PI 3 Kinase Class 3 (PIK3C3) (AK022653) Human Untagged Clone

CAT#: SC127628

(untagged)-Human cDNA FLJ12591 fis, clone NT2RM4001313, moderately similar to PHOSPHATIDYLINOSITOL 3-KINASE VPS34-LIKE (EC 2.7.1.137)



CNY 5,064.00

CNY 6,270.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
Rabbit Polyclonal Anti-PIK3C3 Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms hVps34; VPS34; Vps34
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for AK022653, the custom clone sequence may differ by one or more nucleotides


TTATATTTAAGCATGGAGATGATTTACGTCAAGATCAACTTATTCTTCAAATCATTTCACTCATGGACAA
GCTGTTACGGAAAGAAAATCTGGACTTGAAATTGACACCTTATAAGGTGTTAGCCACCAGTACAAAACAT
GGCTTCATGCAGTTTATCCAGTCAGTTCCTGTGGCTGAAGTTCTTGATACAGAGGGAAGCATTCAGAACT
TTTTTAGAAAATATGCACCAAGTGAGAATGGGCCAAATGGGATTAGTGCTGAGGTCATGGACACTTACGT
TAAAAGCTGTGCTGGATATTGCGTGATCACCTATATACTTGGAGTTGGAGACAGGCACCTGGATAACCTT
TTGCTAACAAAAACAGGTAACAATTAATGACTACCAGTAGACATACATTGTATATGTCCATGGTTTTTAC
CCCTGAATCTATGTACTAACAAGATAAGTTGCGGCCTGGCGCGGTGGCTCACGCCCGTAATCCCAGCACT
TTGGGAGGCCAAGACGGGTGGATCACCTGAGGTTGGGAGTTCGAGACCAGCCTGACCAACATGGAGAAAC
CCCGTCTCTACTAAACACACAAAATTTGCCGGGCGTGGTGGTACATGCCTGTAATCCCAACTACTCAGGA
GGCTGAGGCAGGAGAATCGCTTGAACCCGGGAGGCGGAGGTTGCAGTGAGCCGAGATTGCACCATTGCAC
TGCAGACTAGGCAACAAGATCAAAACGCCATCTTAGAAAAAAAAAAAAAAAAAAGATGAGTTGCGTAATT
GAAGATAAATCAGAGAGTCTTAAAAATTCCTTTAAATGTTACCTTGGAAGAACATTTAGGGAAAGTCCTT
TCCCTTTTAAGAATTGCTTTAGTAGTAATCCTACAGATGGTCATTTTGCCCACACTTCTGTACTGGAAAA
TGCCTCTTTTTATGGAAAGCCAATAATGGATACAAGTAGCTTAGTCTCTTACTGTATAGCTCTAGTTAGA
TTGTTAAAATGTCCTTTCTCTTACAAAGTGTGCTTAATTCACTTTAATATCTACCTTTAATTATTTGCTT
TTCCCCTTGGAGTTCTATGGGATAATCATTCTCAGTCCCCTTTGTCCTGACAACTCTTTAGCCCTATGTA
TTTATCTTTATGCAGACAGCTTCCAGACTTACAGCTTTTTACCTCAGTCCATTTCATTAACTTCAAATTG
AAATATCGAACTGCTTACTTGAAATTGTCATTATATATGGCCAGTTGTAAAAGGTTGTCCTTTCCTCTAC
TACCTGCCTAAAACCTGTTTCTCACCTCATTTTTCCATTTCAATAGATAGCAACATCACCTACCCAGTTG
TTCAAGCTCTAGACCTCAAAGTCATTCTTAACATGTCTCTTGGCCTCATCTCATATACTGAACCTATTAG
CAAATCTTGTCTACTCCTCAACTCCAAAATAGATCATGAAGTTACCCACTGTCTTCATTTTTACTACTTT
GACCCCACTTGAAGCAAACAATATATGTTGCCTAAACAATTTGAATGAAACCTGAACCCTTTATTGGGGC
CTTGTGGCTTGTCCAGGCAGGCCATTTGTCTGAATGTCAAACCACTCCTGCCTCAGACAAGAGCCTTTGT
TGTTTTCTCTTTCTGGAAAGTTATTTATTCAGATCTTTGCAGGGCTGACTTTAGTTATTAAGTTGAAGTT
CTAATGGCATCTCTTTAGAGAAGCCCTTTCTAACTCCTTGATCTGTAATAACATATCTGCCCTCTTCCTC
CAATGCTCTCTACTTATTACTGTATTGTTTATAGCACTTATCACTAGCTGAAGTTACCTTTTTTGTTTAA
TTCAACAACTATTTATTGAACAGCTAGTAGATGCTGGGAATACCAAAGTTAACAGAAGAGACAGAAATCC
TTGCTCTCCTAGAGCTTGTTGAGTTCTTATCATCATCTTCTCCCCTTCAGGATGCAAGTTCCATGAGAGC
AGGAACCTTTGTCCATTGGGGTTACCCCCATGCCTCTGGACCTTGAACACCACCTAGCTAAAGTGTTCAA
TAAATCAGTGTTGAATTAATGATGAATAAATACTTCTGCTCTTC


