Secondary Antibodies

Human IgA1 (alpha chain) mouse monoclonal antibody, clone B3506B4, AP

Applications Immunohistochemistry on frozen sections.
ELISA.
Western Blot.
Immunoblotting.
Reactivities Human
Conjugation AP

Human IgE mouse monoclonal antibody, clone 4H10, Purified

Applications Flow Cytometry: 4 µg/ml.
ELISA.
Western Blot.
Reactivities Human
Conjugation Unconjugated

Human IgG4 (pFC specific) mouse monoclonal antibody, clone NI 315 (HP 6206), Biotin

Applications To identify the presence of IgG4 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG4, and Immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used.
General Recommended Dilutions:
Histochemical Use: 1/50-1/250.
ELISA: from 1/100 upwards.
Western blot: from 1/500 upwards.
Reactivities Human
Conjugation Biotin

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, PE

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation PE

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, Biotin

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation Biotin

Human IgG (Fd region) mouse monoclonal antibody, clone HP6045, FITC

Applications ELISA (both capture and detection).
Western Blot.
Radioimmunoassay
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone I27/mAb27

Applications FACS surface, Western Blot, Basophil degranulation, Study of IgE binding to CD23 (Fc epsilon R2).
Usage recommendation
:
*This monoclonal antibody may be used between 1-10 μg/ml.
*Optimal dilution should be determined by each laboratory for each application.
*Coupled antibody: to maintain RT before use.
Reactivities Human
Conjugation Unconjugated

Chicken IgG / Chicken IgY mouse monoclonal antibody, clone 4G12, Purified

Applications Western Blot: 0.5 µg/ml for HRPO/ECL detection.
Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer.
ELISA: 0.1 µg/ml.
Immunoprecipitation: 1 - 10 µg.
Reactivities Chicken
Conjugation Unconjugated

Human IgM mouse monoclonal antibody, clone M15/8, FITC

Applications Flow cytometry.
Reactivities Human
Conjugation FITC

Human IgE mouse monoclonal antibody, clone IA2, HRP

Applications ELISA: 1/500 - 1/1000; This HRP conjugated antibody may be used as a detection reagent in a sandwich ELISA for IgE, utilising SM1814P as capture reagent.
Reactivities Human
Conjugation HRP

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-8, Purified

Applications ELISA: 5 µg/ml (as a coating antibody).
Reactivities Rat
Conjugation Unconjugated

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, Biotin

Applications ELISA: 125ng/ml as detecting antibody.
Reactivities Rat
Conjugation Biotin

Rat IgM (heavy chain) mouse monoclonal antibody, clone MARM-4, FITC

Applications Flow Cytometry: 10 µg/ml; use 50µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgG2b (heavy chain) mouse monoclonal antibody, clone MARG2b-3, Purified

Applications ELISA: 5ug/ml; This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgG2b (SA023).
Reactivities Rat
Conjugation Unconjugated

Rat IgG2a (heavy chain) mouse monoclonal antibody, clone MARG2a-1, Purified

Applications ELISA: 1.25 µg/ml; This antibody may be used as a coating antibody in ELISA in combination with clone MARK-1/MARL-15 as detection reagent and purified rat IgA (SA021).
Reactivities Rat
Conjugation Unconjugated