Secondary Antibodies

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Chicken IgA (Fc specific) Goat Polyclonal Antibody
AP21442AF-N

CNY 6580.00

5周

Chicken IgA (Fc specific) goat polyclonal antibody, Azide Free

Applications As unlabelled primary or secondary reagent for indirect detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. Can be used to prepare conjugates of choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000.
Immunohistochemistry: 1/50-1/250.
Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Antibody titre: Precipitin titre 1/64 when tested against pooled normal chicken serum in agar-block immunodiffusion titration.
Reactivities Chicken
Conjugation Unconjugated
Chicken IgA (Fc specific) Goat Polyclonal Antibody
AP21442BT-N

CNY 5640.00

5周

Chicken IgA (Fc specific) goat polyclonal antibody, Biotin

Applications Immunocytochemical and Immunohistochemical staining of IgA at the cellular and subcellular level of appropriately treated cell and tissue substrates.
To demonstrate circulating IgA antibodies in serodiagnostic microbiology and autoimmune diseases.
Identification of a specific antigen using a reference antibody of chicken origin known to be of the IgA isotype in the middle layer of the indirect test procedure.
Non-isotopic assay methodology (e.g. ELISA) to measure IgA in chicken serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user’s choice has to be used.
Antisera to IgA do not discriminate between serum IgA (monomeric and dimeric) and higher molecular forms such as secretory IgA.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Dilutions:
ELISA and comparable non-precipitating antibody-binding assays: 1/1,000-1/5,000.
Immunocytochemistry: 1/50-1/250.
Immunohistochemistry: 1/50-1/250.
Reactivities Chicken
Conjugation Biotin
Monkey IgA + IgG + IgM (H+L chain) Goat Polyclonal Antibody
AP31435AF-N

CNY 6580.00

5周

Monkey IgA + IgG + IgM (H+L chain) goat polyclonal antibody, Azide Free

Applications This IgG fraction allows the use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates; in radioimmunoassay; for the production of immunoconjugates with a selected marker; to prepare immunoaffinity adsorbents by coupling to an artificial carrier; in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent; in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.
Working dilutions:
For histochemical and cytochemical use are usually between 1/100 and 1/500.
In ELISA and comparable non-precipitating antibody-binding assays are between 1/1000 and 1/5000.
Reactivities Monkey
Conjugation Unconjugated
Mouse IgM (Fc specific) Goat Polyclonal Antibody
AP31543AF-N

CNY 6580.00

5周

Mouse IgM (Fc specific) goat polyclonal antibody, Azide Free

Applications Can be used as unlabelled primary or secondary reagent for indirect detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to prepare conjugates of the user’s own choice; to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry.
When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.
Typical working dilutions:
In histochemistry are usually between 1/50 and 1/250.
In ELISA and comparable non-precipitating antibody-binding assays between 1/500 and 1/5000.
Reactivities Mouse
Conjugation Unconjugated
Porcine IgM (Fc specific) Goat Polyclonal Antibody
AP33049BT-N

CNY 5640.00

5周

Porcine IgM (Fc specific) goat polyclonal antibody, Biotin

Applications Can be used:
• In immunocytochemical and immunohistochemical use for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates.
• To demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using an reference antibody of goat origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
• In non-isotopic assay methodology (e.g. ELISA) to measure IgM in swine serum or other body fluids.
• As a second step an avidin or streptavidin conjugate of the user’s choice has to be used. This immunoconjugate is not pre-diluted.
The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/100-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Porcine
Conjugation Biotin
Porcine IgM (Fc specific) Goat Polyclonal Antibody
AP33049HR-N

CNY 7710.00

5周

Porcine IgM (Fc specific) goat polyclonal antibody, HRP

Applications Can be used:
• In enzyme-immunocytochemical and immunohistochemical staining for the detection of IgM at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases.
• To identify a specific antigen using a reference antibody of swine origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
• In non-isotopic assay methodology (e.g. ELISA) to measure IgM in swine serum or other body fluids.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended Working Dilutions:
Histochemical and Cytochemical Use: 1/50-1/250.
ELISA and comparable non-precipitating antibody-binding assays: 1/1000-1/5000.
Reactivities Porcine
Conjugation HRP
Goat Monoclonal Anti-Rabbit IgG Fab antibody, clone RMG01
TA130059

CNY 6292.00

4周

Goat Monoclonal Anti-Rabbit IgG Fab antibody, clone RMG01

Applications ELISA: 0.01ug/mL – 0.5ug/mL; ICC/IHC: 0.5ug/mL-2ug/mL; WB (non-reduced): 0.1ug/mL-0.5ug/mL
Reactivities Rabbit IgG Fab
Conjugation Unconjugated
F(ab')2 Human IgA (alpha chain) Antibody Biotin Conjugated
TA398163

CNY 4988.00

4周

F(ab')2 Human IgA (alpha chain) Antibody Biotin Conjugated

Applications WB: 1:20,000 - 1:400,000
IHC: 1:500 - 1:5,000
IF: 1:200 - 1:1,000
FC: 1:200 - 1:1,000
ELISA: 1:20,000 - 1:400,000
Conjugation Biotin
Mouse lambda light chain Goat Polyclonal Antibody
SP2185P

CNY 7425.00

4周

Mouse lambda light chain goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/4000 - 1/8000.
Western Blot.
Immunofluorescence.
Immunoblotting.
Reactivities Mouse
Conjugation Unconjugated
Canine IgE Goat Polyclonal Antibody
SP2124P

CNY 7594.00

4周

Canine IgE goat polyclonal antibody, Purified

Applications ELISA: 1/100-1/10,000.
Western Blot: 1/1000-1/10,000.
Flow Cytometry: 1/200-1/2,000.
This antibody has been reported for use in Immunohistology of Frozen Canine Sections.
Reactivities Canine
Conjugation Unconjugated
Porcine IgG F(c) specific Goat Polyclonal Antibody
SP2017P

CNY 3200.00

4周

Porcine IgG F(c) specific goat polyclonal antibody, Aff - Purified

Applications ELISA: 1/100-1/1000.
Western Blot: 1/1000-1/30,000.
Immunodiffusion.
Reactivities Porcine
Conjugation Unconjugated
Feline IgM Goat Polyclonal Antibody
SP2014P

CNY 3200.00

4周

Feline IgM goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Feline
Conjugation Unconjugated
Porcine IgA Goat Polyclonal Antibody
SP2016APX

CNY 4620.00

4周

Porcine IgA goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Western Blot: 1/1000-1/10000.
Immunohistochemistry on Frozen Sections: 1/100-1/1000.
Reactivities Porcine
Conjugation AP
Rat IgG (H+L chain, adsorbed) Goat Polyclonal Antibody
SP1020P

CNY 3500.00

4周

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Purified

Applications ELISA.
Flow Cytometry: 5 µg/ml.
Immunoprecipitation: 1-5 µg/ml.
Reactivities Rat
Conjugation Unconjugated
Rat IgG (H+L chain, adsorbed) Goat Polyclonal Antibody
SP1020BS

CNY 4279.00

4周

Rat IgG (H+L chain, adsorbed) goat polyclonal antibody, Biotin

Applications

ELISA: 1/5000-1/20000.
Western Blot.
Immunofluorescence.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Rat
Conjugation Biotin