DUSP6 (NM_001946) Human Untagged Clone
CAT#: SC321781
DUSP6 (untagged)-Human dual specificity phosphatase 6 (DUSP6), transcript variant 1
CNY 5,488.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | HH19; MKP3; PYST1 |
Vector | pCMV6-AC |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>OriGene sequence for NM_001946.2
GCCTCGCGCAGGGGGTGTGCGAGGCTGAGTCCAAGAGATAGCAAATCGAGTCTTAAATAA
TCCGGGGAGAAAGACGCCCGGGTAGATTTGAGGTGCAGCCTTGGAGGGAGGGATTAGAAG CCGCTAGACTTTTTTTCCTCCCCTCTCAGTAGCACGGAGTCCGAATTAATTGGATTTCAT TCACTGGGGAGGAACAAAAACTATCTGGGCAGCTTCATTGAGAGAGATTCATTGACACTA AGAGCCAGCGGCTGCAGCTGGGTGCAGAGAGAACCTCCGGCTTTACTTCTGTCTCGTCTG CCCCAACCGCTAGCCTCGGCTTGGGTAAGGCGAGGCGGAATTAAACCCCGCTCCGAGAGC GGCAGCTTCGCGCGCGGTGCGCTCGGCCTATGCCTGCCCCGAGGGGCGTCTGGTAGGCAC CCCGCCCTCTCCCGCAGCTCGACCCCCATGATAGATACGCTCAGACCCGTGCCCTTCGCG TCGGAAATGGCGATCAGCAAGACGGTGGCGTGGCTCAACGAGCAGCTGGAGCTGGGCAAC GAGCGGCTGCTGCTGATGGACTGCCGGCCGCAGGAGCTATACGAGTCGTCGCACATCGAG TCGGCCATCAACGTGGCCATCCCGGGCATCATGCTGCGGCGCCTGCAGAAGGGTAACCTG CCGGTGCGCGCGCTCTTCACGCGCGGCGAGGACCGGGACCGCTTCACCCGGCGCTGTGGC ACCGACACAGTGGTGCTCTACGACGAGAGCAGCAGCGACTGGAACGAGAATACGGGCGGC GAGTCGGTGCTCGGGCTGCTGCTCAAGAAGCTCAAGGACGAGGGCTGCCGGGCGTTCTAC CTGGAAGGTGGCTTCAGTAAGTTCCAAGCCGAGTTCTCCCTGCATTGCGAGACCAATCTA GACGGCTCGTGTAGCAGCAGCTCGCCGCCGTTGCCAGTGCTGGGGCTCGGGGGCCTGCGG ATCAGCTCTGACTCTTCCTCGGACATCGAGTCTGACCTTGACCGAGACCCCAATAGTGCA ACAGACTCGGATGGTAGTCCGCTGTCCAACAGCCAGCCTTCCTTCCCAGTGGAGATCTTG CCCTTCCTCTACTTGGGCTGTGCCAAAGACTCCACCAACTTGGACGTGTTGGAGGAATTC GGCATCAAGTACATCTTGAACGTCACCCCCAATTTGCCGAATCTCTTTGAGAACGCAGGA GAGTTTAAATACAAGCAAATCCCCATCTCGGATCACTGGAGCCAAAACCTGTCCCAGTTT TTCCCTGAGGCCATTTCTTTCATAGATGAAGCCCGGGGCAAGAACTGTGGTGTCTTGGTA CATTGCTTGGCTGGCATTAGCCGCTCAGTCACTGTGACTGTGGCTTACCTTATGCAGAAG CTCAATCTGTCGATGAACGATGCCTATGACATTGTCAAAATGAAAAAATCCAACATATCC CCTAACTTCAACTTCATGGGTCAGCTGCTGGACTTCGAGAGGACGCTGGGACTCAGCAGC CCATGTGACAACAGGGTTCCAGCACAGCAGCTGTATTTTACCACCCCTTCCAACCAGAAT GTATACCAGGTGGACTCTCTGCAATCTACGTGAAAGACCCCACACCCCTCCTTGCTGGAA TGTGTCTGGCCCTTCAGCAGTTTCTCTTGGCAGCATCAGCTGGGCTGCTTTCTTTGTGTG TGGCCCCAGGTGTCAAAATGACACCAGCTGTCTGTACTAGACAAGGTTACCAAGTGCGGA ATTGGTTAATACTAACAGAGAGATTTGCTCCATTCTCTTTGGAATAACAGGACATGCTGT ATAGATACAGGCAGTAGGTTTGCTCTGTACCCATGTGTACAGCCTACCCATGCAGGGACT