L4 (NC_001405) Virus Tagged ORF Clone
CAT#: VC100386
- TrueORF®
Myc-DDK-tagged ORF clone of viral ORF for hexon assembly protein 100K [Human adenovirus C], codon optimized for human cell expression, NP_040528
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CNY 12,260.00
Product images
CNY 600.00
CNY 1,050.00
Specifications
Product Data | |
Type | Virus Tagged ORF Clone |
Tag | Myc-DDK |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>The Viral ORF clone VC100386 represents NCBI reference of NP_040528 with codon optimized for human cell expression
Red=Cloning site Blue=ORF Green=Tags(s) GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAGCGTGGAAAAAGAGGATAGCCTTACGGCCCCCTTCGAGTTCGCAACAACCGCATCCACCGACG CAGCAAACGCGCCCACTACCTTTCCTGTGGAAGCTCCCCCCTTGGAGGAGGAGGAGGTTATCATCGAGCA GGACCCTGGATTTGTGAGCGAAGACGATGAGGATCGCTCAGTTCCAACCGAGGACAAAAAGCAAGATCAG GACGACGCTGAGGCCAACGAGGAGCAGGTGGGGAGAGGGGACCAACGCCACGGGGACTATCTTGATGTCG GGGACGATGTGCTGCTCAAACACCTGCAACGCCAGTGTGCCATCATTTGTGATGCCCTTCAGGAGAGGAG TGACGTTCCCCTCGCTATCGCCGATGTGAGCCTGGCATATGAACGCCATTTGTTCAGCCCTCGAGTCCCC CCTAAGAGGCAGGAGAATGGAACTTGTGAACCTAACCCCAGACTTAACTTCTACCCAGTGTTTGCCGTGC CCGAAGTCCTCGCTACCTACCACATCTTTTTCCAGAATTGTAAGATCCCCCTGTCATGTCGCGCGAACCG GAGCCGGGCAGATAAACAGCTTGCACTTCGCCAGGGCGCGGTGATTCCCGATATTGCCAGCCTGGATGAG GTACCAAAGATTTTTGAGGGACTGGGACGCGACGAAAAGCGAGCCGCCAATGCACTGCAGCAGGAGAATA GCGAAAATGAAAGTCACTGCGGCGTCCTCGTTGAGCTGGAGGGTGATAACGCCCGCCTGGCGGTGCTCAA GAGAAGCATAGAAGTAACCCATTTCGCCTACCCAGCCCTCAATCTGCCACCTAAAGTTATGAGTACCGTC ATGTCAGAGTTGATTGTGAGAAGGGCAAGACCTCTGGAGAGGGACGCTAACCTCCAGGAACAAACCGAAG AGGGACTCCCAGCCGTGGGCGACGAACAGCTTGCCCGCTGGCTCGAAACTCGGGAGCCGGCTGATCTCGA AGAGCGACGGAAACTGATGATGGCCGCGGTGCTGGTGACTGTCGAACTCGAGTGCATGCAGAGGTTCTTT GCAGACCCGGAAATGCAGAGAAAGCTGGAGGAGACACTGCACTATACATTCCGCCAGGGATACGTACGGC AGGCATGTAAAATCTCCAACGTTGAGCTCTGCAACCTGGTATCCTACCTGGGGATTCTCCACGAGAATAG GCTGGGACAGAATGTGCTTCACAGTACGTTGAAAGGAGAGGCAAGACGGGATTATGTGAGAGACTGTGTG TATTTGTTCCTGTGCTACACTTGGCAGACTGCGATGGGTGTGTGGCAGCAGTGTCTCGAGGAGCGCAACC TGAAAGAACTGCAGAAGCTGCTCAAACAGAACCTGAAAGATCTGTGGACTGCATTCAATGAGCGCAGTGT TGCAGCACACCTTGCCGATATTATCTTTCCGGAAAGGCTGCTCAAGACCCTGCAGCAGGGCCTGCCCGAC TTCACCTCTCAGTCAATGCTGCAGAACTTCCGAAATTTTATACTGGAGCGGAGCGGCATCCTGCCTGCGA CCTGCTGTGCTCTCCCCAGCGACTTCGTGCCGATCAAATATCGCGAGTGTCCTCCCCCTCTGTGGGGACA