CHD8 (NM_020920) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	AUTS18; HELSNF1
Vector	pCMV6-XL5
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for NM_020920, the custom clone sequence may differ by one or more nucleotides ATGAAGGGTGAATCGAAACGCATCACCCTGGTCCTCCAGCAGCCACAGTCTGGAGGTCCCCAAGGACATC GGCATGTTGTGCTAGGGAGTCTACCAGGCAAGATAGTGTTACAGGGCAACCAGCTGGCAGCCCTGACTCA AGCCAAGAATGCCCAAGGGCAGCCTGCCAAGGTAGTAACTATCCAGCTGCAGGTGCAGCAGCCACAGCAA AAAATCCAGATTGTACCACAACCACCATCATCGCAGCCACAGCCCCAGCAGCCACCCTCCACCCAGCCAG TGACTCTGTCCTCTGTACAGCAGGCTCAGATAATGGGACCAGGACAAAGCCCAGGACAAAGACTTTCAGT ACCAGTCAAGGTGGTACTGCAGCCACAGGCTGGCTCTTCCCAAGGGGCCTCTTCTGGGCTCTCTGTAGTT AAAGTTCTGAGTGCCAGTGAAGTGGCAGCTTTGTCATCACCAGCAAGCTCTGCTCCTCATTCGGGGGGAA AGACAGGAATGGAGGAAAACCGCAGATTGGAACACCAGAAGAAGCAAGAGAAAGCAAATCGGATTGTAGC AGAGGCCATTGCGAGAGCCCGTGCCCGCGGTGAGCAGAACATACCTCGAGTCTTAAATGAGGACGAGTTG CCCAGCGTTCGGCCAGAGGAGGAAGGCGAGAAGAAACGCAGGAAGAAGAGTGCTGGGGAGAGGCTGAAAG AGGAGAAGCCAAAGAAGAGTAAAACATCTGGTGCCTCCAAAACAAAGGGCAAGAGCAAGCTCAACACCAT CACTCCTGTAGTGGGTAAGAAGAGAAAACGTAATACCTCATCTGATAATTCAGATGTGGAAGTCATGCCT GCACAGTCACCTCGAGAAGATGAAGAAAGCAGCATTCAGAAGAGACGCTCAAACCGCCAAGTTAAGCGAA AAAAATATACAGAGGACCTGGATATAAAGATCACAGATGATGAAGAAGAAGAAGAGGTGGATGTAACTGG TCCAATAAAACCTGAGCCTATCCTCCCTGAACCAGTGCAAGAACCAGATGGCGAGACTTTGCCTTCCATG CAGTTCTTTGTGGAGAATCCCAGTGAAGAAGATGCAGCCATTGTAGACAAAGTGCTTTCTATGCGGATTG TGAAGAAGGAGCTCCCTTCTGGACAATATACTGAAGCAGAAGAATTCTTTGTCAAGTACAAGAACTACTC CTATCTGCATTGTGAATGGGCTACTATCTCCCAACTAGAGAAGGATAAGAGGATACATCAAAAATTAAAG CGCTTCAAAACCAAAATGGCTCAGATGAGACACTTCTTCCATGAGGATGAAGAGCCCTTTAATCCAGACT ACGTAGAGGTGGATAGGATATTGGATGAGTCTCACAGTATTGACAAGGACAATGGGGAGCCCGTTATTTA CTACCTGGTGAAATGGTGCTCTCTGCCCTATGAGGATAGCACATGGGAGCTAAAAGAAGATGTTGATGAG GGCAAGATTCGAGAATTTAAACGGATTCAGTCAAGGCACCCAGAACTCAAAAGGGTGAATCGTCCGCAGG CAAGTGCCTGGAAGAAATTGGAGCTATCACATGAATATAAAAACAGAAACCAGCTACGGGAATATCAGTT GGAAGGGGTTAATTGGCTGCTCTTTAATTGGTATAACAGGCAGAACTGCATCCTGGCTGATGAGATGGGA TTGGGCAAAACTATTCAGTCCATTGCCTTCTTGCAGGAAGTATATAATGTGGGCATCCATGGTCCCTTCT