NKCC1 (SLC12A2) (NM_001256461) Human Untagged Clone
CAT#: SC332389
SLC12A2 (untagged) - Homo sapiens solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant 2
CNY 11,590.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | BSC; BSC2; KILQS; NKCC1; PPP1R141 |
Vector | pCMV6-Entry |
Sequence Data |
>SC332389 representing NM_001256461.
Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGGAGCCGCGGCCCACGGCGCCCTCCTCCGGCGCCCCGGGACTGGCCGGGGTCGGGGAGACGCCGTCA GCCGCTGCGCTGGCCGCAGCCAGGGTGGAACTGCCCGGCACGGCTGTGCCCTCGGTGCCGGAGGATGCT GCGCCCGCGAGCCGGGACGGCGGCGGGGTCCGCGATGAGGGCCCCGCGGCGGCCGGGGACGGGCTGGGC AGACCCTTGGGGCCCACCCCGAGCCAGAGCCGTTTCCAGGTGGACCTGGTTTCCGAGAACGCCGGGCGG GCCGCTGCTGCGGCGGCGGCGGCGGCGGCGGCAGCGGCGGCGGCTGGTGCTGGGGCGGGGGCCAAGCAG ACCCCCGCGGACGGGGAAGCCAGCGGCGAGAGCGAGCCGGCTAAAGGCAGCGAGGAAGCCAAGGGCCGC TTCCGCGTGAACTTCGTGGACCCAGCTGCCTCCTCGTCGGCTGAAGACAGCCTGTCAGATGCTGCCGGG GTCGGAGTCGACGGGCCCAACGTGAGCTTCCAGAACGGCGGGGACACGGTGCTGAGCGAGGGCAGCAGC CTGCACTCCGGCGGCGGCGGCGGCAGTGGGCACCACCAGCACTACTATTATGATACCCACACCAACACC TACTACCTGCGCACCTTCGGCCACAACACCATGGACGCTGTGCCCAGGATCGATCACTACCGGCACACA GCCGCGCAGCTGGGCGAGAAGCTGCTCCGGCCTAGCCTGGCGGAGCTCCACGACGAGCTGGAAAAGGAA CCTTTTGAGGATGGCTTTGCAAATGGGGAAGAAAGTACTCCAACCAGAGATGCTGTGGTCACGTATACT GCAGAAAGTAAAGGAGTCGTGAAGTTTGGCTGGATCAAGGGTGTATTAGTACGTTGTATGTTAAACATT TGGGGTGTGATGCTTTTCATTAGATTGTCATGGATTGTGGGTCAAGCTGGAATAGGTCTATCAGTCCTT GTAATAATGATGGCCACTGTTGTGACAACTATCACAGGATTGTCTACTTCAGCAATAGCAACTAATGGA TTTGTAAGAGGAGGAGGAGCATATTATTTAATATCTAGAAGTCTAGGGCCAGAATTTGGTGGTGCAATT GGTCTAATCTTCGCCTTTGCCAACGCTGTTGCAGTTGCTATGTATGTGGTTGGATTTGCAGAAACCGTG GTGGAGTTGCTTAAGGAACATTCCATACTTATGATAGATGAAATCAATGATATCCGAATTATTGGAGCC ATTACAGTCGTGATTCTTTTAGGTATCTCAGTAGCTGGAATGGAGTGGGAAGCAAAAGCTCAGATTGTT CTTTTGGTGATCCTACTTCTTGCTATTGGTGATTTCGTCATAGGAACATTTATCCCACTGGAGAGCAAG AAGCCAAAAGGGTTTTTTGGTTATAAATCTGAAATATTTAATGAGAACTTTGGGCCCGATTTTCGAGAG GAAGAGACTTTCTTTTCTGTATTTGCCATCTTTTTTCCTGCTGCAACTGGTATTCTGGCTGGAGCAAAT ATCTCAGGTGATCTTGCAGATCCTCAGTCAGCCATACCCAAAGGAACACTCCTAGCCATTTTAATTACT ACATTGGTTTACGTAGGAATTGCAGTATCTGTAGGTTCTTGTGTTGTTCGAGATGCCACTGGAAACGTT AATGACACTATCGTAACAGAGCTAACAAACTGTACTTCTGCAGCCTGCAAATTAAACTTTGATTTTTCA TCTTGTGAAAGCAGTCCTTGTTCCTATGGCCTAATGAACAACTTCCAGGTAATGAGTATGGTGTCAGGA TTTACACCACTAATTTCTGCAGGTATATTTTCAGCCACTCTTTCTTCAGCATTAGCATCCCTAGTGAGT GCTCCCAAAATATTTCAGGCTCTATGTAAGGACAACATCTACCCAGCTTTCCAGATGTTTGCTAAAGGT TATGGGAAAAATAATGAACCTCTTCGTGGCTACATCTTAACATTCTTAATTGCACTTGGATTCATCTTA ATTGCTGAACTGAATGTTATTGCACCAATTATCTCAAACTTCTTCCTTGCATCATATGCATTGATCAAT