CLOCK (NM_001267843) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	bHLHe8; KAT13D
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >SC332887 representing NM_001267843. Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGTTGTTTACCGTAAGCTGTAGTAAAATGAGCTCGATTGTTGACAGAGATGACAGTAGTATTTTTGAT GGGTTGGTGGAAGAAGATGACAAGGACAAAGCGAAAAGAGTATCTAGAAACAAATCTGAAAAGAAACGT AGAGATCAATTTAATGTTCTCATTAAAGAACTGGGATCCATGCTTCCTGGTAATGCTAGAAAGATGGAC AAATCTACTGTTCTGCAGAAAAGCATTGATTTTTTACGAAAACATAAAGAAATCACTGCACAGTCAGAT GCTAGTGAAATTCGACAGGACTGGAAACCTACATTCCTTAGTAATGAAGAGTTTACACAATTAATGTTA GAGGCTCTTGATGGTTTTTTTTTAGCAATCATGACAGATGGAAGCATAATATATGTGTCTGAGAGTGTA ACTTCATTACTTGAACATTTACCATCTGATCTTGTGGATCAAAGTATATTTAATTTTATCCCAGAAGGG GAACATTCAGAGGTTTATAAAATACTCTCTACTCATCTGCTGGAAAGTGATTCATTAACCCCAGAATAT TTAAAATCAAAAAATCAGTTAGAATTCTGTTGTCACATGCTGCGAGGAACAATAGACCCAAAGGAGCCA TCTACCTATGAATATGTAAAATTTATAGGAAATTTCAAATCTTTAAACAGTGTATCCTCTTCAGCACAC AATGGTTTTGAAGGAACTATACAACGCACACATAGGCCATCTTATGAAGATAGAGTTTGTTTTGTAGCT ACTGTCAGGTTAGCTACACCTCAGTTCATCAAGGAAATGTGCACTGTTGAAGAACCCAATGAAGAGTTT ACATCTAGACATAGTTTAGAATGGAAGTTTCTGTTTCTAGATCACAGGGCACCACCCATAATAGGGTAT TTGCCATTTGAAGTTCTGGGAACATCAGGCTATGATTACTATCATGTGGATGACCTAGAAAATTTGGCA AAATGTCATGAGCACTTAATGCAATATGGGAAAGGCAAATCATGTTATTATAGGTTCCTGACTAAGGGG CAACAGTGGATTTGGCTTCAGACTCATTATTATATCACTTACCATCAGTGGAATTCAAGGCCAGAGTTT ATTGTTTGTACTCACACTGTAGTAAGTTATGCAGAAGTTAGGGCTGAAAGACGACGAGAACTTGGCATT GAAGAGTCTCTTCCTGAGACAGCTGCTGACAAAAGCCAAGATTCTGGGTCAGATAATCGTATAAACACA GTCAGTCTCAAGGAAGCATTGGAAAGGTTTGATCACAGCCCAACCCCTTCTGCCTCTTCTCGGAGTTCA AGAAAATCATCTCACACGGCCGTCTCAGACCCTTCCTCAACACCAACCAAGATCCCGACGGATACGAGC ACTCCACCCAGGCAGCATTTACCAGCTCATGAGAAGATGGTGCAAAGAAGGTCATCATTTAGTAGTCAG TCCATAAATTCCCAGTCTGTTGGTTCATCATTAACACAGCCAGTGATGTCTCAAGCTACAAATTTACCA ATTCCACAAGGCATGTCCCAGTTTCAGTTTTCAGCTCAATTAGGAGCCATGCAACATCTGAAAGACCAA TTGGAACAACGGACACGCATGATAGAAGCAAATATTCATCGGCAACAAGAAGAACTAAGAAAAATTCAA GAACAACTTCAGATGGTCCATGGTCAGGGGCTGCAGATGTTTTTGCAACAATCAAATCCTGGGTTGAAT TTTGGTTCCGTTCAACTTTCTTCTGGAAATTCATCTAATATCCAGCAACTTGCACCTATAAATATGCAA GGCCAAGTTGTTCCTACTAACCAGATTCAAAGTGGAATGAATACTGGACACATTGGCACAACTCAGCAC ATGATACAACAACAGACTTTACAGAGTACATCAACTCAGAGTCAACAAAATGTACTGAGTGGGCACAGT CAGCAAACATCTCTACCCAGTCAGACACAGAGCACTCTTACAGCCCCACTGTATAACACTATGGTGATT TCTCAGCCTGCAGCCGGAAGCATGGTCCAGATTCCATCTAGTATGCCACAAAACAGCACCCAGAGTGCT GCAGTAACTACATTCACTCAGGACAGGCAGATAAGATTTTCTCAAGGTCAACAACTTGTGACCAAATTA GTGACTGCTCCTGTAGCTTGTGGGGCAGTCATGGTACCTAGTACTATGCTTATGGGCCAGGTGGTGACT GCATATCCTACTTTTGCTACACAACAGCAACAGTCACAGACATTGTCAGTAACGCAGCAGCAGCAGCAG CAGAGCTCCCAGGAGCAGCAGCTCACTTCAGTTCAGCAACCATCTCAGGCTCAGCTGACCCAGCCACCG CAACAATTTTTACAGACTTCTAGGTTGCTCCATGGGAATCCCTCAACTCAACTCATTCTCTCTGCTGCA TTTCCTCTACAACAGAGCACCTTCCCTCAGTCACATCACCAGCAACATCAGTCTCAGCAACAGCAGCAA CTCAGCCGGCACAGGACTGACAGCTTGCCCGACCCTTCCAAGGTTCAACCACAGTAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
Restriction Sites	SgfI-MluI
ACCN	NM_001267843
Insert Size	2541 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001267843.1
RefSeq Size	10981 bp
RefSeq ORF	2541 bp
Locus ID	9575
UniProt ID	O15516
Protein Families	Druggable Genome, Transcription Factors
Protein Pathways	Circadian rhythm - mammal
MW	95.3 kDa
Gene Summary	The protein encoded by this gene plays a central role in the regulation of circadian rhythms. The protein encodes a transcription factor of the basic helix-loop-helix (bHLH) family and contains DNA binding histone acetyltransferase activity. The encoded protein forms a heterodimer with ARNTL (BMAL1) that binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Polymorphisms in this gene may be associated with behavioral changes in certain populations and with obesity and metabolic syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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