CHD8 (NM_020920) Human Untagged Clone

CAT#: SC304865

CHD8 (untagged)-Human chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 2



  "NM_020920" in other vectors (2)

CNY 22,800.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (2)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms AUTS18; HELSNF1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_020920, the custom clone sequence may differ by one or more nucleotides


ATGAAGGGTGAATCGAAACGCATCACCCTGGTCCTCCAGCAGCCACAGTCTGGAGGTCCCCAAGGACATC
GGCATGTTGTGCTAGGGAGTCTACCAGGCAAGATAGTGTTACAGGGCAACCAGCTGGCAGCCCTGACTCA
AGCCAAGAATGCCCAAGGGCAGCCTGCCAAGGTAGTAACTATCCAGCTGCAGGTGCAGCAGCCACAGCAA
AAAATCCAGATTGTACCACAACCACCATCATCGCAGCCACAGCCCCAGCAGCCACCCTCCACCCAGCCAG
TGACTCTGTCCTCTGTACAGCAGGCTCAGATAATGGGACCAGGACAAAGCCCAGGACAAAGACTTTCAGT
ACCAGTCAAGGTGGTACTGCAGCCACAGGCTGGCTCTTCCCAAGGGGCCTCTTCTGGGCTCTCTGTAGTT
AAAGTTCTGAGTGCCAGTGAAGTGGCAGCTTTGTCATCACCAGCAAGCTCTGCTCCTCATTCGGGGGGAA
AGACAGGAATGGAGGAAAACCGCAGATTGGAACACCAGAAGAAGCAAGAGAAAGCAAATCGGATTGTAGC
AGAGGCCATTGCGAGAGCCCGTGCCCGCGGTGAGCAGAACATACCTCGAGTCTTAAATGAGGACGAGTTG
CCCAGCGTTCGGCCAGAGGAGGAAGGCGAGAAGAAACGCAGGAAGAAGAGTGCTGGGGAGAGGCTGAAAG
AGGAGAAGCCAAAGAAGAGTAAAACATCTGGTGCCTCCAAAACAAAGGGCAAGAGCAAGCTCAACACCAT
CACTCCTGTAGTGGGTAAGAAGAGAAAACGTAATACCTCATCTGATAATTCAGATGTGGAAGTCATGCCT
GCACAGTCACCTCGAGAAGATGAAGAAAGCAGCATTCAGAAGAGACGCTCAAACCGCCAAGTTAAGCGAA
AAAAATATACAGAGGACCTGGATATAAAGATCACAGATGATGAAGAAGAAGAAGAGGTGGATGTAACTGG
TCCAATAAAACCTGAGCCTATCCTCCCTGAACCAGTGCAAGAACCAGATGGCGAGACTTTGCCTTCCATG
CAGTTCTTTGTGGAGAATCCCAGTGAAGAAGATGCAGCCATTGTAGACAAAGTGCTTTCTATGCGGATTG
TGAAGAAGGAGCTCCCTTCTGGACAATATACTGAAGCAGAAGAATTCTTTGTCAAGTACAAGAACTACTC
CTATCTGCATTGTGAATGGGCTACTATCTCCCAACTAGAGAAGGATAAGAGGATACATCAAAAATTAAAG
CGCTTCAAAACCAAAATGGCTCAGATGAGACACTTCTTCCATGAGGATGAAGAGCCCTTTAATCCAGACT
ACGTAGAGGTGGATAGGATATTGGATGAGTCTCACAGTATTGACAAGGACAATGGGGAGCCCGTTATTTA
CTACCTGGTGAAATGGTGCTCTCTGCCCTATGAGGATAGCACATGGGAGCTAAAAGAAGATGTTGATGAG
GGCAAGATTCGAGAATTTAAACGGATTCAGTCAAGGCACCCAGAACTCAAAAGGGTGAATCGTCCGCAGG
CAAGTGCCTGGAAGAAATTGGAGCTATCACATGAATATAAAAACAGAAACCAGCTACGGGAATATCAGTT
