NFAT5 (NM_173214) Human Untagged Clone

CAT#: SC309213

NFAT5 (untagged)-Human nuclear factor of activated T-cells 5, tonicity-responsive (NFAT5), transcript variant 4



  "NM_173214" in other vectors (4)

CNY 10,450.00


货期*
3周

规格
    • 10 ug

Product images

经常一起买 (3)
Anti-NFAT5 Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms NF-AT5; NFATL1; NFATZ; OREBP; TONEBP
Vector pCMV6 series
Sequence Data
>NCBI ORF sequence for NM_173214, the custom clone sequence may differ by one or more nucleotides
ATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCA
GTCACCGACAGCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAAC
AGAGGGGTAAGTGAAAAGCAGTTAACCAGTAACACAGTTCAGCAGCATCCATCAACACCG
AAGAGGCACACAGTCTTGTACATCTCACCACCACCTGAGGACTTGCTGGATAACAGTCGG
ATGTCCTGCCAGGATGAGGGGTGTGGATTGGAATCTGAGCAGAGCTGCAGTATGTGGATG
GAGGATTCCCCCTCCAACTTCAGTAACATGAGCACCAGTTCCTACAATGATAACACTGAG
GTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGACGAGAT
TGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTG
CTTTCTCAGCTTACCACGGACAACAAAGGCAACTCAAAAGCGGGAAATGGAACATTGGAA
AACCAAAAAGGAACTGGAGTAAAGAAGAGCCCTATGTTGTGTGGACAATATCCTGTTAAA
AGTGAGGGAAAGGAGCTGAAGATAGTTGTACAACCTGAGACACAGCACCGAGCTCGGTAC
CTGACTGAGGGCAGCCGTGGCTCAGTGAAAGATAGAACACAGCAAGGCTTTCCTACAGTA
AAGCTGGAAGGCCATAATGAACCTGTAGTGTTGCAAGTGTTTGTGGGCAACGACTCTGGA
CGAGTGAAACCACATGGATTTTATCAGGCCTGCAGAGTAACTGGACGAAATACAACTCCT
TGCAAAGAAGTGGACATTGAAGGCACTACTGTTATAGAAGTCGGCCTTGATCCTAGCAAC
AACATGACACTGGCGGTGGACTGCGTAGGGATATTGAAATTGAGGAATGCTGATGTCGAA
GCCAGAATAGGAATTGCTGGTTCCAAGAAGAAAAGCACTCGTGCCAGATTGGTTTTTCGA
GTTAATATCATGAGGAAAGATGGCTCCACTTTGACACTGCAAACACCCTCTTCTCCAATT
TTGTGTACTCAGCCAGCAGGAGTGCCAGAAATCTTAAAGAAAAGCTTGCATAGCTGTTCA
GTGAAAGGAGAAGAAGAAGTGTTTTTAATCGGCAAGAACTTTCTGAAAGGAACTAAAGTT
ATTTTCCAAGAAAATGTTTCTGATGAAAACTCTTGGAAGTCAGAAGCTGAAATTGATATG
GAACTATTTCATCAGAATCATCTTATTGTGAAGGTTCCTCCCTATCATGACCAACATATA
ACTTTGCCTGTGTCAGTGGGAATATATGTAGTGACAAATGCTGGAAGATCTCATGATGTT
CAACCATTCACTTACACTCCAGACCCAGCAGCAGCTGGTGCTTTGAATGTAAATGTGAAG
AAGGAAATATCTAGTCCAGCAAGACCTTGCTCTTTTGAAGAGGCCATGAAAGCAATGAAA
ACTACTGGATGTAATTTAGATAAGGTAAATATTATCCCTAATGCCCTGATGACTCCACTC
ATACCAAGCAGTATGATTAAGAGTGAAGATGTTACTCCAATGGAAGTAACAGCAGAAAAA
AGATCTTCCACTATTTTTAAGACTACAAAGTCTGTTGGATCAACTCAGCAAACATTAGAA
AACATCTCAAACATAGCAGGAAATGGCTCTTTTTCATCACCATCATCTTCCCACCTACCT
TCTGAAAATGAAAAACAGCAGCAGATTCAGCCCAAGGCATACAACCCAGAGACCCTGACA
ACTATTCAAACCCAGGACATCTCACAGCCTGGTACTTTTCCAGCAGTTTCTGCTTCTAGT
CAGCTGCCCAACAGCGATGCACTATTGCAGCAGGCTACACAGTTTCAGACAAGAGAAACT
CAGTCTAGAGAGATATTACAGTCAGATGGTACAGTGGTTAATTTGTCACAACTGACTGAG
GCATCACAACAACAGCAGCAGTCACCACTACAAGAACAAGCACAGACTTTACAGCAGCAG
ATTTCATCAAATATTTTTCCATCACCAAATAGTGTGAGTCAGCTTCAGAATACTATTCAG
CAGCTGCAAGCAGGGAGTTTCACAGGCAGTACTGCTAGTGGCAGCAGTGGAAGTGTTGAC
TTGGTCCAACAAGTTTTAGAGGCACAGCAGCAGTTATCTTCAGTTTTATTTTCTGCTCCA
GATGGTAATGAGAATGTTCAAGAGCAGCTTAGTGCAGATATTTTTCAACAAGTCAGTCAA
ATTCAGAGTGGTGTAAGCCCTGGAATGTTTTCCTCAACAGAGCCAACAGTCCATACCAGA
CCAGATAATTTATTACCTGGAAGAGCTGAAAGTGTTCATCCACAGTCTGAAAACACGTTA
TCTAATCAACAGCAGCAGCAGCAGCAGCAACAGCAAGTGATGGAATCTTCAGCCGCAATG
