Retinol Saturase (RETSAT) (AK075261) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Vector	pCMV6-XL6
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >NCBI ORF sequence for AK075261, the custom clone sequence may differ by one or more nucleotides ATGTGGCTTCCGCTGGTGCTGCTCCTGGCTGTGCTGCTGCTGGCCGTCCTCTGCAAAGTTTACTTGGGAC TATTCTCTGGCAGCTCCCCGAATCCTTTCTCCGAAGATGTCAAACGGCCCCCAGCGCCCCTGGTAACTGA CAAGGAGGCCAGGAAGAAGGTTCTCAAACAAGCTTTTTCAGCCAACCAAGTGCCGGAGAAGCTGGATGTG GTGGTAATTGGCAGTGGCTTTGGGGGCCTGGCTGCAGCTGCAATTCTAGCTAAAGCTGGCAAGCGAGTCC TGGTGCTGGAACAACATACCAAGGCAGGGGGCTGCTGTCATACCTTTGGAAAGAATGGCCTTGAATTTGA CACAGGAATCCATTACATTGGGCGTATGGAAGAGGGCAGCATTGGCCGTTTTATCTTGGACCAGATCACT GAAGGGCAGCTGGACTGGGCTCCCCTGTCCTCTCCTTTTGACATCATGGTACTGGAAGGGCCCAATGGCC GAAAGGAGTACCCCATGTACAGTGGAGAGAAAGCCTACATTCAGGGCCTCAAGGAGAAGTTTCCACAGGA GGAAGCTATCATTGACAAGTATATAAAGCTGGTTAAGGTGGTATCCAGTGGAGCCCCTCATGCCATCCTG TTGAAATTCCTCCCATTGCCCGTGGTTCAGCTCCTCGACAGGTGTGGGCTGCTGACTCGTTTCTCTCCAT TCCTTCAAGCATCCACCCAGAGCCTGGCTGAGGTCCTGCAGCAGCTGGGGGCCTCCTCTGAGCTCCAGGC AGTACTCAGCTACATCTTCCCCACTTACGGTGTCACCCCCAACCACAGTGCCTTTTCCATGCACGCCCTG CTGGTCAACCACTACATGAAAGGAGGCTTTTATCCCCGAGGGGGTTCCAGTGAAATTGCCTTCCACACCA TCCCTGTGATTCAGCGGGCTGGGGGCGCTGTCCTCACAAAGGCCACTGTGCAGAGTGTGTTGCTGGACTC AGCTGGGAAAGCCTGTGGTGTCAGTGTGAAGAAGGGGCATGAGCTGGTGAACATCTATTGCCCCATCGTG GTCTCCAGCGCAGGACTGTTCAACACCTATGAACACCTACTGCCGGGGAACGCCCGCTGCCTGCCAGGTG TGAAGCAGCAACTGGGGACGGTGCGGCCCGGCTTAGGCATGACCTCTGTTTTCATCTGCCTGCGAGGCAC CAAGGAAGACCTGCATCTGCCGTCCACCAACTACTATGTTTACTATGACACGGACATGGACCAGGCGATG GAGCGCTACGTCTCCATGCCCAGGGAAGAGGCTGCGGAACACATCCCTCTTCTCTTCTTCGCTTTCCCAT CAGCCAAAGATCCGACCTGGGAGGACCGATTCCCAGGCCGGTCCACCATGATCATGCTCATACCCACTGC CTACGAGTGGTTTGAGGAGTGGCAGGCGGAGCTGAAGGGAAAGCGGGGCAGTGACTATGAGACCTTCAAA AACTCCTTTGTGGAAGCCTCTATGTCAGTGGTCCTGAAACTGTTCCCACAGCTGGAGGGGAAGGTGGAGA GTGTGACTGCAGGATCCCCACTCACCAACCAGTTCTATCTGGCTGCTCCCCGAGGTGCCTGCTACGGGGC TGACCATGACCTGGGCCGCCTGCACCCTTGTGTGATGGCCTCCTTGAGGGCCCAGAGCCCCATCCCCAAC CTCTATCTGACAGGCCAGGATATCTTCACCTGTGGACTGGTCGGGGCCCTGCAAGGTGCCCTGCTGTGCA GCAGCGCCATCCTGAAGCGGAACTTGTACTCAGACCTTAAGAATCTTGATTCTAGGATCCGGGCACAGAA GAAAAAGAATTAG 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK075261 unedited NTTCTTTCCCCCGCCCGTTGNCGCAAAGGGCGGTAGGCGTGTACGGTGGNGAGTCTATAT AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC CGCGAATTCGGCACGAGGGACGCCGGCGTGATGTGGCTTCCGCTGGTGCTGCTCCTGGCT GTGCTGCTGCTGGCCGTCCTCTGCAAAGTTTACTTGGGACTATTCTCTGGCAGCTCCCCG