocytes: ≤ 1 µg/106 cells.
Reactivities Human
Conjugation FITC

Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, FITC

Applications Indirect Immunofluorescent staining of IgM+ human B lymphocytes: < / = 1 µg/10e6 cells.
Reactivities Human
Conjugation FITC