Secondary Antibodies
IgG (Immunglobulin G, light chain), mouse anti porcine, clone PHE425
| Applications | Tested for immunohistochemistry (IHC), ELISA and Western Blot, other applications not yet tested. Approximate working dilutions: IHC, frozen sections: 0.1µg/ml (1:2000) IHC, paraffin sections: 0.25µg/ml (1:800); Proteinase K pretreatment for antigen retrieval is recommended. Western Blot: 0.05 – 0.5µg/ml. ELISA: 20 - 50ng/ml (EC50 of 1ng/ml illustrated below) Optimal dilutions should be determined by the end user. |
| Reactivities | Porcine |
| Conjugation | Unconjugated |
CNY 3113.00
4周
Human IgM (Mu chain specific) mouse monoclonal antibody, clone SA-DA4, Purified
| Applications | Indirect Immunofluorescent staining of IgM+ human B lymphocytes: ≤ 1 µg/106 cells. ELISA. Immunohistochemistry on Frozen Sections. Western blotting. Dot- and Slot-Immunoblotting. Immunocytochemistry. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 3487.00
4周
Human IgG4 (pFC) mouse monoclonal antibody, clone HP6023 (also known as HO6023), HRP, Purified
| Applications | ELISA: 1/4000-1/8000 (See also Ref.2-9). Applications Reported in Literature: Flow Cytometry (Ref.15,18,19). Immunohistochemistry on Frozen Sections (Ref.10). Immunohistochemistry on Paraffin Sections (Ref.5,7,10,11). Immunocytochemistry (Ref.12). Immunoblotting (Ref.3,13-16). Microarray (Ref.17). Multiplex (Ref.2,20-22). Purification (Ref.15). |
| Reactivities | Human |
| Conjugation | HRP |
CNY 7710.00
5周
Human IgG3 (F(ab)2 specific) mouse monoclonal antibody, clone NI 330 (HP 6050), Purified
| Applications | To identify the presence of IgG3 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, Indirect Immunoperoxidase and Indirect Immunofluorescence staining of cytoplasmic IgG3, and Immunoblotting. General Recommended Dilutions: Histochemical Use: 1/50-1/200. ELISA: from 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
CNY 9630.00
5周
Human IgG2 (Fc specific) mouse monoclonal antibody, clone NI 25-1 (HP 6207), Biotin
| Applications | To identify the presence of IgG2 in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG2, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer’s choice have to be used. General Recommended Dilutions: Histochemical Use: 1/50-1/250. ELISA: from 1/100 upwards. Western blot: from 1/500 upwards. |
| Reactivities | Human |
| Conjugation | Biotin |
CNY 4400.00
4周
Human IgG1 (gamma 1 chain specific) mouse monoclonal antibody, clone 4E3, PE
| Applications | ELISA. FLISA (≤ 1.0 µg/ml). |
| Reactivities | Human |
| Conjugation | PE |
Human IgG4 mouse monoclonal antibody, clone MRQ-44, Purified
| Applications | Immunohistochemistry on Frozen and Formalin-Fixed, Paraffin-Embedded Sections: 1/100-1/500. Preparation and Pretreatment: 1. Cut 3-4 μm section of formalin-fixed paraffin-embedded tissue and place on positively charged slides; dry overnight at 58°C. 2. Deparaffinize, rehydrate, and epitope retrieve; the preferred method is the use of Heat Induced Epitope Retrieval (HIER) techniques in conjunction with a pressure cooker. The preferred method allows for simultaneous deparaffinization, rehydration, and epitope retrieval. Upon completion, rinse with 5 changes of distilled or deionized water. 3. If using HRP detection system, place slides in peroxide block for 10 minutes; rinse. If using AP detection system, omit this step. Positive Control: Tonsil. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgA (secretory) mouse monoclonal antibody, clone SC-05, Purified
| Applications | Western blot. Immunoprecipitation. Immunohistochemistry on Paraffin Sections. Immunohistochemistry on Frozen Sections. Examples of positive Human tissues: Secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland. |
| Reactivities | Human |
| Conjugation | Unconjugated |
Human IgE mouse monoclonal antibody, clone NI 307, FITC
| Applications | To identify the presence of IgE in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as direct and indirect immunofluorescence staining of cytoplasmic IgE and cell membrane IgE. General Recommended Dilutions: Histochemical Use: 1/50-1/200. |
| Reactivities | Human |
| Conjugation | FITC |
CNY 6190.00
5周
Human IgA (Secretory component) mouse monoclonal antibody, clone NI 194-4 (A89-039), Ascites
| Applications | To identify the presence of free or bound Secretory component in Human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as Radioimmunoassay, ELISA, Indirect Immunoperoxidase and Immunofluorescence staining, Haemagglutination and Immunoblotting. General Recommended Dilutions: Histochemical Use: from 1/50-1/200. ELISA: form 1/500 upwards. Western blot: from 1/1000 upwards. |
| Reactivities | Human |
| Conjugation | Unconjugated |
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