Mouse IgG (H&L) Antibody Peroxidase Conjugated
| Applications | WB: 1:2,000 to 1:10,000 IHC: 1:500 - 1:2,500 ELISA: 1:70,000 |
| Reactivities | Mouse |
| Conjugation | HRP |
Secondary Antibodies
Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, HRP
| Applications | Suitable for Immunoblotting (1/2,000-1/20,000 for Western blot), ELISA (1/180,000), Immunoperoxidase electron microscopy and Immunohistochemistry (1/1,000-1/5,000) as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/20,000 to 1/80,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | HRP |
Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, Aff - Purified
| Applications | Suitable for Immunoprecipitation, Immunodiffusion, Conjugation and most immunological methods requiring lot-to-lot consistency, high titer and specificity. ELISA : 1:20,000 - 1:100,000 Western Blot : 1:2,000 - 1:10,000 Immunohistochemistry: 1:1,000 - 1:5,000 |
| Reactivities | Mouse |
| Conjugation | Unconjugated |
Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, Texas Red
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Reactivities | Mouse |
| Conjugation | Texas Red |
Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, TRITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Reactivities | Mouse |
| Conjugation | TRITC |
Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, FITC
| Applications | Suitable for Immunomicroscopy and Flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. |
| Reactivities | Mouse |
| Conjugation | FITC |
Mouse IgG (H+L chain), adsorbed donkey polyclonal antibody, AP
| Applications | Suitable for Immunoblotting (western or dot blot), ELISA and immunohistochemistry as well as other phosphatase-antibody based enzymatic assays requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA usingp-nitrophenyl phosphate (pNPP) as a substrate for 30 minutes at room temperature. A working dilution of 1:500 to 1:2,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | AP |
Mouse IgG (H+L chain), Fab Fragment donkey polyclonal antibody, Biotin
| Applications | Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency. Recommended Dilutions: This product has been assayed against 1.0 µg of Mouse IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:50,000 to 1:200,000 of the reconstitution concentration is suggested for this product. |
| Reactivities | Mouse |
| Conjugation | Biotin |
Mouse IgG (H+L chain), F(ab)2 Fragment, adsorbed donkey polyclonal antibody, PE
| Applications | Suitable for Immunomicroscopy and Flow Cytometry or FACS as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. Flow Cytometry: the maximum amount of reagent required to stain 1x10e6 cells is approx. 1.0 µg of antibody conjugate. As a general guideline dilutions of 1/100 to 1/250 should be suitable for most applications. |
| Reactivities | Mouse |
| Conjugation | PE |