TGGTCATTGCCCCACTGTCCACAATTACTAACTGGGAGCGAGAATTTAATACATGGACAGAAATGAACAC TATTGTGTACCATGGCAGTCTGGCCAGCAGGCAGATGATTCAACAGTATGAAATGTACTGCAAAGATTCA CGGGGACGCCTCATCCCAGGCGCATACAAGTTTGACGCTCTGATCACCACTTTTGAGATGATTTTGTCAG ATTGTCCTGAGCTTCGTGAAATTGAATGGCGTTGTGTTATCATTGATGAAGCCCATCGACTGAAAAACCG TAATTGCAAGCTGCTTGATAGTCTCAAGCACATGGACCTGGAACACAAGGTGCTACTCACAGGAACACCA TTGCAAAATACTGTAGAAGAACTGTTTAGCTTGCTTCATTTCTTGGAACCGTCACAATTTCCCTCAGAAT CAGAGTTTCTCAAGGACTTTGGGGATCTCAAGACAGAGGAACAGGTTCAAAAGCTACAGGCCATTCTTAA GCCAATGATGCTGAGAAGACTCAAAGAGGATGTTGAAAAAAACTTGGCACCCAAACAGGAAACAATTATT GAAGTAGAGCTGACTAATATCCAGAAGAAATACTATCGGGCTATTTTGGAGAAGAATTTCTCCTTCCTTT CCAAAGGGGCAGGTCATACCAACATGCCTAATCTACTTAACACAATGATGGAGTTGCGCAAGTGCTGCAA CCACCCATATCTCATCAATGGTGCTGAAGAAAAAATCCTAACAGAATTCCGTGAAGCTTGCCATATTATA CCTCATGACTTTCACCTGCAGGCCATGGTTCGTTCAGCCGGCAAACTGGTTCTTATTGACAAGTTGCTTC CAAAGCTTAAAGCTGGTGGCCATAAAGTTCTGATCTTCTCTCAGATGGTGCGCTGCCTAGACATCCTAGA GGATTATTTAATCCAGAGGAGGTACTTATATGAACGTATTGATGGGCGAGTTAGAGGCAACCTTCGACAG GCTGCCATTGACCGCTTCAGCAAGCCTGACTCAGACCGCTTTGTCTTCTTACTGTGTACCCGGGCTGGTG GACTTGGTATTAATCTTACAGCTGCTGATACCTGCATCATCTTTGATTCAGACTGGAATCCACAAAATGA CCTGCAGGCCCAGGCACGATGTCATCGAATTGGGCAGAGCAAAGCTGTGAAGGTGTACCGCCTCATCACT CGTAATTCCTACGAGAGAGAGATGTTTGATAAGGCCAGCCTCAAGTTGGGGTTGGATAAGGCTGTGCTTC AATCCATGAGTGGTCGGGATGGCAACATTACTGGAATCCAACAGTTCTCTAAGAAGGAGATTGAAGATCT TTTAAGAAAAGGAGCATATGCAGCCATCATGGAGGAAGATGATGAAGGCTCCAAGTTTTGTGAAGAGGAC ATTGACCAGATCTTGTTAAGACGAACTACAACCATCACCATTGAATCTGAAGGAAAAGGTTCCACCTTTG CTAAGGCAAGCTTTGTTGCTTCTGAAAACAGGACAGATATTTCTTTGGATGACCCCAACTTTTGGCAAAA GTGGGCCAAAAAGGCTGACCTAGACATGGATCTGCTCAACAGCAAGAATAATTTGGTAATTGACACACCT AGAGTACGAAAACAAACGCGCCACTTTAGCACTCTGAAAGATGATGACCTGGTGGAATTCTCTGATTTGG AAAGTGAGGATGATGAGCGGCCACGCTCCCGCAGACATGACCGTCATCATGCCTATGGGCGCACTGACTG CTTTCGGGTGGAAAAGCATCTCCTGGTATATGGTTGGGGACGATGGCGAGATATTTTATCTCATGGACGC TTCAAGCGACGTATGACTGAACGAGATGTGGAGACCATTTGTCGGGCCATTCTCGTGTACTGTCTTCTAC ACTACCGTGGGGATGAAAATATTAAAGGCTTCATCTGGGACTTGATTAGCCCAGCTGAAAATGGCAAGAC