DYNC1I2 (NM_001271789) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	DIC74; DNCI2; IC2; NEDMIBA
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC333115 representing NM_001271789. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGTCAGACAAAAGTGAATTAAAGGCTGAGTTGGAACGTAAGAAGCAGCGACTGGCCCAAATCAGAGAG GAAAAGAAGAGAAAAGAAGAAGAAAGGAAAAAAAAAGAAACAGACCAGAAGAAGGAAGCTGTTGCTCCT GTGCAAGAAGAATCAGATCTTGAAAAAAAAAGGAGAGAAGCTGAAGCATTGCTTCAAAGCATGGGGCTA ACTCCAGAATCCCCCATTGTCCCTCCTCCTATGTCTCCATCCTCCAAATCTGTGAGCACTCCAAGTGAA GCTGGAAGCCAAGACTCTGGAGATGGCGCCGTGGGATCTAGACGAGGACCTATTAAACTTGGAATGGCT AAAATCACGCAAGTCGACTTTCCTCCTCGAGAAATTGTCACGTATACAAAGGAAACTCAGACTCCAGTT ATGGCTCAACCCAAAGAAGATGAAGAGGAAGATGATGATGTAGTGGCTCCTAAACCACCTATTGAACCT GAAGAAGAGAAAACTTTAAAGAAAGATGAGGAAAATGATAGTAAAGCTCCCCCTCATGAGCTGACTGAA GAAGAAAAGCAACAAATCTTGCACTCTGAGGAATTTTTAAGTTTCTTTGACCATTCTACAAGAATTGTA GAAAGAGCTCTTTCTGAGCAGATTAACATCTTCTTTGACTATAGTGGGAGAGATTTGGAAGACAAAGAA GGAGAGATTCAAGCAGGTGCTAAACTGTCATTAAATCGACAATTTTTTGACGAACGTTGGTCAAAGCAT CGGGTGGTTAGTTGTTTGGATTGGTCATCTCAGTATCCGGAGTTACTCGTGGCTTCCTATAACAACAAT GAAGATGCCCCTCATGAGCCTGATGGTGTGGCCCTTGTATGGAATATGAAATACAAAAAAACTACCCCA GAGTATGTGTTTCACTGCCAGTCAGCTGTGATGTCTGCCACATTTGCAAAATTTCATCCAAATCTTGTT GTTGGTGGTACATATTCAGGCCAAATTGTGCTTTGGGATAACCGTAGCAATAAAAGAACTCCAGTGCAA AGAACTCCACTGTCAGCAGCTGCACACACACACCCTGTATATTGTGTAAATGTTGTTGGAACACAAAAT GCTCACAATCTGATTAGCATCTCTACTGATGGAAAAATTTGTTCATGGAGTCTGGACATGCTTTCCCAT CCACAGGATAGCATGGAGTTGGTTCATAAACAGTCAAAAGCAGTAGCTGTGACATCTATGTCCTTCCCT GTTGGAGATGTCAACAACTTTGTTGTTGGGAGTGAAGAAGGTTCTGTGTACACAGCATGCCGCCATGGC AGCAAAGCTGGAATCAGTGAGATGTTTGAGGGGCATCAAGGACCAATCACTGGCATCCATTGTCATGCA GCTGTTGGAGCAGTAGACTTCTCACATCTTTTTGTCACTTCATCGTTTGACTGGACAGTAAAGCTTTGG ACAACTAAGAATAACAAGCCTTTGTATTCATTTGAAGATAATGCAGACTATGTTTATGATGTTATGTGG TCACCTACCCACCCAGCCCTGTTTGCCTGTGTGGATGGCATGGGGAGATTGGATTTGTGGAATCTCAAT AATGACACAGAGGTACCAACTGCCAGCATTTCTGTGGAGGGTAATCCTGCTCTTAATCGTGTGAGATGG ACCCATTCTGGCAGAGAGATTGCTGTGGGTGATTCTGAAGGACAGATTGTTATATACGATGTGGGAGAG CAGATTGCTGTTCCCCGCAATGATGAATGGGCACGGTTTGGCCGAACACTTGCAGAAATTAATGCAAAC CGAGCTGATGCAGAGGAGGAAGCAGCTACCCGAATACCTGCTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001271789
Insert Size	1839 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001271789.1
RefSeq Size	4319 bp
RefSeq ORF	1839 bp
Locus ID	1781
UniProt ID	Q13409
MW	68.4 kDa
Gene Summary	This gene encodes a member of the dynein intermediate chain family. The encoded protein is a non-catalytic component of the cytoplasmic dynein 1 complex, which acts as a retrograde microtubule motor to transport organelles and vesicles. A pseudogene of this gene is located on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2012] Transcript Variant: This variant (6) differs in the 5' UTR and in the coding region but maintains the reading frame, compared to variant 1. The encoded isoform (3) is shorter, compared to isoform 1. Both variants 5 and 6 encode the same isoform (3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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