Chicken IgA (Fc specific) goat polyclonal antibody, Azide Free
| Applications | As unlabelled primary or secondary reagent for indirect detection of IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. Can be used to prepare conjugates of choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in nonisotopic methodology and solid phase immunochemistry. Recommended Dilutions: ELISA and comparable non-precipitating antibody-binding assays: 1/500-1/5,000. Immunohistochemistry: 1/50-1/250. Note: When applied in any Cytochemical or Histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used. Antibody titre: Precipitin titre 1/64 when tested against pooled normal chicken serum in agar-block immunodiffusion titration. |
| Reactivities | Chicken |
| Conjugation | Unconjugated |

![Western Blot of Goat Anti-Mouse IgG (H&L) Antibody. Lane M: Molecular Ladder. Lane 1: Mouse IgG whole molecule (p/n 010-0102). Lane 2: Mouse IgG F(c) Fragment (p/n 010-0103). Lane 3: Mouse IgG F(ab) Fragment (p/n 010-0105). Lane 4: Mouse IgM Kappa (p/n 010-0107). Lane 5: Mouse Serum (p/n [D308-05]). Load: 50ng per lane. Block: (p/n MB-070) for 30 min at RT. Primary Antibody: Anti-Mouse IgG (H&L) Antibody 1:1000 for 60 min at RT. Secondary antibody: HRP Donkey Anti-Goat IgG (p/n 705-703-125) 1:40,000 for 30 min at RT . Predicted/Observed Size: 28 and 55 kDa for Mouse IgG, F(c), F(ab), IgM Kappa, and Serum. Mouse F(c) migrates slightly higher.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/610-1102-anti-mouse-igg-1-wb-4x3.jpg?browse)
![SDS PAGE Results of Goat Anti-Mouse IgG Antibody. Lane 1: Goat Anti-Mouse IgG Reduced [1.0µg]. Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500).
Lane 3: Goat Anti-Mouse IgG Non-Reduced [1.0µg]. 4-20% Gel, Coomassie Stained.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/210-1102-gt-a-mouseigg-1-sds-4x3.jpg?browse)
![SDS PAGE Results of Goat Anti-Bovine IgG Antibody. Lane 1: Goat Anti-Bovine IgG Reduced [10µg].
Lane 2: Opal Prestained Molecular Weight Marker (p/n MB-210-0500). Lane 3: Goat Anti-Bovine IgG Non-Reduced [10µg].
4-20% Gel, Coomassie Stained.](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/201-1102-gt-a-bovine-igg-1-sds-4x3.jpg?browse)
![ELISA results of purified Goat Anti-Golden Syrian & Armenian Hamster IgG (Min X Mouse and Rat Serum Proteins) tested against purified Golden Syrian Hamster IgG and Armenian Hamster IgG. Each well was coated in duplicate with 1.0 µg of protein from different species. Golden Syrian Hamster IgG (Green Line), Armenian Hamster IgG (Red Line), Mouse IgG [p/n 010-0102] (Blue Line), and Rat IgG [012-0102] (Purple Line). The starting dilution of antibody was 5μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Blocking buffer (p/n MB-060-1000), Donkey Anti-Goat IgG HRP conjugated (p/n 605-703-125), and TMB substrate (p/n TMBE-1000).](https://cdn.origene.com/catalog/product/assets/images/antibody/secondary-antibody/115/620-101-440-gsah-igg-1-e-4x3.jpg?browse)

