Fibrinogen beta chain (FGB) (NM_001184741) Human Untagged Clone

CAT#: SC328711

FGB (untagged)-Human fibrinogen beta chain (FGB) transcript variant 2



  "NM_001184741" in other vectors (4)

CNY 6,940.00


货期*
4周

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-FGB Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HEL-S-78p
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>SC328711 representing NM_001184741.
Blue=Insert sequence Red=Cloning site Green=Tag(s)

GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
ATGAAAAGGATGGTTTCTTGGAGCTTCCACAAACTTAAAACCATGAAACATCTATTATTGCTACTATTG
TGTGTTTTTCTAGTTAAGTCCCAAGGTGTCAACGACAATGAGGAGGGTTTCTTCAGTGCCCGTGGTCAT
CGACCCCTTGACAAGAAGAGAGAAGAGGCTTTGCTACAACAGGAAAGGCCAATCAGAAATAGTGTTGAT
GAGTTAAATAACAATGTGGAAGCTGTTTCCCAGACCTCCTCTTCTTCCTTTCAGTACATGTATTTGCTG
AAAGACCTGTGGCAAAAGAGGCAGAAGCAAGTAAAAGATAATGAAAATGTAGTCAATGAGTACTCCTCA
GAACTGGAAAAGCACCAATTATATATAGATGAGACTGTGAATAGCAATATCCCAACTAACCTTCGTGTG
CTTCGTTCAATCCTGGAAAACCTGAGAAGCAAAATACAAAAGTTAGAATCTGATGTCTCAGCTCAAATG
GAATATTGTCGCACCCCATGCACTGTCAGTTGCAATATTCCTGTGGTGTCTGGCAAAGAATGTGAGGAA
ATTATCAGGAAAGGAGGTGAAACATCTGAAATGTATCTCATTCAACCTGACAGTTCTGTCAAACCGTAT
AGAGTATACTGTGACATGAATACAGAAAATGGAGGATGGACAGTGATTCAGAACCGTCAAGACGGTAGT
GTTGACTTTGGCAGGAAATGGGATCCATATAAACAGGGATTTGGAAATGTTGCAACCAACACAGATGGG
AAGAATTACTGTGGCCTACCAGGTGAATATTGGCTTGGAAATGATAAAATTAGCCAGCTTACCAGGATG
GGACCCACAGAACTTTTGATAGAAATGGAGGACTGGAAAGGAGACAAAGTAAAGGCTCACTATGGAGGA
TTCACTGTACAGAATGAAGCCAACAAATACCAGATCTCAGTGAACAAATACAGAGGAACAGCCGGTAAT
GCCCTCATGGATGGAGCATCTCAGCTGATGGGAGAAAACAGGACCATGACCATTCACAACGGCATGTTC
TTCAGCACGTATGACAGAGACAATGACGGCTGGTTAACATCAGATCCCAGAAAACAGTGTTCTAAAGAA
GACGGTGGTGGATGGTGGTATAATAGATGTCATGCAGCCAATCCAAACGGCAGATACTACTGGGGTGGA
CAGTACACCTGGGACATGGCAAAGCATGGCACAGATGATGGTGTAGTATGGATGAATTGGAAGGGGTCA
TGGTACTCAATGAGGAAGATGAGTATGAAGATCAGGCCCTTCTTCCCACAGCAATAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
TACAAGGATGACGACGATAAG
GTTTAAACGGCCGGC
Restriction Sites SgfI-MluI     
ACCN NM_001184741
Insert Size 1299 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001184741.1
RefSeq Size 3451 bp
RefSeq ORF 1299 bp
Locus ID 2244
UniProt ID P02675
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Complement and coagulation cascades
MW 50 kDa
Gene Summary The protein encoded by this gene is the beta component of fibrinogen, a blood-borne glycoprotein comprised of three pairs of nonidentical polypeptide chains. Following vascular injury, fibrinogen is cleaved by thrombin to form fibrin which is the most abundant component of blood clots. In addition, various cleavage products of fibrinogen and fibrin regulate cell adhesion and spreading, display vasoconstrictor and chemotactic activities, and are mitogens for several cell types. Fibrinogen serves key roles in hemostasis and antimicrobial host defense. Mutations in this gene lead to several disorders, including afibrinogenemia, dysfibrinogenemia, hypodysfibrinogenemia and thrombotic tendency. [provided by RefSeq, Aug 2020]
Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. This results in a shorter protein (isoform 2), compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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