GGGATTCGAGGACTTCCAGGCGCATAGGGTAGAACCAAATGATAGGGTAGGAGCATGTGT TCTTTAGGGCCTTGTAAGGCTGTTTCCTTTTGCATCTGGAACTGACTATATAATTGTCTT CAATGAAGACTAATTCAATTTTGCATATAGAGGAGCCAAAGAGAGATTTCAGCTCTGTAT TTGTGGTATCAGTTTGGAAAAAAAAATCTGATACTCCATTTGATTATTGTAAATATTTGA TCTTGAATCACTTGACAGTGTTTGTTTGAATTGTGTTTGTTTTTTCCTTTGATGGGCTTA AAAGAAATTATCCAAAGGGAGAAAGAGCAGTATGCCACTTCTTAAAACAGAACAAAACAA AAAAAGAAAATTGTGCTCTTTTCTAATCCAAAGGGTATATTTGCAGCATGCTTGACTTTA CCAATTCTGATGACATCTTTACGGACACTATTATCACTAAGACCTTGTTATGGCGAAGTC TTTAGTCTTTTTCATGTATTTTCCTCATGATTTTTTCTCTTTATGTAGTTTGACTATGCC TTACCTTTGTAAATATTTTTGCTTGTGTTGTCGCAAAAGGGATAATCTGGGAAAGACACC AAATCATGGGCTCACTTTAAAAAAAGAAAGAATAAAAAAACCTTCAGCTGTGCTAAACAG TATATTACCTCTGTATAAAATTCTTCAGGGAGTGTCACCTCAAATGCAATACTTTGGGTT GGTTTCTTTCCTTTAAAAAAATTTGTATAAAACTGGAAGTGTGTGTGTGTGAGCATGGGT ACCCATTTGATAAGAGAAATGCATTTGATTGTGAAGAAGGGAGAGTTAAATTCTCCATTA TGTTCGTGGTGTAAAGTTTAGAGCTGGAATTTATTATAAGAATGTAAAACCTTAAATTAT TAATAAATAACTATTTTGGCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA |
Restriction Sites | Please inquire |
ACCN | NM_001946 |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_001946.2, NP_001937.2 |
RefSeq Size | 2842 bp |
RefSeq ORF | 1146 bp |
Locus ID | 1848 |
UniProt ID | Q16828 |
Domains | DSPc, RHOD |
Protein Families | Druggable Genome, Phosphatase |
Protein Pathways | MAPK signaling pathway |
Gene Summary | The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014] Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC206765 | DUSP6 (Myc-DDK-tagged)-Human dual specificity phosphatase 6 (DUSP6), transcript variant 1 |
CNY 5,488.00 |
|
RC206765L1 | Lenti ORF clone of Human dual specificity phosphatase 6 (DUSP6), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
RC206765L2 | Lenti ORF clone of Human dual specificity phosphatase 6 (DUSP6), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
RC206765L3 | Lenti ORF clone of Human dual specificity phosphatase 6 (DUSP6), transcript variant 1, Myc-DDK-tagged |
CNY 7,888.00 |
|
RC206765L4 | Lenti ORF clone of Human dual specificity phosphatase 6 (DUSP6), transcript variant 1, mGFP tagged |
CNY 7,888.00 |
|
RG206765 | DUSP6 (tGFP-tagged) - Human dual specificity phosphatase 6 (DUSP6), transcript variant 1 |
CNY 7,088.00 |