CTGCTATCTGCTGCAGCTGGCTAACTATCTTGCCTATCACTCAGACATCATGGAGGACGTCAGCGGAGAC GGACTGCTGGAGTGTCATTGCAGGTGCAACCTCTGTACACCCCACAGGAGTCTCGTATGCAACAGCCAGC TGTTGAGCGAGTCCCAGATCATTGGTACCTTTGAGCTGCAAGGACCTTCCCCGGACGAAAAAAGCGCCGC CCCCGGGCTCAAACTGACCCCCGGCCTGTGGACTTCCGCATATCTGCGGAAGTTTGTCCCAGAGGACTAT CACGCTCATGAAATCCGCTTCTATGAAGACCAGTCCAGACCTCCTAACGCGGAGCTGACCGCATGCGTGA TTACCCAGGGACATATCCTGGGCCAGCTGCAGGCTATCAATAAGGCGCGGCAGGAGTTTCTCCTGAGGAA GGGCCGCGGAGTGTATCTTGACCCTCAGTCCGGAGAAGAGTTGAACCCTATTCCACCCCCCCCCCAGCCA TACCAACAGCCAAGAGCACTCGCCTCCCAGGACGGAACCCAGAAGGAGGCAGCAGCCGCAGCAGCAGCAA CTCATGGTAGAGGGGGTATTCTCGGCCAGTCTGGCAGGGGCGGATTCGGCCGCGGAGGTGGGGACGATGG AAGATTGGGCCAGCCTAGAAGGAGCTTTAGAGGTAGGCGAGGCGTCAGGAGAAACACCGTGACCCTCGGC AGGATACCACTTGCGGGAGCCCCCGAGATTGGGAACCGGAGCCAGCACAGGTACAATCTGCGGTCATCTG GCGCCGCAGGCACTGCCTGTTCCCCAACTCAGCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >VC100386 representing NP_040528
Red=Cloning sites Green=Tags MESVEKEDSLTAPFEFATTASTDAANAPTTFPVEAPPLEEEEVIIEQDPGFVSEDDEDRSVPTEDKKQDQ DDAEANEEQVGRGDQRHGDYLDVGDDVLLKHLQRQCAIICDALQERSDVPLAIADVSLAYERHLFSPRVP PKRQENGTCEPNPRLNFYPVFAVPEVLATYHIFFQNCKIPLSCRANRSRADKQLALRQGAVIPDIASLDE VPKIFEGLGRDEKRAANALQQENSENESHCGVLVELEGDNARLAVLKRSIEVTHFAYPALNLPPKVMSTV MSELIVRRARPLERDANLQEQTEEGLPAVGDEQLARWLETREPADLEERRKLMMAAVLVTVELECMQRFF ADPEMQRKLEETLHYTFRQGYVRQACKISNVELCNLVSYLGILHENRLGQNVLHSTLKGEARRDYVRDCV YLFLCYTWQTAMGVWQQCLEERNLKELQKLLKQNLKDLWTAFNERSVAAHLADIIFPERLLKTLQQGLPD FTSQSMLQNFRNFILERSGILPATCCALPSDFVPIKYRECPPPLWGHCYLLQLANYLAYHSDIMEDVSGD GLLECHCRCNLCTPHRSLVCNSQLLSESQIIGTFELQGPSPDEKSAAPGLKLTPGLWTSAYLRKFVPEDY HAHEIRFYEDQSRPPNAELTACVITQGHILGQLQAINKARQEFLLRKGRGVYLDPQSGEELNPIPPPPQP YQQPRALASQDGTQKEAAAAAAATHGRGGILGQSGRGGFGRGGGDDGRLGQPRRSFRGRRGVRRNTVTLG RIPLAGAPEIGNRSQHRYNLRSSGAAGTACSPTQP TRTRRLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NC_001405 |
ORF Size | 2415 bp |
OTI Disclaimer | The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decoded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NC_001405.1, NP_040528 |
RefSeq ORF | 2415 bp |
Locus ID | 2653005 |
MW | 90.1 kDa |
Documents
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FAQs |
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Resources
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