GGAAGGGGTTAATTGGCTGCTCTTTAATTGGTATAACAGGCAGAACTGCATCCTGGCTGATGAGATGGGA
TTGGGCAAAACTATTCAGTCCATTGCCTTCTTGCAGGAAGTATATAATGTGGGCATCCATGGTCCCTTCT
TGGTCATTGCCCCACTGTCCACAATTACTAACTGGGAGCGAGAATTTAATACATGGACAGAAATGAACAC
TATTGTGTACCATGGCAGTCTGGCCAGCAGGCAGATGATTCAACAGTATGAAATGTACTGCAAAGATTCA
CGGGGACGCCTCATCCCAGGCGCATACAAGTTTGACGCTCTGATCACCACTTTTGAGATGATTTTGTCAG
ATTGTCCTGAGCTTCGTGAAATTGAATGGCGTTGTGTTATCATTGATGAAGCCCATCGACTGAAAAACCG
TAATTGCAAGCTGCTTGATAGTCTCAAGCACATGGACCTGGAACACAAGGTGCTACTCACAGGAACACCA
TTGCAAAATACTGTAGAAGAACTGTTTAGCTTGCTTCATTTCTTGGAACCGTCACAATTTCCCTCAGAAT
CAGAGTTTCTCAAGGACTTTGGGGATCTCAAGACAGAGGAACAGGTTCAAAAGCTACAGGCCATTCTTAA
GCCAATGATGCTGAGAAGACTCAAAGAGGATGTTGAAAAAAACTTGGCACCCAAACAGGAAACAATTATT
GAAGTAGAGCTGACTAATATCCAGAAGAAATACTATCGGGCTATTTTGGAGAAGAATTTCTCCTTCCTTT
CCAAAGGGGCAGGTCATACCAACATGCCTAATCTACTTAACACAATGATGGAGTTGCGCAAGTGCTGCAA
CCACCCATATCTCATCAATGGTGCTGAAGAAAAAATCCTAACAGAATTCCGTGAAGCTTGCCATATTATA
CCTCATGACTTTCACCTGCAGGCCATGGTTCGTTCAGCCGGCAAACTGGTTCTTATTGACAAGTTGCTTC
CAAAGCTTAAAGCTGGTGGCCATAAAGTTCTGATCTTCTCTCAGATGGTGCGCTGCCTAGACATCCTAGA
GGATTATTTAATCCAGAGGAGGTACTTATATGAACGTATTGATGGGCGAGTTAGAGGCAACCTTCGACAG
GCTGCCATTGACCGCTTCAGCAAGCCTGACTCAGACCGCTTTGTCTTCTTACTGTGTACCCGGGCTGGTG
GACTTGGTATTAATCTTACAGCTGCTGATACCTGCATCATCTTTGATTCAGACTGGAATCCACAAAATGA
CCTGCAGGCCCAGGCACGATGTCATCGAATTGGGCAGAGCAAAGCTGTGAAGGTGTACCGCCTCATCACT
CGTAATTCCTACGAGAGAGAGATGTTTGATAAGGCCAGCCTCAAGTTGGGGTTGGATAAGGCTGTGCTTC
AATCCATGAGTGGTCGGGATGGCAACATTACTGGAATCCAACAGTTCTCTAAGAAGGAGATTGAAGATCT
TTTAAGAAAAGGAGCATATGCAGCCATCATGGAGGAAGATGATGAAGGCTCCAAGTTTTGTGAAGAGGAC
ATTGACCAGATCTTGTTAAGACGAACTACAACCATCACCATTGAATCTGAAGGAAAAGGTTCCACCTTTG
CTAAGGCAAGCTTTGTTGCTTCTGAAAACAGGACAGATATTTCTTTGGATGACCCCAACTTTTGGCAAAA
GTGGGCCAAAAAGGCTGACCTAGACATGGATCTGCTCAACAGCAAGAATAATTTGGTAATTGACACACCT
AGAGTACGAAAACAAACGCGCCACTTTAGCACTCTGAAAGATGATGACCTGGTGGAATTCTCTGATTTGG
AAAGTGAGGATGATGAGCGGCCACGCTCCCGCAGACATGACCGTCATCATGCCTATGGGCGCACTGACTG
CTTTCGGGTGGAAAAGCATCTCCTGGTATATGGTTGGGGACGATGGCGAGATATTTTATCTCATGGACGC
TTCAAGCGACGTATGACTGAACGAGATGTGGAGACCATTTGTCGGGCCATTCTCGTGTACTGTCTTCTAC
