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ADCK1 (NM_020421) Human Untagged Clone
CAT＃: SC323351
ADCK1 (untagged)-Kinase deficient mutant (K176M) of Human aarF domain containing kinase 1 (ADCK1), transcript variant 1
CNY 5,848.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>NCBI ORF sequence for NM_020421, the custom clone sequence may differ by one or more nucleotides
ATGGCCAGAAAGGCTCTCAAGCTTGCTTCGTGGACCAGCATGGCTCTTGCTGCCTCTGGCATCTACTTCT ACAGTAACAAGTACTTGGACCCTAATGACTTTGGCGCTGTCAGGGTGGGCAGAGCAGTTGCTACGACGGC TGTCATCAGTTACGACTACCTCACTTCCCTGAAGAGTGTCCCTTATGGCTCAGAGGAGTACTTGCAGCTG AGATCTAAGGTGCACCTTCGCTCTGCCAGGCGTCTCTGTGAGCTCTGCTGTGCCAACCGGGGCACCTTCA TCAAGGTGGGCCAGCACCTGGGGGCTCTGGACTACCTGTTGCCAGAGGAGTACACCAGCACGCTGAAGGT ACTGCACAGCCAGGCTCCACAGAGCAGCATGCAAGAGATCCGCCAGGTCATCCGAGAAGATCTGGGCAAG GAGATCCATGATTTGTTCCAGAGCTTCGATGACACCCCTCTGGGGACGGCCTCCCTGGCCCAGGTCCACA AGGCAGTGCTGCATGATGGGCGGACGGTGGCCGTGAAGGTCCAGCACCCAAAGGTGCGGGCTCAGAGCTC GAAGGACATTCTCCTGATGGAGGTGCTCGTTCTGGCTGTGAAGCAGCTGTTCCCAGAGTTTGAGTTTATG TGGCTTGTGGATGAAGCCAAGAAGAACCTGCCTTTGGAGCTGGATTTCCTCAATGAAGGGAGGAATGCTG AGAAGGTGTCCCAGATGCTCAGGCATTTTGACTTCTTGAAGGTCCCCCGAATCCACTGGGACCTGTCCAC GGAGCGGGTCCTCCTGATGGAGTTTGTGGATGGCGGGCAGGTCAATGACAGAGACTACATGGAGAGGAAC AAGATCGACGTCAATGAGATCTCACGCCACCTGGGCAAGATGTATAGTGAGATGATCTTCGTCAATGGCT TCGTGCACTGCGATCCCCACCCCGGCAATGTACTGGTGCGGAAGCACCCCGGCACGGGAAAGGCGGAGAT TGTCCTGTTGGACCATGGGCTTTACCAGATGCTCACGGAAGAATTCCGCCTGAATTACTGCCACCTCTGG CAGTCTCTGATCTGGACTGACATGAAGAGAGTGAAGGAGTACAGCCAGCGACTGGGAGCCGGGGATCTCT ACCCCTTGTTTGCCTGCATGCTGACGGCGCGATCGTGGGACTCGGTCAACAGAGGCATCAGCCAAGCTCC CGTCACTGCCACTGAGGACTTAGAGATTCGCAACAACGCGGCCAACTACCTCCCCCAGATCAGCCATCTC CTCAACCACGTGCCGCGCCAGATGCTGCTCATCTTGAAGACCAACGACCTGCTGCGTGGCATTGAGGCCG CCCTGGGCACCCGCGCCAGCGCCAGCTCCTTTCTCAACATGTCACGTTGCTGCATCAGAGCGCTAGCTGA GCACAAGAAGAAGAATACCTGTTCATTCTTCAGAAGGACCCAGATCTCTTTCAGCGAGGCCTTCAACTTA TGGCAGATCAACCTCCATGAGCTCATCCTGCGTGTGAAGGGGTTGAAGCTGGCTGACCGGGTCTTGGCCC TAATATGCTGGCTGTTCCCTGCTCCACTCTGA >OriGene 5' read for mutant NM_020421 unedited