>OriGene 3' read for AK022653 unedited
GTACCAAGCCGCAATCNANAGTCGAGTTTTTTTTTTTTTTTTTTTAAAAGGCGTTTTGAT
CTTGTTGCCTAGTCTGCAGTGCAATGGTGCAATCTCGGCTCACTGCAACCTCCGCCTCCC
GGGTTCAAGCGATTCTCCTGCCTCAGCCTCCTGAGTAGTTGGGATTACAGGCATGTACCA
CCACGCCCGGCAAATTTTGTGTGTTTATTTTATACGGGGTTTCTCCATGTTGGTCAGGCT
GGTCTCGAACTCCCAACCTCAGGTGATCCACCCGCCTTGGCCTCCCAAAGTGCTGGGATT
ACGGGCGTGAGCCACCGCGCCAGGCCGCAACTTATCTTGTTAGTACATAGATTCAGGGGT
AAAAACCATGGGCATATACAATGTATGTCTACTGGTAGTCATTAATTGTTACCTGTTTTT
GTTAGCAAAAGGTTATCCAGGTGCCTGTCTCCAACTCCAAGTATATAGGTGATCACGCAA
TATCCAGCACAGCTTTTAACGTAAGTGTCCATGACCTCAGCACTAATCCCATTTGGCCCA
TTCTCACTTGGTGCATATTTTCTAAAAAAGTTCTGAATGCTTCCCTCTGTATCAAGAACT
TCAGCCACAGGAACTGACTGGATAAACTGCATGAAGCCATGTTTTGTACTGGTGGCTAAC
ACCTTATAAGGTGTCAATTTCAAGTCCAGATTTTCTTTCCGTAACACCTTGTCCATGAGT
GAAATGATTTGAAGAATAAGTTGATCTTGACGTAAATCATCTCCATGCTTAAATATAACT
GGATATTTGCCTCCATCTTCCGTCTTANAAAACAACTGTGCAGGCATAAAGGCACTCTTA
AACAGTGTAGCTTGTTCCCGGAATATTCCTCTAATTNTCACTTGGGGTTTCTAAAGGCAA
CGGGATAAGTCCCACATCTGACAAATTATCTTT
Restriction Sites NotI-NotI     
ACCN AK022653
Insert Size 2840 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq AK022653.1
RefSeq Size 2074 bp
RefSeq ORF 2074 bp
Locus ID 5289
Domains PI3_PI4_kinase
Protein Families Druggable Genome
Protein Pathways Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system, Regulation of autophagy
Gene Summary Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed:20643123, PubMed:20208530). Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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