TTTTCAGTATTCCATGCATCACTTGCAAAATCTCCAGGATGGCGTCCTGCATTCAAATACTACAACATG TGGATATCACTTCTTGGAGCAATTCTTTGTTGCATAGTAATGTTCGTCATTAACTGGTGGGCTGCATTG CTAACATATGTGATAGTCCTTGGGCTGTATATTTATGTTACCTACAAAAAACCAGATGTGAATTGGGGA TCCTCTACACAAGCCCTGACTTACCTGAATGCACTGCAGCATTCAATTCGTCTTTCTGGAGTGGAAGAC CACGTGAAAAACTTTAGGCCACAGTGTCTTGTTATGACAGGTGCTCCAAACTCACGTCCAGCTTTACTT CATCTTGTTCATGATTTCACAAAAAATGTTGGTTTGATGATCTGTGGCCATGTACATATGGGTCCTCGA AGACAAGCCATGAAAGAGATGTCCATCGATCAAGCCAAATATCAGCGATGGCTTATTAAGAACAAAATG AAGGCATTTTATGCTCCAGTACATGCAGATGACTTGAGAGAAGGTGCACAGTATTTGATGCAGGCTGCT GGTCTTGGTCGTATGAAGCCAAACACACTTGTCCTTGGATTTAAGAAAGATTGGTTGCAAGCAGATATG AGGGATGTGGATATGTATATAAACTTATTTCATGATGCTTTTGACATACAATATGGAGTAGTGGTTATT CGCCTAAAAGAAGGTCTGGATATATCTCATCTTCAAGGACAAGAAGAATTATTGTCATCACAAGAGAAA TCTCCTGGCACCAAGGATGTGGTAGTAAGTGTGGAATATAGTAAAAAGTCCGATTTAGATACTTCCAAA CCACTCAGTGAAAAACCAATTACACACAAAGAATCCAAAGGCCCTATTGTGCCTTTAAATGTAGCTGAC CAAAAGCTTCTTGAAGCTAGTACACAGTTTCAGAAAAAACAAGGAAAGAATACTATTGATGTCTGGTGG CTTTTTGATGATGGAGGTTTGACCTTATTGATACCTTACCTTCTGACGACCAAGAAAAAATGGAAAGAC TGTAAGATCAGAGTATTCATTGGTGGAAAGATAAACAGAATAGACCATGACCGGAGAGCGATGGCTACT TTGCTTAGCAAGTTCCGGATAGACTTTTCTGATATCATGGTTCTAGGAGATATCAATACCAAACCAAAG AAAGAAAATATTATAGCTTTTGAGGAAATCATTGAGCCATACAGACTTCATGAAGATGATAAAGAGCAA GATATTGCAGATAAAATGAAAGAAGATGAACCATGGCGAATAACAGATAATGAGCTTGAACTTTATAAG ACCAAGACATACCGGCAGATCAGGTTAAATGAGTTATTAAAGGAACATTCAAGCACAGCTAATATTATT GTCATGAGTCTCCCAGTTGCACGAAAAGGTGCTGTGTCTAGTGCTCTCTACATGGCATGGTTAGAAGCT CTATCTAAGGACCTACCACCAATCCTCCTAGTTCGTGGGAATCATCAGAGTGTCCTTACCTTCTATTCA TAA |
Restriction Sites | SgfI-MluI |
ACCN | NM_001256461 |
Insert Size | 3591 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001256461.1 |
RefSeq Size | 6843 bp |
RefSeq ORF | 3591 bp |
Locus ID | 6558 |
Protein Families | Druggable Genome, Transmembrane |
Protein Pathways | Vibrio cholerae infection |
MW | 129.7 kDa |
Gene Summary | The protein encoded by this gene mediates sodium and chloride transport and reabsorption. The encoded protein is a membrane protein and is important in maintaining proper ionic balance and cell volume. This protein is phosphorylated in response to DNA damage. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jan 2012] Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC235211 | SLC12A2 (Myc-DDK tagged) - Homo sapiens solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant 2 |
CNY 11,970.00 |
|
RG235211 | SLC12A2 (tGFP-tagged) - Homo sapiens solute carrier family 12 (sodium/potassium/chloride transporter), member 2 (SLC12A2), transcript variant 2 |
CNY 13,210.00 |