Secondary Antibodies

Human IgA2 mouse monoclonal antibody, clone NI 512 (A89-038), FITC

Applications To identify the presence of IgA2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Immunofluorescence staining of cytoplasmic IgA2, and Immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation FITC

Human IgA2 (A2m2 Allotype Specific) mouse monoclonal antibody, clone NI 194-3 (A89-040), FITC

Applications To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as immunofluorescence staining of cytoplasmic IgA2(m)2, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
General Recommended Dilutions:
Histochemical Use: 1/20-1/100
Western blot: from 1/200 upwards.
Reactivities Human
Conjugation FITC

Human IgA (Fc specific) mouse monoclonal antibody, clone 15D6, FITC, Purified

Applications ELISA: 1/1000-1/10000.
Immunofluorescence.
Reactivities Human
Conjugation FITC

Rat IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone G1 7E7, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG1+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2b (Gamma-2b chain specific) mouse monoclonal antibody, clone 2B 10A8, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2b+ cells.
Reactivities Rat
Conjugation FITC

Rat IgG2c (gamma 2c chain specific) mouse monoclonal antibody, clone 2C 8F1, FITC

Applications Flow Cytometry: < / = 0.3 µg/10e6 cells.
Immunofluorescence Microscopy: Identification and enumeration of IgG2c+ cells.
Reactivities Rat
Conjugation FITC

Rabbit IgG (H&L) Antibody Fluorescein Conjugated Pre-Adsorbed

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Goat IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Reactivities Goat
Conjugation FITC

Human IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Reactivities Human
Conjugation FITC

Human IgG (H&L) Antibody Fluorescein Conjugated

Applications IF: 1:1,000 - 1:5,000
FC: 1:500 - 1:2,500
FLISA: 1:10,000 - 1:50,000
Conjugation FITC

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, FITC

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation FITC

Human IgM mouse monoclonal antibody, clone M15/8, FITC

Applications Flow cytometry.
Reactivities Human
Conjugation FITC

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, FITC

Applications Flow Cytometry: 10 µg/ml; use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgG2c (heavy chain) mouse monoclonal antibody, clone MARG2c-5, FITC

Applications Flow cytometry: 5 µg/ml; use 10 µl of diluted antibody to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgA (heavy chain) mouse monoclonal antibody, clone MARA-1, FITC

Applications Flow cytometry (5 µg/ml): use 50 µl of the suggested working dilution to label
10e6 cells in 100 µl.
Reactivities Rat
Conjugation FITC