Secondary Antibodies

Equine IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10,000 - 1/100,000.
Reactivities Equine
Conjugation HRP

Canine IgM goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Reactivities Canine
Conjugation AP

Hamster IgG (adsorbed) goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/50; Use 50µl of the suggested working dilution to label 1 x 10e6 cells in 100µl.
Reactivities Hamster
Conjugation FITC

Human IgA + IgG + IgM goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of 0.1-0.5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Human
Conjugation FITC

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/25 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, PE

Applications Flow cytometry (1/5 - 1/10): use 50 µl of the suggested working dilution to label 10e6 cells in 100 µl.
Reactivities Rat
Conjugation PE

Mouse IgG (H+L chain), adsorbed goat polyclonal antibody, PE

Applications Flow Cytometry: Use 50 µl of 1/10 diluted antibody to label 10e6 cells in 100 µl.
Reactivities Mouse
Conjugation PE

Mouse IgG (adsorbed) goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/50-1/200 diluted antibody to label 1x106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC

F(ab')2 Rabbit IgG (H&L) Antibody Rhodamine Conjugated

Applications IF: 1:500-1:2,500
Conjugation TRITC

F(ab')2 Rat IgG (H&L) Antibody Texas Red™ Conjugated

Applications IF: 1:500-1:2,500
Conjugation Texas Red

Mouse IgG (H+L chain), F(ab)2 Fragment goat polyclonal antibody, Biotin

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Note: This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/10,000 to 1/150,000 of the reconstitution concentration is suggested for this product.
Recommended Dilutions
ELISA: 1/20,000-1/100,000.
Immunohistochemistry: 1/1,000-1/5,000.
Western blot: 1/2,000-1/10,000.
Reactivities Mouse
Conjugation Biotin

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays.
Recommended Dilutions:
ELISA: 1/10,000-1/150,000.
Western blot: 1/1,000-1/5,000.
Immunohistochemistry: 1/1,000-1/10,000.
Reactivities Chicken
Conjugation HRP

Hamster IgG goat polyclonal antibody, HRP

Applications Suitable for Immunoblotting (Western or dot blot), ELISA, Immunoperoxidase Electron Microscopy and Immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. This product has been assayed against 1.0 µg of Hamster IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:15,000 to 1:65,000 of the reconstitution concentration is suggested for this product.
Reactivities Hamster
Conjugation HRP