Secondary Antibodies

Chicken IgM (Fc specific) goat polyclonal antibody, FITC

Applications Can be used in Immunocytochemical and Immunohistochemical staining of IgM at the cellular and subcellular level of appropriately treated cell and tissue substrates; to demonstrate circulating IgM antibodies in serodiagnostic microbiology and autoimmune diseases; to identify a specific antigen using a reference antibody of chicken origin known to be of the IgM isotype in the middle layer of the indirect test procedure.
This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.
Recommended working dilutions: 1/10 - 1/40, depending on the method used.
Reactivities Chicken
Conjugation FITC

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, FITC

Applications

FLISA.
Immunofluoresecence: ≤ 1 μg/106 cells.
Flow Cytometry.
Immunohistochemistry.

Reactivities Mouse
Conjugation FITC

Mouse IgG3 goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation FITC

Human IgD (heavy chain) goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Human
Conjugation FITC

Mouse IgG2a goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/10.
Immunofluorescence: 1/10.
Reactivities Mouse
Conjugation FITC

Mouse IgA goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of neat-1/10 diluted antibody to label 10e6 cells in 100 µl.
Immunofluorescence: neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse, Rat
Conjugation FITC

Mouse IgG2b goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of Neat-1/10 diluted antibody to label 106 cells in 100 µl.
Immunocytochemistry: Neat-1/10.
Immunohistochemistry on Frozen Sections.
Reactivities Mouse
Conjugation FITC

Rabbit IgG (H+L chain) (Fab Fragment) goat polyclonal antibody, FITC

Applications Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended dilutions:
Flow cytometry 1:2,000 - 1:10,000
Immunofluorescence 1:1,000 - 1:5,000
FLISA:1:10,000-1:50,000.
Reactivities Rabbit
Conjugation FITC

Canine IgE goat polyclonal antibody, FITC

Applications Flow Cytometry: 1:200 - 1:2,000.
Reactivities Canine
Conjugation FITC

Hamster IgG (adsorbed) goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/50; Use 50µl of the suggested working dilution to label 1 x 10e6 cells in 100µl.
Reactivities Hamster
Conjugation FITC

Human IgA + IgG + IgM goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 10 µl of 0.1-0.5 µg/ml diluted antibody to label 106 cells in 100 µl.
Reactivities Human
Conjugation FITC

Rat IgG (H+L chain), F(ab)2 Fragment, adsorbed goat polyclonal antibody, FITC

Applications Flow Cytometry: 1/25 - 1/100; Use 10µl of the suggested working dilution to label 10e6 cells in 100µl.
Reactivities Rat
Conjugation FITC

Mouse IgG (adsorbed) goat polyclonal antibody, FITC

Applications Flow Cytometry: Use 50 µl of 1/50-1/200 diluted antibody to label 1x106 cells in 100 µl.
Reactivities Mouse
Conjugation FITC