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RAD17 (NM_133341) Human Untagged Clone
CAT＃: SC305944
RAD17 (untagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 4
CNY 9,500.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CCYC; HRAD17; R24L; RAD17SP; RAD24 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>SC305944 representing NM_133341.
Blue=Insert sequence Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGAATCAGCATGAACTTGCTGTGCATAAAAAGAAAATTGAAGAAGTCGAAACCTGGTTAAAAGCTCAA GTTTTAGAAAGGCAACCAAAACAGGGTGGATCTATTTTATTAATAACAGGTCCTCCTGGATGTGGAAAG ACAACGACCTTAAAAATACTATCAAAGGAGCATGGTATTCAAGTACAAGAGTGGATTAATCCAGTTTTA CCAGACTTCCAAAAAGATGATTTCAAGGGGATGTTTAATACTGAATCAAGCTTCCATATGTTTCCCTAT CAGTCTCAGATAGCAGTTTTCAAAGAGTTTCTACTAAGAGCGACAAAGTATAACAAGTTACAAATGCTT GGAGATGATCTGAGAACTGATAAGAAGATAATTCTGGTTGAAGATTTACCTAACCAGTTTTATCGGGAT TCTCATACTTTACATGAAGTTCTAAGGAAGTATGTGAGGATTGGTCGATGTCCTCTTATATTTATAATC TCGGACAGTCTCAGTGGAGATAATAATCAAAGGTTATTGTTTCCCAAAGAAATTCAGGAAGAGTGTTCT ATCTCAAATATTAGTTTCAACCCTGTGGCACCAACAATTATGATGAAATTTCTTAATCGAATAGTGACT ATAGAAGCTAACAAGAATGGAGGAAAAATTACTGTCCCTGACAAAACTTCTCTAGAGTTGCTCTGTCAG GGATGTTCTGGTGATATCAGAAGTGCAATAAACAGCCTCCAGTTTTCTTCTTCAAAAGGAGAAAACAAC TTACGGCCAAGGAAAAAAGGAATGTCTTTAAAATCAGATGCTGTGCTGTCAAAATCAAAACGAAGAAAA AAACCTGATAGGGTTTTTGAAAATCAAGAGGTCCAAGCTATTGGTGGCAAAGATGTTTCTCTGTTTCTC TTCAGAGCTTTGGGGAAAATTCTATATTGTAAAAGAGCATCTTTAACAGAATTAGACTCACCTCGGTTG CCCTCTCATTTATCAGAATATGAACGGGATACATTACTTGTTGAACCTGAGGAGGTAGTAGAAATGTCA CACATGCCTGGAGACTTATTTAATTTATATCTTCACCAAAACTACATAGATTTCTTCATGGAAATTGAT GATATTGTGAGAGCCAGTGAATTTCTGAGTTTTGCAGATATCCTCAGTGGTGACTGGAATACACGCTCT TTACTCAGGGAATATAGCACATCTATAGCTACGAGAGGTGTGATGCATTCCAACAAAGCCCGAGGATAT GCTCATTGCCAAGGAGGAGGATCAAGTTTTCGACCCTTGCACAAACCTCAGTGGTTTCTAATAAATAAA AAGTATCGGGAAAATTGCCTGGCAGCAAAAGCACTTTTTCCTGACTTCTGCCTACCAGCTTTATGCCTC CAAACTCAGCTATTGCCATACCTTGCTCTACTAACCATTCCAATGAGAAATCAAGCTCAGATTTCTTTT ATCCAAGATATTGGAAGGCTCCCTCTGAAGCGACACTTTGGAAGATTGAAAATGGAAGCCCTGACTGAC AGGGAACATGGAATGATAGACCCTGACAGCGGAGATGAAGCCCAGCTTAATGGAGGACATTCTGCAGAG GAATCTCTGGGTGAACCCACTCAAGCCACTGTGCCGGAAACCTGGTCTCTTCCTTTGAGTCAGAATAGT GCCAGTGAACTGCCTGCTAGCCAGCCCCAGCCCTTTTCAGCCCAAGGAGACATGGAAGAAAACATAATA ATAGAAGACTACGAGAGTGATGGGACATAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC |
| Restriction Sites | SgfI-MluI |
| ACCN | NM_133341 |
| Insert Size | 1755 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_133341.2 |
| RefSeq Size | 2593 bp |
| RefSeq ORF | 1755 bp |
| Locus ID | 5884 |
| UniProt ID | O75943 |
| Protein Families | Druggable Genome |
| MW | 66.2 kDa |
| Gene Summary | The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Multiple alternatively spliced transcript variants of this gene, which encode four distinct protein isoforms, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified. [provided by RefSeq, Jul 2013] Transcript Variant: This variant (4) lacks an internal exon in the 5' UTR and an in-frame exon in the 5' coding region, when compared to variant 1. The resulting isoform (4) is shorter and lacks an internal segment in the N-terminal region, as compared to isoform 1. |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219964 | RAD17 (Myc-DDK-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 4 |
CNY 4,352.00 |
|
| RC219964L3 | Lenti-ORF clone of RAD17 (Myc-DDK-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 4 |
CNY 7,030.00 |
|
| RC219964L4 | Lenti-ORF clone of RAD17 (mGFP-tagged)-Human RAD17 homolog (S. pombe) (RAD17), transcript variant 4 |
CNY 7,030.00 |
|
| RG219964 | RAD17 (tGFP-tagged) - Human RAD17 homolog (S. pombe) (RAD17), transcript variant 4 |
CNY 5,610.00 |