ACTACCGTGGGGATGAAAATATTAAAGGCTTCATCTGGGACTTGATTAGCCCAGCTGAAAATGGCAAGAC
AAAAGAATTGCAGAATCATTCAGGTCTATCTATCCCTGTGCCTCGTGGACGCAAAGGAAAAAAAGTAAAG
TCACAAAGCACTTTTGATATCCATAAGGCAGATTGGATCCGGAAATATAACCCTGACACTTTGTTCCAAG
ATGAAAGTTATAAGAAGCACTTGAAACATCAGTGTAACAAGGTACTGTTGCGGGTACGAATGCTATACTA
CCTGAGGCAGGAGGTTATTGGAGACCAAGCAGAAAAGGTGTTAGGGGGTGCGATTGCCAGTGAGATTGAC
ATATGGTTCCCAGTAGTGGATCAACTGGAGGTTCCAACAACTTGGTGGGACAGTGAGGCTGACAAGTCGC
TGCTCATTGGAGTCTTTAAACATGGCTATGAGAAATATAATACCATGAGGGCAGACCCAGCCTTATGTTT
CCTAGAAAAGGCTGGCCGACCAGATGACAAAGCAATTGCAGCAGAACATCGAGTGTTGGATAACTTCTCT
GACATAGTAGAAGGGGTTGACTTTGATAAAGATTGTGAAGATCCTGAATATAAACCACTCCAAGGTCCCC
CAAAGGACCAAGATGATGAGGGTGATCCCTTGATGATGATGGATGAGGAGATCTCAGTGATTGATGGAGA
TGAAGCCCAGGTGACCCAACAGCCAGGCCATTTATTCTGGCCTCCGGGCTCTGCCCTAACAGCTAGGCTT
CGGCGTCTAGTAACAGCGTATCAGCGCAGCTACAAGAGAGAACAAATGAAGATAGAGGCTGCAGAACGTG
GGGACCGGCGAAGGCGGCGTTGTGAAGCAGCCTTCAAGCTGAAAGAAATTGCACGGCGGGAGAAACAACA
ACGATGGACAAGGCGTGAACAAACTGATTTTTATCGAGTGGTGTCTACGTTTGGTGTGGAATATGACCCT
GACACCATGCAGTTCCATTGGGATCGCTTCCGCACTTTTGCTCGACTAGACAAAAAGACAGATGAAAGCC
TTACCAAGTACTTCCATGGCTTTGTGGCCATGTGCCGCCAAGTATGCCGCCTTCCCCCAGCAGCTGGAGA
TGAACCCCCCGACCCTAACCTGTTCATTGAGCCCATCACTGAGGAGAGAGCCTCACGGACTCTCTACCGT
ATAGAATTGCTTCGGCGCTTACGGGAACAAGTTTTATGCCACCCCCTTTTGGAAGATCGGCTGGCATTGT
GTCAGCCTCCTGGTCCTGAATTGCCCAAATGGTGGGAGCCTGTTCGGCATGATGGGGAGCTTCTAAGAGG
GGCAGCCCGCCATGGGGTGAGCCAAACAGACTGCAACATCATGCAGGACCCAGACTTCTCTTTTCTGGCT
GCCCGTATGAATTATATGCAGAACCATCAAGCAGGAGCACCAGCTCCATCCTTGTCACGCTGCTCTACTC
CACTGCTGCACCAGCAGTATACCTCACGCACTGCCTCACCACTGCCCCTGCGCCCAGATGCTCCTGTTGA
AAAGTCACCCGAGGAGACAGCTACCCAGGTCCCCAGTCTGGAGAGTCTGACTTTAAAGCTAGAGCACGAG
GTGGTGGCCAGGAGCCGACCAACCCCACAAGACTATGAGATGCGAGTATCCCCCTCTGATACTACCCCTC
TGGTTTCCCGGAGTGTTCCACCAGTCAAACTGGAGGATGAGGATGATTCGGACTCTGAGCTGGACTTGAG
CAAGCTGTCACCATCTTCTTCTTCTTCCTCATCCTCATCCAGCTCCAGCTCCAGCACTGATGAGAGTGAG
GATGAGAAGGAAGAGAAGCTAACTGACCAGTCCCGCTCAAAGCTCTATGATGAAGAGAGTCTCCTGTCCC
TCACTATGTCCCAAGATGGATTCCCAAATGAAGATGGAGAACAAATGACCCCTGAGCTTCTGCTACTGCA
GGAAAGACAAAGAGCCTCTGAGTGGCCCAAGGATCGTGTCCTGATAAACCGTATTGACCTCGTCTGCCAG