ACCGCCCGTTACAGCAACGGGCGGTAGGCGCGTACGGTGGGAGGTTCTATATAAGCAGAAGCTCGTTTAG TGAACCGTCAGAATATTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAGCCGGG CGCCGCGGCTCTGCTTCCCTCGGGGATCTGGCGACATGGCCAGAAAGGCTCTCAAGCTTGCTTCGTGGAC CAGCATGGCTCTTGCTGCCTCTGGCATCTACTTCTACAGTAACAAGTACTTGGACCCTAATGACTTTGGC GCTGTCAGGGTGGGCAGAGCAGTTGCTACGACGGGCTGTCATCAGTTACGACTACCTCACTTTCCCTGAA GAGTGTCTCCTTATGGCTCAGAAGGAGTAACTTTGCCAGGCTTGAAGAATCTCTAAGAATCCATGGATTT TGTTTCCAGAGACGCTTCGAGATGACACCCCCTCGGGGGACGCGCGCTCTCCCTTGCCCCAAGGGTCCAC CAAGGGCATGTGCCTGGCTGTAGATGGGGCGGGACGTGTGGCCGTTGATGGGTCAACACCCCCAAAGGGT GGCGGGGCTAAAAACCTCGAAGGGACTTTTCTCCTGGAAGGGAGGGCGCTCGTTTTGGCGCTGTGAAACC CACTGTTTCCCAAAATTTTGATTTATATTGGGCGTTGGGGGGAAAACCCAAAAAAAAACCGGGGCCTTGG GAACTGGGTTTCCCCAAGGAAGGGGGGAAGTGCCGAAAAGGGTTCCCAATCCCCCCGGCTTTTTACTTTT TTAAAGGGCCCCCAAACCCCGGGGCCCTGCCCCGGGGGGGGGCCCTCGGGGAGTTTTTGGGAGGGGGGGC GCGGCCTATATACAAGAACCACTCGTGAAGGAAAAACTTTATCTTCTCCTGATATTTCTCCCACCCGCGG GGAAAATATATTTAGAAAAAACTTCTCAGAGTTCTGGCTA >SC323351 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
CSATGMGCAATGGGCGGTAGGCKGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGTCA GAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGGAAGCCGGGCGCCGCGGCT CTGCTTCCCTCGGGGATCTGGCGACATGGCCAGAAAGGCTCTCAAGCTTGCTTCGTGGACCAGCATGGCT CTTGCTGCCTCTGGCATCTACTTCTACAGTAACAAGTACTTGGAC |
| Restriction Sites | Please inquire |
| ACCN | NM_020421 |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_020421.2, NP_065154.2 |
| RefSeq Size | 2222 bp |
| RefSeq ORF | 1572 bp |
| Locus ID | 57143 |
| UniProt ID | Q86TW2 |
| Domains | ABC1 |
| Protein Families | Druggable Genome, Protein Kinase, Secreted Protein |
| Gene Summary | The function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr).[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC208924 | ADCK1 (Myc-DDK-tagged)-Human aarF domain containing kinase 1 (ADCK1), transcript variant 1 |
CNY 3,990.00 |
|
| RC208924L3 | Lenti-ORF clone of ADCK1 (Myc-DDK-tagged)-Human aarF domain containing kinase 1 (ADCK1), transcript variant 1 |
CNY 5,890.00 |
|
| RC208924L4 | Lenti-ORF clone of ADCK1 (mGFP-tagged)-Human aarF domain containing kinase 1 (ADCK1), transcript variant 1 |
CNY 5,890.00 |
|
| RG208924 | ADCK1 (tGFP-tagged) - Human aarF domain containing kinase 1 (ADCK1), transcript variant 1 |
CNY 7,832.00 |
|
| SC321874 | ADCK1 (untagged)-Human aarF domain containing kinase 1 (ADCK1), transcript variant 1 |
CNY 5,848.00 |
