NS1 (NC_012729) Virus Tagged ORF Clone

CAT#: VC100552

  • TrueORF®

Myc-DDK-tagged ORF clone of viral ORF for non structural protein NS1 [Human bocavirus 4], codon optimized for human cell expression, YP_002916060



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CNY 7,700.00


货期*
4周

规格
    • 10 ug

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经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 100 ul

CNY 600.00


Clone OTI4C5, Anti-DDK (FLAG) monoclonal antibody
    • 100 ul

CNY 1,050.00

Specifications

Product Data
Type Virus Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>The Viral ORF clone VC100552 represents NCBI reference of YP_002916060 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s)

GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTTCTCTGCGCCCGTTCTGAGGGCCTTCTCTCAGCCCACCTTTACCTACGTCATTAAATTCCCTT
ACAATAACTGGAAAGAGGACGAGCACCTTCTGTGGTCCCTGCTTGCTCCGGGCACAGAATCCCTGATGAT
CCAGCTCAAAAACTGCGCCCCTCATCCTGAAGATGACCCAATCAGAGAAGATATCCTCTGCAGCCTGGCT
GACCTCCATTACGGGGCTGTTTTCGCTAAAGCCTGTTATATAGCCACTTCCACATTGATGGGCCAAAAGC
AGAGGACTCTGTTCCCTCGCTGTGATATTGTGTGTCAATCTGAAATTGGAAGTGATTTTCTCCATTGTCA
CATACTCGTGGGAGGAGCCGGCCTCAGCAAACGCAATGCCAAAATTTCCAGAGCAACTCTTTTGGGTTTG
GTAATGGCAGAACTTACCCAGAGATGCAAACTCCTTCTTGCTCACAGGCCCTTTGAGCCGGCGGAGGCCA
CCATCTACCATGAACTGAAAAGAATCGAAAGAGAAGCTTGGTCTGGACACACCGGGAACTGGGTGCAGAT
TCTGCAATATAAAGACAAGAGAGGCGACCTCCATGCCCAACCTATTGACCCACTGCGGTTCCTGAAACAT
TATATCCTGCCGAAGAACCGACTCATCAGCCCAAGCAGCAAGCCTGATGTCTGCACTTCCCCCGATAATT
GGTTCATCCTCGCGGATAAGACATACTCCCACACAATTATTAATGGTCTCCCCCTGCTGGAAAGGAACCG
GAAGGCCTACCTGCAGGAGTTGGAAAGCGAGGTCATTCCCGGACCGTCCGCTATGGCATTCGGCGGCAGA
GGGGCCTGGGAGCAGCTCCCCGAGGTGGGGGAACAGAGGCTGATCACAAGTAACACCTCCACCGCTTACA
AGGCGAATAAAAAGGAAAAGTTGATGCTGAACCTGCTGGACAAGTGCGATGAGCTTAATCTCCTCGTGTA
TGAGGACCTGGTGTCCGCGTGCCCAGACCTGCTCTTGATGCTCGAGGGCCAACCTGGTGGAGCAAGGCTG
ATTGAGCAGGTCCTGGGAATGCACCATATTAAAGTGTGCGCCAAACACACAGCCCTGAGTTTCTTGTTCC
ACCTTCACCCAGACCAACTGCTGACGTCAAGCAATAAAGCACTCAAATTGCTGCTCATTCAGGGATATAA
CCCTCTGCAAGTCGGACATGCTATCTGCTGCGTCCTCAATAAGCAAATGGGAAAGCAGAACACCATCTGT
TTTTATGGCCCTGCATCTACCGGCAAAACCAACTTCGCCAAGGCCATAGTCCAGGGGGTCAGACTCTATG
GGTGTGTAAACCACTTGAATAAGGGGTTCGTGTTTAACGATTGCCGCCAAAGACTGATCATCTGGTGGGA
AGAATGCCTTATGCACCAGGACTGGGTCGAGCCCGCGAAATGTATTCTGGGCGGCACCGAGTGCCGAATT
GATGTCAAGCACAAGGACAGTGTCCTTTTGCAGCAGACCCCTGTTATCATCAGCACCAACCACGATATAT
ACTCCGTCGTAGGAGGAAACACAGTGTCTCACGTCCACGCGGCCCCTCTGAAGGAACGAGTGCTCCAGCT
TAACTTTATGAAGCAGCTGCCTCAGACCTTCGGCGAAATCTCACCAAGCGAGATCGCAGAACTTCTGCAA
TGGTGTTTTAACGAGTACGACTGTACCCTGGCTGGCTTTAAACAGAAATGGAACTTGGACAAGGTGCCAA
ATAGCTTTCCGATCGGAGATTTGTGCCCAACCCACAGTCAGGACTTTACACTTCACGAAAATGGCTTCTG
TAGCGACTGCGGCGGTTACCTGCCCCATAGCGCCGATGATAGCGTGTACACAGACGTGGCCAGCGAAACT
ACGTCAGGAGATTACGATCCAGGCCGGCTT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>VC100552 representing YP_002916060
Red=Cloning sites Green=Tags

MAFSAPVLRAFSQPTFTYVIKFPYNNWKEDEHLLWSLLAPGTESLMIQLKNCAPHPEDDPIREDILCSLA
DLHYGAVFAKACYIATSTLMGQKQRTLFPRCDIVCQSEIGSDFLHCHILVGGAGLSKRNAKISRATLLGL
VMAELTQRCKLLLAHRPFEPAEATIYHELKRIEREAWSGHTGNWVQILQYKDKRGDLHAQPIDPLRFLKH
YILPKNRLISPSSKPDVCTSPDNWFILADKTYSHTIINGLPLLERNRKAYLQELESEVIPGPSAMAFGGR
GAWEQLPEVGEQRLITSNTSTAYKANKKEKLMLNLLDKCDELNLLVYEDLVSACPDLLLMLEGQPGGARL
IEQVLGMHHIKVCAKHTALSFLFHLHPDQLLTSSNKALKLLLIQGYNPLQVGHAICCVLNKQMGKQNTIC
FYGPASTGKTNFAKAIVQGVRLYGCVNHLNKGFVFNDCRQRLIIWWEECLMHQDWVEPAKCILGGTECRI
DVKHKDSVLLQQTPVIISTNHDIYSVVGGNTVSHVHAAPLKERVLQLNFMKQLPQTFGEISPSEIAELLQ
WCFNEYDCTLAGFKQKWNLDKVPNSFPIGDLCPTHSQDFTLHENGFCSDCGGYLPHSADDSVYTDVASET
TSGDYDPGRL

TRTRRLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites SgfI-MluI      Cloning Scheme for this gene     
ACCN NC_012729
ORF Size 1920 bp
OTI Disclaimer The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NC_012729.2, YP_002916060
RefSeq ORF 1920 bp
Locus ID 7922600
MW 71.8 kDa
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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