GCTGTACTCTCAGGGAAGTGGCCTTCTAGCCGTAGGAGCCAGGAAATGGTAACAGGAGGAATTTTGGGGC
CAGGCAACCACTTGCTAGACAGTCCCTCATTGACTCCTGGAGAATATGGTGACTCTCCAGTCCCCACACC
ACGAAGTAGTAGTGCAGCTTCCATGGCAGAGGAGGAAGCATCTGCAGTCAGCACAGCGGCAGCCCAGTTC
ACCAAACTTCGCCGAGGCATGGATGAAAAGGAGTTTACAGTTCAAATCAAAGATGAGGAAGGATTGAAGT
TAACATTCCAGAAGCACAAGTTGATGGCGAATGGAGTAATGGGAGATGGACATCCACTGTTTCATAAGAA
GAAGGGGAACAGAAAGAAGCTAGTAGAGCTGGAGGTGGAGTGCATGGAAGAGCCTAATCACCTTGATGTG
GACCTGGAGACCCGGATCCCTGTCATCAATAAGGTGGATGGTACTTTGCTGGTGGGTGAGGATGCCCCTC
GCCGGGCTGAACTGGAGATGTGGTTACAGGGTCATCCAGAGTTTGCTGTTGATCCCCGATTTCTAGCGTA
TATGGAGGATCGCAGAAAACAGAAGTGGCAAAGATGTAAAAAAAATAATAAGGCAGAATTGAACTGTTTG
GGAATGGAACCAGTACAGACAGCTAACTCTAGAAATGGGAAAAAGGGTCATCACACTGAAACGGTGTTCA
ACCGGGTTTTGCCAGGGCCTATTGCACCAGAGAGCAGCAAGAAGCGGGCCCGTAGGATGCGACCAGACCT
TTCTAAGATGATGGCCCTCATGCAGGGTGGAAGCACTGGGTCTCTATCTCTGCATAACACGTTCCAACAC
AGCAGTAGTGGCCTACAGTCTGTGTCATCTTTGGGTCACAGCAGTGCCACTTCTGCATCTTTGCCTTTTA
TGCCATTTGTGATGGGTGGTGCACCATCATCCCCTCATGTAGACTCCAGCACCATGCTTCATCACCACCA
CCACCACCCCCACCCCCACCATCACCACCATCACCATCCAGGCTTGAGAGCCCCTGGCTACCCCTCTTCA
CCAGTGACTACCGCCTCTGGTACTACCTTGCGGTTGCCACCACTGCAACCTGAGGAGGATGACGATGAGG
ATGAAGAAGATGATGATGACTTATCTCAGGGCTATGATAGCTCAGAAAGGGACTTCTCACTCATTGATGA
TCCTATGATGCCAGCTAACTCAGACTCCAGTGAAGATGCTGATGACTGA


Restriction Sites Please inquire     
ACCN NM_020920
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_020920.1, NP_065971.1
RefSeq Size 7499 bp
RefSeq ORF 6909 bp
Locus ID 57680
UniProt ID Q9HCK8
Protein Pathways Wnt signaling pathway
Gene Summary This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which are common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. This gene has been shown to function in several processes that include transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Allelic variants of this gene are associated with autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016]
Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and has a distinct 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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