alpha Adaptin (AP2A2) (NM_001242837) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	ADTAB; CLAPA2; HIP-9; HIP9; HYPJ
Vector	pCMV6-Entry
Sequence Data	Fully Sequenced ORF (showhide) >SC331895 representing NM_001242837. Blue=Insert sequence Red=Cloning site Green=Tag(s) ATGCCGGCCGTGTCCAAGGGGGACGGGATGCGGGGCCTGGCGGTCTTCATCTCGGATATCCGCAACTGT AAAAGTAAAGAAGCAGAAATAAAAAGGATAAACAAGGAACTGGCAAATATCAGATCAAAATTTAAAGGT GACAAGGCTCTTGATGGCTATAGTAAAAAAAAGTACGTCTGCAAGTTGCTCTTCATCTTTCTCCTTGGT CATGACATTGACTTTGGACACATGGAGGCTGTGAACCTGCTGAGTTCAAACAGATACACGGAAAAGCAG ATCGGCTACCTTTTCATCTCTGTGTTGGTGAACTCAAACAGTGAGCTGATCCGCCTGATCAACAACGCC ATCAAGAATGACCTGGCCAGCCGCAACCCCACCTTCATGGGCCTGGCCCTGCACTGCATCGCCAGCGTG GGCAGCCGGGAGATGGCCGAGGCCTTCGCCGGGGAGATCCCTAAGGTCCTCGTAGCCGGAGACACTATG GACAGCGTGAAGCAGAGCGCGGCCCTGTGCTTGCTGCGCCTGTACAGGACGTCCCCCGATCTTGTCCCC ATGGGCGACTGGACATCCCGAGTGGTGCACCTGCTCAATGACCAGCACTTGGGTGTGGTAACTGCAGCC ACAAGTCTGATCACCACTTTAGCACAGAAGAACCCAGAAGAGTTTAAAACCTCCGTGTCTCTGGCTGTC TCTAGGCTAAGCAGAATCGTGACGTCTGCATCCACAGATCTCCAGGATTACACTTACTATTTTGTCCCG GCTCCCTGGCTGTCTGTCAAACTGCTGAGACTGCTGCAGTGCTACCCACCCCCAGAAGACCCTGCAGTG CGAGGCCGCCTGACTGAGTGCCTGGAGACCATCCTGAACAAAGCCCAAGAACCGCCCAAGTCGAAGAAG GTCCAGCACTCCAACGCGAAGAATGCCGTGCTCTTCGAGGCCATCAGCTTAATCATTCACCATGACAGT GAGCCGAACCTGCTCGTCCGTGCCTGCAACCAGTTGGGCCAGTTTCTGCAGCACCGCGAGACCAACCTG CGCTACCTGGCCCTGGAGAGCATGTGCACGCTGGCCAGCTCTGAGTTCTCCCATGAGGCTGTCAAGACG CACATCGAGACGGTCATCAACGCCCTGAAGACTGAGCGGGACGTGAGCGTGCGGCAGCGGGCCGTGGAC CTCCTCTACGCCATGTGCGACCGCAGCAACGCCCCACAGATCGTGGCCGAGATGCTGAGCTATCTGGAG ACAGCTGACTACTCCATCCGAGAAGAGATTGTGCTGAAGGTCGCCATCCTGGCTGAGAAGTACGCGGTG GACTACACCTGGTATGTGGATACCATCTTGAACTTGATCCGAATTGCTGGTGATTACGTGAGTGAAGAG GTGTGGTACCGAGTCATTCAGATCGTCATCAACCGGGACGACGTGCAGGGCTACGCGGCCAAGACTGTG TTCGAGGCTCTTCAGGCTCCCGCGTGCCACGAGAACCTGGTCAAAGTGGGCGGCTACATCCTGGGGGAG TTTGGAAACTTGATAGCTGGAGACCCGAGATCCAGCCCGCTGATCCAGTTCCACCTGCTGCACTCCAAG TTCCACCTGTGCAGCGTCCCCACCCGCGCGCTGCTCCTGTCCACCTACATCAAGTTCGTGAACCTCTTC CCGGAGGTGAAGCCCACCATCCAGGACGTGCTGCGCAGCGACAGCCAGCTCAGGAACGCAGACGTGGAG CTGCAGCAGCGTGCTGTGGAGTACCTGCGGCTCAGCACCGTGGCCAGCACCGACATTCTGGCGACCGTG CTGGAGGAGATGCCCCCATTCCCGGAGCGGGAGTCCTCCATCTTGGCAAAGCTCAAGAAGAAGAAGGGC CCCAGCACGGTGACAGACCTGGAGGACACCAAGCGGGACAGGAGTGTGGACGTGAACGGGGGTCCTGAG CCTGCCCCAGCCAGTACCAGCGCCGTGTCTACGCCTTCTCCGTCGGCAGACCTGCTGGGTCTCGGGGCT GCCCCCCCTGCCCCCGCGGGCCCCCCACCCTCCTCCGGCGGCAGCGGGCTGCTCGTGGACGTGTTCTCA GACTCGGCCTCTGTGGTCGCGCCTCTCGCTCCTGGCTCCGAAGACAACTTTGCCAGGTTTGTTTGTAAA AACAATGGTGTGTTGTTTGAAAACCAGCTGCTTCAAATTGGACTTAAGTCTGAATTTCGGCAGAATTTA GGTCGGATGTTTATCTTTTATGGTAATAAGACCTCCACGCAGTTCCTAAACTTTACCCCAACACTAATC TGTTCAGACGACCTTCAGCCTAACCTGAACCTGCAGACCAAGCCCGTGGACCCGACCGTGGAGGGGGGC GCGCAGGTGCAGCAGGTGGTCAACATAGAGTGCGTGTCCGACTTCACGGAGGCGCCAGTCCTCAACATT CAGTTCAGGTATGGGGGCACCTTCCAGAACGTGTCTGTGCAGCTGCCCATCACTCTCAACAAATTCTTC CAGCCGACAGAAATGGCTTCTCAGGATTTCTTTCAACGTTGGAAGCAGTTGAGCAATCCACAGCAGGAA GTGCAGAACATCTTCAAAGCAAAGCACCCAATGGACACAGAAGTCACCAAAGCCAAGATCATTGGATTT GGTTCTGCACTTCTTGAAGAAGTTGATCCTAATCCTGCGAATTTCGTGGGAGCTGGAATCATCCACACG AAAACCACCCAGATTGGATGCCTGCTGCGCTTGGAGCCGAACCTGCAAGCCCAGATGTACCGGCTCACG CTGCGCACAAGTAAGGAAGCCGTTTCTCAGAGATTATGTGAATTGCTCTCAGCGCAGTTTTAG
Restriction Sites	SgfI-MluI
ACCN	NM_001242837
Insert Size	2823 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001242837.1
RefSeq Size	4674 bp
RefSeq ORF	2823 bp
Locus ID	161
UniProt ID	O94973
Protein Pathways	Endocytosis, Huntington's disease
MW	104.1 kDa
Gene Summary	The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane. [provided by RefSeq, Nov 2016] Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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