P2X2 (P2RX2) (NM_001282164) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DFNA41; P2X2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC335678 representing NM_001282164. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCCGCCGCCCAGCCCAAGTACCCCGCCGGGGCGACCGCCCGGCGCCTGGCCCGGGGCTGCTGGTCC GCCCTCTGGGACTACGAGACGCCCAAGGTGATCGTGGTGAGGAACCGGCGCCTGGGGGTCCTGTACCGC GCCGTGCAGCTGCTCATCCTGCTCTACTTCGTGTGGTACGTATTCATCGTGCAGAAAAGCTACCAGGAG AGCGAGACGGGCCCCGAGAGCTCCATCATCACCAAGGTCAAGGGGATCACCACGTCCGAGCACAAAGTG TGGGACGTGGAGGAGTACGTGAAGCCCCCCGAGAGCATAAGGGTCCACAACGCCACCTGCCTCTCCGAC GCCGACTGCGTGGCTGGGGAGCTGGACATGCTGGGAAACGGGGCCCTCCAAGACCTGCGAGGTGTTCGG CTGGTGCCCGGTGGAAGATGGGGCCTCTGTCAGCCAATTTCTGGGTACGATGGCCCCAAATTTCACCAT CCTCATCAAGAACAGCATCCACTACCCCAAATTCCACTTCTCCAAGGGCAACATCGCCGACCGCACAGA CGGGTACCTGAAGCGCTGCACGTTCCACGAGGCCTCCGACCTCTACTGCCCCATCTTCAAGCTGGGCTT TATCGTGGAGAAGGCTGGGGAGAGCTTCACAGAGCTCGCACACAAGGCAGGGGTGGTGTCATCGGGGTC ATTATCAACTGGGACTGTGACCTGGACCTGCCTGCATCGGAGTGCAACCCCAAGTACTCCTTCCGGAGG CTTGACCCCAAGCACGTGCCTGCCTCGTCAGGCTACAACTTCAGGTTTGCCAAATACTACAAGATCAAT GGCACCACCACCCGCACGCTCATCAAGGCCTACGGGATCCGCATTGACGTCATTGTGCATGGACAGGCC GGGAAGTTCAGCCTGATTCCCACCATTATTAATCTGGCCACAGCTCTGACTTCCGTCGGGGTGGGCTCC TTCCTGTGCGACTGGATCTTGCTAACATTCATGAACAAAAACAAGGTCTACAGCCATAAGAAATTTGAC AAGATGGTGGACACTCCTGCCTCCGAGCCTGCCCAAGCCTCCACACCCACAGACCCCAAAGGTTTGGCT CAACTCTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001282164
Insert Size	1113 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001282164.1
RefSeq Size	1564 bp
RefSeq ORF	1113 bp
Locus ID	22953
UniProt ID	Q9UBL9
Protein Families	Druggable Genome, Ion Channels: ATP Receptors, Transmembrane
Protein Pathways	Calcium signaling pathway, Neuroactive ligand-receptor interaction
MW	41.4 kDa
Gene Summary	The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Multiple transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Aug 2013] Transcript Variant: This variant (7) lacks three alternate in-frame segments in the 5' and 3' ends and two alternate segments in the middle compared to variant 4, that cause a frameshift. The resulting isoform (J) lacks three alternate segments and contains a different internal segment compared to isoform D.

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.