AAAAGAATTGCAGAATCATTCAGGTCTATCTATCCCTGTGCCTCGTGGACGCAAAGGAAAAAAAGTAAAG TCACAAAGCACTTTTGATATCCATAAGGCAGATTGGATCCGGAAATATAACCCTGACACTTTGTTCCAAG ATGAAAGTTATAAGAAGCACTTGAAACATCAGTGTAACAAGGTACTGTTGCGGGTACGAATGCTATACTA CCTGAGGCAGGAGGTTATTGGAGACCAAGCAGAAAAGGTGTTAGGGGGTGCGATTGCCAGTGAGATTGAC ATATGGTTCCCAGTAGTGGATCAACTGGAGGTTCCAACAACTTGGTGGGACAGTGAGGCTGACAAGTCGC TGCTCATTGGAGTCTTTAAACATGGCTATGAGAAATATAATACCATGAGGGCAGACCCAGCCTTATGTTT CCTAGAAAAGGCTGGCCGACCAGATGACAAAGCAATTGCAGCAGAACATCGAGTGTTGGATAACTTCTCT GACATAGTAGAAGGGGTTGACTTTGATAAAGATTGTGAAGATCCTGAATATAAACCACTCCAAGGTCCCC CAAAGGACCAAGATGATGAGGGTGATCCCTTGATGATGATGGATGAGGAGATCTCAGTGATTGATGGAGA TGAAGCCCAGGTGACCCAACAGCCAGGCCATTTATTCTGGCCTCCGGGCTCTGCCCTAACAGCTAGGCTT CGGCGTCTAGTAACAGCGTATCAGCGCAGCTACAAGAGAGAACAAATGAAGATAGAGGCTGCAGAACGTG GGGACCGGCGAAGGCGGCGTTGTGAAGCAGCCTTCAAGCTGAAAGAAATTGCACGGCGGGAGAAACAACA ACGATGGACAAGGCGTGAACAAACTGATTTTTATCGAGTGGTGTCTACGTTTGGTGTGGAATATGACCCT GACACCATGCAGTTCCATTGGGATCGCTTCCGCACTTTTGCTCGACTAGACAAAAAGACAGATGAAAGCC TTACCAAGTACTTCCATGGCTTTGTGGCCATGTGCCGCCAAGTATGCCGCCTTCCCCCAGCAGCTGGAGA TGAACCCCCCGACCCTAACCTGTTCATTGAGCCCATCACTGAGGAGAGAGCCTCACGGACTCTCTACCGT ATAGAATTGCTTCGGCGCTTACGGGAACAAGTTTTATGCCACCCCCTTTTGGAAGATCGGCTGGCATTGT GTCAGCCTCCTGGTCCTGAATTGCCCAAATGGTGGGAGCCTGTTCGGCATGATGGGGAGCTTCTAAGAGG GGCAGCCCGCCATGGGGTGAGCCAAACAGACTGCAACATCATGCAGGACCCAGACTTCTCTTTTCTGGCT GCCCGTATGAATTATATGCAGAACCATCAAGCAGGAGCACCAGCTCCATCCTTGTCACGCTGCTCTACTC CACTGCTGCACCAGCAGTATACCTCACGCACTGCCTCACCACTGCCCCTGCGCCCAGATGCTCCTGTTGA AAAGTCACCCGAGGAGACAGCTACCCAGGTCCCCAGTCTGGAGAGTCTGACTTTAAAGCTAGAGCACGAG GTGGTGGCCAGGAGCCGACCAACCCCACAAGACTATGAGATGCGAGTATCCCCCTCTGATACTACCCCTC TGGTTTCCCGGAGTGTTCCACCAGTCAAACTGGAGGATGAGGATGATTCGGACTCTGAGCTGGACTTGAG CAAGCTGTCACCATCTTCTTCTTCTTCCTCATCCTCATCCAGCTCCAGCTCCAGCACTGATGAGAGTGAG GATGAGAAGGAAGAGAAGCTAACTGACCAGTCCCGCTCAAAGCTCTATGATGAAGAGAGTCTCCTGTCCC TCACTATGTCCCAAGATGGATTCCCAAATGAAGATGGAGAACAAATGACCCCTGAGCTTCTGCTACTGCA GGAAAGACAAAGAGCCTCTGAGTGGCCCAAGGATCGTGTCCTGATAAACCGTATTGACCTCGTCTGCCAG GCTGTACTCTCAGGGAAGTGGCCTTCTAGCCGTAGGAGCCAGGAAATGGTAACAGGAGGAATTTTGGGGC