GTGATGGAGATGCAACAGAGTATCTGCCAGGCAGCTGCCCAGATTCAGTCAGAGTTATTC
CCTTCAACTGCTTCAGCAAATGGAAACCTTCAGCAATCGCCAGTTTACCAGCAGACTTCT
CACATGATGAGTGCATTGTCTACCAATGAGGATATGCAAATGCAGTGTGAATTGTTTTCT
TCTCCTCCTGCAGTTTCTGGAAATGAAACTTCTACAACTACCACACAGCAGGTTGCAACC
CCTGGCACTACCATGTTTCAGACATCAAGTTCAGGAGATGGAGAAGAAACTGGAACACAA
GCAAAACAGATTCAGAACAGTGTCTTTCAGACCATGGTCCAAATGCAACATAGTGGGGAC
AATCAACCTCAAGTTAACCTTTTTTCATCCACAAAAAGTATGATGAGTGTTCAGAATAGT
GGTACCCAACAACAAGGTAATGGTTTATTCCAGCAAGGGAATGAGATGATGTCACTTCAA
TCTGGAAATTTTTTGCAGCAGTCTTCTCATTCACAGGCCCAACTTTTTCATCCTCAAAAT
CCTATTGCCGATGCTCAGAACCTTTCCCAGGAAACTCAAGGTTCTCTCTTTCATAGTCCA
AATCCTATTGTCCACAGTCAGACTTCTACAACCTCCTCTGAACAAATGCAGCCTCCAATG
TTTCACTCTCAAAGTACCATTGCTGTGTTACAGGGCTCTTCAGTTCCTCAAGACCAGCAG
TCAACCAACATATTTCTTTCCCAGAGTCCCATGAATAATCTTCAGACTAACACAGTAGCC
CAAGAAGCATTTTTTGCAGCACCGAACTCAATTTCTCCACTTCAGTCAACATCAAACAGT
GAACAACAAGCTGCTTTCCAACAGCAAGCTCCAATATCACACATCCAGACTCCTATGCTT
TCCCAAGAACAGGCACAACCCCCGCAGCAGGGTTTATTTCAGCCTCAGGTGGCCCTGGGC
TCCCTTCCACCTAATCCAATGCCTCAAAGCCAACAAGGAACCATGTTCCAGTCACAGCAC
TCAATAGTTGCCATGCAGAGTAACTCTCCATCCCAGGAACAGCAGCAGCAGCAGCAACAG
CAGCAGCAACAGCAGCAGCAACAACAACAGAGCATTTTATTCAGTAATCAGAATACCATG
GCTACAATGGCGTCTCCAAAGCAACCACCACCAAACATGATATTCAACCCAAATCAAAAT
CCAATGGCTAATCAGGAGCAACAGAACCAGTCAATTTTTCACCAACAAAGTAACATGGCC
CCAATGAATCAAGAGCAACAGCCCATGCAATTTCAGAGTCAGTCCACAGTTTCCTCACTT
CAGAACCCAGGTCCTACCCAGTCGGAATCATCACAGACCCCCTTGTTCCATAGCTCTCCT
CAGATTCAGTTGGTACAAGGGTCACCTAGTTCTCAAGAGCAGCAAGTAACTCTCTTCTTA
TCTCCAGCATCCATGTCTGCCTTGCAGACCAGTATAAATCAACAAGATATGCAACAGTCT
CCTCTTTATTCCCCTCAGAACAACATGCCTGGAATTCAAGGAGCCACATCTTCGCCTCAA
CCACAGGCTACTTTATTTCACAACACAGCAGGAGGCACAATGAACCAACTGCAGAATTCT
CCTGGCTCATCTCAGCAGACATCAGGAATGTTCTTATTTGGCATTCAAAATAACTGTAGT
CAGCTTTTAACCTCTGGACCAGCTACATTGCCTGATCAGTTGATGGCCATAAGTCAGCCA
GGCCAACCACAAAACGAGGGCCAGCCACCTGTGACAACACTTCTTTCTCAGCAAATGCCA
GAGAATTCTCCACTGGCATCCTCTATAAACACCAACCAGAACATCGAAAAGATTGATTTG
CTTGTTTCATTGCAAAACCAAGGGAACAACTTGACTGGCTCCTTTTAA
Restriction Sites Please inquire     
ACCN NM_173214
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_173214.1, NP_775321.1
RefSeq Size 14185 bp
RefSeq ORF 4368 bp
Locus ID 10725
UniProt ID O94916
Protein Families Druggable Genome, Transcription Factors
Protein Pathways Axon guidance, B cell receptor signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
Gene Summary The product of this gene is a member of the nuclear factors of activated T cells family of transcription factors. Proteins belonging to this family play a central role in inducible gene transcription during the immune response. This protein regulates gene expression induced by osmotic stress in mammalian cells. Unlike monomeric members of this protein family, this protein exists as a homodimer and forms stable dimers with DNA elements. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (4) lacks an exon in the 5' UTR, compared to variant 1. Variants 1, 4 and 5 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Other Versions

Customer Reviews 
Loading...