MIER1 (NM_001146111) Human Untagged Clone

CAT#: SC327549

MIER1 (untagged)-Human mesoderm induction early response 1 homolog (Xenopus laevis) (MIER1) transcript variant 7



  "NM_001146111" in other vectors (4)

CNY 7,220.00


货期*
6周

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


MIER1 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ER1; MI-ER1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_001146111, the custom clone sequence may differ by one or more nucleotides
ATGTTTATGTTTAATTGGTTTACAGACTGTCTGTGGACTCTTTTCCTGTCAAATTACCAG
CCATCTGTTGAATCTTCAAGTCCAGGAGGTTCAGCAACATCAGATGACCATGAATTTGAT
CCATCAGCTGACATGCTGGTTCATGATTTTGATGATGAACGAACATTAGAAGAGGAAGAA
ATGATGGAAGGAGAAACAAACTTCAGCTCTGAAATAGAAGATCTTGCAAGGGAAGGCGAC
ATGCCAATTCATGAACTTCTCAGCCTTTATGGTTATGGTAGTACTGTTCGACTACCTGAA
GAAGATGAGGAAGAGGAAGAAGAGGAAGAAGAAGGTGAAGATGATGAAGATGCTGATAAT
GATGACAACAGTGGCTGTAGTGGGGAAAATAAAGAGGAGAATATAAAGGATTCATCAGGT
CAGGAGGATGAAACTCAGTCTTCCAATGATGATCCATCACAATCTGTTGCTTCTCAAGAT
GCCCAGGAAATAATCCGCCCACGTCGATGTAAATATTTTGATACAAATAGTGAAGTAGAA
GAAGAATCTGAAGAAGATGAAGATTATATTCCATCAGAAGACTGGAAAAAGGAGATTATG
GTGGGCTCCATGTTTCAAGCAGAAATTCCAGTTGGCATTTGTAGATACAAAGAAAATGAA
AAAGTATATGAAAATGATGATCAGCTCCTGTGGGACCCTGAGTACTTACCAGAAGATAAA
GTGATTATATTTCTTAAAGATGCATCTAGAAGAACAGGTGATGAGAAGGGTGTAGAAGCA
ATTCCTGAAGGATCTCACATAAAAGACAATGAACAGGCTTTATATGAATTGGTTAAATGC
AATTTTGATACAGAAGAAGCATTGAGAAGATTAAGATTTAATGTAAAAGCAGCTAGAGAG
GAATTATCTGTTTGGACAGAGGAAGAGTGTAGAAATTTTGAACAAGGGCTGAAGGCCTAT
GGAAAGGATTTTCATTTGATTCAGGCTAATAAAGTCCGAACAAGGTCAGTTGGTGAATGT
GTAGCATTCTATTACATGTGGAAAAAATCTGAACGTTATGATTTCTTTGCTCAGCAAACA
CGATTTGGAAAGAAGAAATATAATCTTCATCCTGGTGTAACGGATTACATGGATCGTCTT
CTAGACGAAAGTGAAAGTGCTGCATCTAGTCGAGCACCATCCCCTCCCCCAACTGCATCA
AACAGTAGTAACAGCCAGTCTGAGAAAGAAGATGGCACTGTAAGCACTGCTAATCAAAAT
GGAGTGTCATCTAATGGACCAGGTGAAATATTAAACAAAGAGGAAGTAAAAGTTGAAGGG
TTACACATTAATGGACCAACAGGCATACTCCAAATGCTTCTTCCAGTTCATTTTTCAGCC
ATCAGTTCAAGAGCCAATGCCTTTTTAAAA
Restriction Sites Please inquire     
ACCN NM_001146111
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001146111.1, NP_001139583.1
RefSeq Size 3893 bp
RefSeq ORF 1413 bp
Locus ID 57708
UniProt ID Q8N108
Gene Summary This gene encodes a protein that was first identified in Xenopus laevis by its role in a mesoderm induction early response (MIER). The encoded protein functions as a transcriptional regulator. Alternatively spliced transcript variants encode multiple isoforms, some of which lack a C-terminal nuclear localization signal. [provided by RefSeq, May 2013]
Transcript Variant: This variant (7) lacks an alternate segment in the 3' coding region, compared to variant 1. The resulting isoform (g) has a shorter and distinct C-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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