AATCCTTTCTCCGAAGATGTCAAACGGCCCCCAGCGCCCCTGGTAACTGACAAGGAGGCC AGGAAGAAGGTTCTCAAACAAGCTTTTTCAGCCAACCAAGTGCCGGAGAAGCTGGATGTG GTGGTAATTGGCAGTGGCTTTGGGGGCCTGGCTGCAGCTGCAATTCTAGCTAAAGCTGGC AAGCGAGTCCTGGTGCTGGAACAACATACCAAGGCAGGGGGCTGCTGTCATACCTTTGGA AAGAATGGCCTTGAATTTGACACAGGAATCCATTACATTGGGCGTATGGAAGAGGGCAGC ATTGGCCGTTTTATCTTGGACCAGATCACTGAAGGGCAGCTGGACTGGGCTCCCCTGTCC TCTCCTTTTGACATCATGGTACTGGAAGGGCCCAATGGCCGAAAGGAGTACCCCATGTAC AGTGGAGAGAAAGCCTACATTCAGGGCCTCAAGGAGAAGTTTCCACAGGAGGAAGCTATC ATTGACAAGTATATAAAGCTGGTTAAGGTGGTATCCAGTGGAGCCCCTCTGCCATCCTGT TGAAATTCCTCCATTGCCCGTGGTTCAGCTCCTCGACAGGTGTGGGCTGCTGACTCGTTT CTCTCCATTCCTTCAGCATNCACCCAAGCCTGNCTGANNGTCTGCAGCAGCTGNGGGCCC TCTCTGAGCTCCAGNCAGTACTCAGCTACATCTTCCCACTTNNACGTGTCACCCCCAACN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK075261 unedited CATCCATGGGACCTATTGGNGAATGGCAACTTCCCAGGNCCAGGNAAAGCACTGGGGNAG GGAGTCACAGGGCATGCCACCCGGGTTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAA TCTAGAGTCGAGTTTTTTTTTTTTTTTTTTAAAGCAATGGAATCAATAAATTTATTAATG TTACATTAACACGAACTACAAAGAGACCTTTCGTATGTCTGACACCAAAGACATAACTGA AAAGTCATTTTTCCAAACCTTGAGCTTGCATTCACCTACCTGTCTAACCCTCACATGTGC TAATTAACTGCAAATGCCATTTCTGGGCTTCACACACATTCCGTGGCTTTCCCTTTTCTG ATGTGACTTCCCTCCCTTACCCCACACCTCCCTGCACTGTCCCCTGCTGTGCCCTTGGCT GGAATGCCCTGCAGCCTGCTTCAGCCCAGCAAAGTATTCATCTTACCAGTCCATGCCCTG ACTCCTGATGTCACCCTTCCCTGCATCACCCTTCCCTGTGTATTTGGTGGATAAGGCTTG ATTGAGGCTCAGGTACTGAGTCCCTGCTGGCACATTGAGAACCAGCTGCCACCCCGATGA TAAGGAAGACAGACCCGGGACTTCCATATGAATTGGATATGATCATCTGACATGCACCCT ACTAACTGATGGATCCTGCTGTTACCCCAAAGCTCCAGACCACACTGGCCAGACACAGGC ATTTCCTGTCCCCTGAAGAGCCACTGAACCCAGCACAAAGAGATCTAGAGCTCTCCAAAC CTATGGAATGGCAACCAANACCATGCCCAGGTTTTGAGTTAAGCCTCATAGTCTCCCTTT TCCCTTGACGTGTAAAACTGGGCAGCGTTACCCTAAATGGATGAGAGCCNAACCTTCAGG AAAAAAC
Restriction Sites	NotI-NotI
ACCN	AK075261
Insert Size	3000 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK075261.1, BAC11505.1
RefSeq Size	3001 bp
RefSeq ORF	1833 bp
Locus ID	54884
Protein Families	Transmembrane
Protein Pathways	Retinol metabolism
Gene Summary	Catalyzes the saturation of all-trans-retinol to all-trans-13,14-dihydroretinol. Does not exhibit any activity toward all-trans-retinoic acid, nor 9-cis, 11-cis or 13-cis-retinol isomers. May play a role in the metabolism of vitamin A. Independently of retinol conversion, may regulate liver metabolism upstream of MLXIPL/ChREBP. May play a role in adipocyte differentiation.[UniProtKB/Swiss-Prot Function]

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