CAGGCAACCACTTGCTAGACAGTCCCTCATTGACTCCTGGAGAATATGGTGACTCTCCAGTCCCCACACC ACGAAGTAGTAGTGCAGCTTCCATGGCAGAGGAGGAAGCATCTGCAGTCAGCACAGCGGCAGCCCAGTTC ACCAAACTTCGCCGAGGCATGGATGAAAAGGAGTTTACAGTTCAAATCAAAGATGAGGAAGGATTGAAGT TAACATTCCAGAAGCACAAGTTGATGGCGAATGGAGTAATGGGAGATGGACATCCACTGTTTCATAAGAA GAAGGGGAACAGAAAGAAGCTAGTAGAGCTGGAGGTGGAGTGCATGGAAGAGCCTAATCACCTTGATGTG GACCTGGAGACCCGGATCCCTGTCATCAATAAGGTGGATGGTACTTTGCTGGTGGGTGAGGATGCCCCTC GCCGGGCTGAACTGGAGATGTGGTTACAGGGTCATCCAGAGTTTGCTGTTGATCCCCGATTTCTAGCGTA TATGGAGGATCGCAGAAAACAGAAGTGGCAAAGATGTAAAAAAAATAATAAGGCAGAATTGAACTGTTTG GGAATGGAACCAGTACAGACAGCTAACTCTAGAAATGGGAAAAAGGGTCATCACACTGAAACGGTGTTCA ACCGGGTTTTGCCAGGGCCTATTGCACCAGAGAGCAGCAAGAAGCGGGCCCGTAGGATGCGACCAGACCT TTCTAAGATGATGGCCCTCATGCAGGGTGGAAGCACTGGGTCTCTATCTCTGCATAACACGTTCCAACAC AGCAGTAGTGGCCTACAGTCTGTGTCATCTTTGGGTCACAGCAGTGCCACTTCTGCATCTTTGCCTTTTA TGCCATTTGTGATGGGTGGTGCACCATCATCCCCTCATGTAGACTCCAGCACCATGCTTCATCACCACCA CCACCACCCCCACCCCCACCATCACCACCATCACCATCCAGGCTTGAGAGCCCCTGGCTACCCCTCTTCA CCAGTGACTACCGCCTCTGGTACTACCTTGCGGTTGCCACCACTGCAACCTGAGGAGGATGACGATGAGG ATGAAGAAGATGATGATGACTTATCTCAGGGCTATGATAGCTCAGAAAGGGACTTCTCACTCATTGATGA TCCTATGATGCCAGCTAACTCAGACTCCAGTGAAGATGCTGATGACTGA
Restriction Sites	Please inquire
ACCN	NM_020920
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_020920.1, NP_065971.1
RefSeq Size	7499 bp
RefSeq ORF	6909 bp
Locus ID	57680
UniProt ID	Q9HCK8
Protein Pathways	Wnt signaling pathway
Gene Summary	This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which are common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. This gene has been shown to function in several processes that include transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Allelic variants of this gene are associated with autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016] Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and has a distinct 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.

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