Secondary Antibodies

Mouse IgM (Mu chain specific), adsorbed goat polyclonal antibody, Purified

Applications ELISA: 5µg/ml as coating antibody.
Reactivities Mouse
Conjugation Unconjugated

Human IgG (H+L chain), Fab Fragment goat polyclonal antibody, FITC

Applications Suitable for Immunomicroscopy and Flow Cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Recommended Dilutions:
FLISA: 1/10,000-1/50,000.
Flow Cytometry: 1/500-1/2,500.
IF Microscopy: 1/1,000-1/5,000.
Reactivities Human
Conjugation FITC

Turkey IgG (H+L chain) goat polyclonal antibody, AP

Applications ELISA: 1/2,000-1/4,000.
Reactivities Turkey
Conjugation AP

Turkey IgG (H+L chain) goat polyclonal antibody, HRP

Applications ELISA: 1/4,000-1/8,000.
Reactivities Turkey
Conjugation HRP

Mouse IgG goat polyclonal antibody, FITC, Aff - Purified

Applications Immunofluorescence: 1/500-1/1000. 
Immunohistochemistry.
Reactivities Mouse
Conjugation FITC

Mouse IgG3 goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Mouse
Conjugation Unconjugated

Chicken IgG / Chicken IgY (H+L chain) goat polyclonal antibody, FITC

Applications Immunocytochemistry (1/200).
In this latter test, abundant Fluorescence staining was observed in 3T3 cells after blocking with BlokHen® (1/10 dilution in PBS), incubation with Chicken anti-Vimentin antibodies (1/1000 dilution in PBS) and then incubation with GGHL-30F-2 (1/200 dilution in PBS).
Immunoelectrophoresis: Chicken serum (3 µl) was placed in the center well (at the clear circle) and then subjected to electrophoresis. After electrophoresis was complete, Goat anti-Chicken serum (75 μl) was placed in the lower trough and GGHL-30 (75 μl) (pre-conjugated affinity-purified antibody) was placed in the upper trough. After overnight incubation at 4°C, the gel was washed, fixed and stained with Coomassie. Note the single precipitin line between the center well and upper trough.
Reactivities Chicken
Conjugation FITC

PrecipHen® (Chicken IgY) goat polyclonal antibody, Agarose

Applications Immunoprecipitation.
Reactivities Chicken
Conjugation Agarose

Rabbit IgA goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Rabbit
Conjugation HRP

Rabbit IgA goat polyclonal antibody, AP

Applications ELISA: 1/100-1/1000.
Reactivities Rabbit
Conjugation AP

Human IgD (heavy chain) goat polyclonal antibody, Purified

Applications ELISA.
Reactivities Human
Conjugation Unconjugated

Mouse IgG2a goat polyclonal antibody, PE

Applications Flow Cytometry: 1/20 - 1/30.
Reactivities Mouse
Conjugation PE

Mouse IgG1 goat polyclonal antibody, PE

Applications Flow Cytometry: 1/20 - 1/30.
Reactivities Mouse
Conjugation PE

Mouse IgG2c goat polyclonal antibody, FITC

Applications Flow Cytometry.
Reactivities Mouse
Conjugation FITC

Mouse IgG2c goat polyclonal antibody, HRP

Applications ELISA: 1/10000-1/100000.
Reactivities Mouse
Conjugation HRP