AGL Human shRNA Plasmid Kit (Locus ID 178)
CAT#: TL314895
AGL - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector, 5µg of each construct provided
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CNY 7,740.00
货期*
2周
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Specifications
Product Data | |
Product Name | AGL Human shRNA Plasmid Kit (Locus ID 178) |
Locus ID | 178 |
UniProt ID | P35573 |
Synonyms | GDE |
Vector | pGFP-C-shLenti |
Format | Lentiviral plasmids |
Kit Components | AGL - Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 178). 5µg purified plasmid DNA per construct29-mer scrambled shRNA cassette in pGFP-C-shLenti Vector, TR30021, included for free. |
RefSeq | NM_000028, NM_000642, NM_000643, NM_000644, NM_000645, NM_000646, NM_000642.1, NM_000642.2, NM_000645.1, NM_000645.2, NM_000643.1, NM_000643.2, NM_000644.1, NM_000644.2, NM_000028.1, NM_000028.2, NM_000646.1, NM_000646.2, BC078663, BC078663.1, BC020705, BC057220, NM_000642.3 |
Summary | This gene encodes the glycogen debrancher enzyme which is involved in glycogen degradation. This enzyme has two independent catalytic activities which occur at different sites on the protein: a 4-alpha-glucotransferase activity and a amylo-1,6-glucosidase activity. Mutations in this gene are associated with glycogen storage disease although a wide range of enzymatic and clinical variability occurs which may be due to tissue-specific alternative splicing. Alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008] |
shRNA Design | These shRNA constructs were designed against multiple splice variants at this gene locus. To be certain that your variant of interest is targeted, please contact techsupport@origene.com. If you need a special design or shRNA sequence, please utilize our custom shRNA service. |
Performance Guaranteed | OriGene guarantees that the sequences in the shRNA expression cassettes are verified to correspond to the target gene with 100% identity. One of the four constructs at minimum are guaranteed to produce 70% or more gene expression knock-down provided a minimum transfection efficiency of 80% is achieved. Western Blot data is recommended over qPCR to evaluate the silencing effect of the shRNA constructs 72 hrs post transfection. To properly assess knockdown, the gene expression level from the included scramble control vector must be used in comparison with the target-specific shRNA transfected samples. For non-conforming shRNA, requests for replacement product must be made within ninety (90) days from the date of delivery of the shRNA kit. To arrange for a free replacement with newly designed constructs, please contact Technical Services at techsupport@origene.com. Please provide your data indicating the transfection efficiency and measurement of gene expression knockdown compared to the scrambled shRNA control (Western Blot data preferred). |
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