Secondary Antibodies

Mouse IgG1 goat polyclonal antibody, AP

Applications ELISA: 1/2000-1/4000.
Western Blot.
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation AP

Rabbit IgG (H+L chain) goat polyclonal antibody, AP, Liquid

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended Dilutions:
ELISA: 1/3,000-1/25,000.
Western blot: 1/1,000-1/4,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Rabbit
Conjugation AP

Mouse IgG (Fc specific) goat polyclonal antibody, AP

Applications Suitable for Immunoprecipitation, Immunodiffusion, conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
ELISA: 1/2,000-1/100,000
Western blot: 1/500-1/2,500
Immunohistochemistry: 1/200-1/1,000.
Note: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate code #NPP-10 as a substrate for 30 minutes at room temperature. A working dilution of 1/500 to 1/5,000 of the reconstitution concentration is suggested for this product.
Reactivities Mouse
Conjugation AP

Human IgE goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000.
Western Blot: 1/1000-1/5000.
Reactivities Human, Monkey
Conjugation AP

Mouse IgA+IgG+IgM (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000. 
Immunoblotting. 
Immunohistochemistry.
Reactivities Mouse
Conjugation AP

Mouse IgG goat polyclonal antibody, AP, Aff - Purified

Applications ELISA: 1/10000.
Western Blot: 1/5000-1/10000,
Immunohistochemistry.
Reactivities Mouse
Conjugation AP

Mouse IgG1 (Ads. to Hu, Bov, Rb Serum Proteins) goat polyclonal antibody, AP, Aff - Purified

Applications Anti-Mouse IgG1 Alkaline Phosphatase Conjugated Secondary antibodies are ideal for western blotting, Immunohistochemistry and ELISA as well as other antibody detection methods.
Recommended Dilutions:
ELISA: 1/10,000-1/40,000.
Western Blot: 1/200-1/2,000. 
Immunohistochemistry: 1/200-1/1,000.
Reactivities Mouse
Conjugation AP

Mouse IgA (alpha chain specific) goat polyclonal antibody, AP

Applications

ELISA: 1/2,000-1/4,000. 
Immunoblotting. 
Immunohistochemistry.

Reactivities Mouse
Conjugation AP

Mouse IgG2b goat polyclonal antibody, AP

Applications ELISA: 1/2000-1/4000.  
Western Blot. 
Immunohistochemistry on Frozen Sections.
Immunohistochemistry on Paraffin Sections.
Reactivities Mouse
Conjugation AP

Porcine IgA goat polyclonal antibody, AP

Applications ELISA: 1/1000-1/10000. 
Western Blot: 1/1000-1/10000. 
Immunohistochemistry on Frozen Sections: 1/100-1/1000.
Reactivities Porcine
Conjugation AP

Human IgM (Mu chain specific) goat polyclonal antibody, AP

Applications Suitable for Immunoblotting (Western or Dot blot), ELISA and Immunohistochemistry as well as other AP-antibody based enzymatic assays requiring lot-to-lot consistency.
Recommended dilutions
ELISA: 1/1,000-1/3,000.
Western blot: 1/500-1/2,000.
Immunohistochemistry: 1/200-1/1,000.
Reactivities Human
Conjugation AP

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, AP

Applications ELISA: In Sandwich ELISA a 1/800 dilution of GGHL-30PH-2 was found to generate an O.D of 1.0 in a 20 minute reaction with p-Nitrophenyl Phosphate as the substrate.
Western Blot (1/1000).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After separation, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Quality Control: Antibodies were analyzed using Immunoelectrophoresis and a Sandwich ELISA.
Reactivities Chicken
Conjugation AP

Rabbit IgA goat polyclonal antibody, AP

Applications ELISA: 1/100-1/1000.
Reactivities Rabbit
Conjugation AP

Mouse IgG (adsorbed) goat polyclonal antibody, AP

Applications ELISA: 1/200-1/2500.
Western Blot: 1/100-1/500.
Reactivities Mouse
Conjugation AP

Porcine IgG F(c) specific goat polyclonal antibody, AP

Applications ELISA: 1/5000-1/50000.
Reactivities Porcine
Conjugation AP