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GBE1 (NM_000158) Human Untagged Clone
CAT＃: SC120081
GBE1 (untagged)-Human glucan (1,4-alpha-), branching enzyme 1 (GBE1)
CNY 5,152.00
Product images
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | APBD; GBE; GSD4 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC120081 sequence for NM_000158 edited (data generated by NextGen Sequencing)
ATGGCGGCTCCGATGACTCCCGCGGCTCGGCCCGAGGACTACGAGGCGGCGCTCAATGCC GCCCTGGCTGACGTGCCCGAACTGGCCAGACTCCTGGAGATCGACCCGTACTTGAAGCCC TACGCCGTGGACTTCCAGCGCAGGTATAAGCAGTTTAGCCAAATTTTGAAGAACATTGGA GAAAATGAAGGTGGTATTGATAAGTTTTCCAGAGGCTATGAATCATTTGGCGTCCACAGA TGTGCTGATGGTGGTTTATACTGCAAAGAATGGGCCCCGGGAGCAGAAGGAGTTTTTCTT ACTGGAGATTTTAATGGTTGGAATCCATTTTCGTACCCATACAAAAAACTGGATTATGGA AAATGGGAGCTGTATATCCCACCAAAGCAGAATAAATCTGTACTCGTGCCTCATGGATCC AAATTAAAGGTAGTTATTACTAGTAAAAGCGGAGAGATCTTGTATCGTATTTCACCGTGG GCAAAGTATGTGGTTCGTGAAGGTGATAATGTGAATTATGATTGGATACACTGGGATCCA GAACACTCATATGAGTTTAAGCATTCCAGACCAAAGAAGCCACGGAGTCTAAGAATTTAT GAATCTCATGTGGGAATTTCTTCCCATGAAGGAAAAGTAGCTTCTTATAAACATTTTACA TGCAATGTACTACCAAGAATCAAAGGCCTTGGATACAACTGCATTCAGTTGATGGCAATC ATGGAGCATGCTTACTATGCCAGCTTTGGTTACCAAATCACAAGCTTCTTTGCAGCTTCC AGCCGTTATGGAACACCTGAAGAGCTACAAGAACTGGTAGACACAGCTCATTCCATGGGT ATCATAGTCCTCTTAGATGTGGTACACAGCCATGCTTCAAAAAATTCAGCAGATGGATTG AATATGTTTGATGGGACAGATTCCTGTTATTTTCATTCTGGACCTAGAGGGACTCATGAT CTTTGGGATAGCAGATTGTTTGCCTACTCCAGCTGGGAAGTTTTAAGATTCCTTCTGTCA AACATAAGATGGTGGTTGGAAGAATATCGCTTTGATGGATTTCGTTTTGATGGTGTTACG TCCATGCTTTATCATCACCATGGAGTGGGTCAAGGTTTCTCAGGTGATTACAGTGAATAT TTCGGACTACAAGTAGATGAAGATGCCTTGACTTACCTCATGTTGGCAAATCATTTGGTT CACACGCTGTGTCCCGATTCTATAACAATAGCTGAGGATGTATCAGGAATGCCAGCTCTG TGCTCTCCAATTTCCCAGGGAGGGGGTGGTTTTGACTATCGACTAGCCATGGCAATTCCA GATAAGTGGATTCAGCTACTTAAAGAGTTTAAAGATGAAGACTGGAACATGGGCGATATA GTATACACGCTCACAAACAGGCGCTACCTTGAAAAGTGCATTGCTTATGCAGAGAGCCAT GATCAGGCATTGGTTGGGGATAAGTCGCTGGCATTTTGGTTGATGGATGCCGAAATGTAT ACAAACATGAGTGTCCTGACTCCTTTTACTCCAGTTATTGATCGTGGAATACAGCTTCAT AAAATGATTCGACTCATTACGCATGGGCTTGGTGGAGAAGGCTATCTCAATTTCATGGGT AATGAATTTGGGCATCCTGAATGGTTAGACTTCCCAAGAAAAGGAAATAATGAGAGTTAC CATTATGCCAGGCGGCAGTTTCATTTAACTGACGACGACCTTCTTCGCTACAAGTTCCTA AATAATTTTGACAGGGATATGAATAGATTGGAAGAAAGATATGGTTGGCTTGCAGCTCCA CAGGCCTACGTGAGTGAAAAACATGAAGGCAATAAGATCATTGCTTTTGAAAGAGCAGGT CTTCTTTTCATTTTCAACTTCCATCCAAGCAAGAGCTACACTGACTACCGAGTTGGAACA GCATTGCCAGGGAAATTCAAAATTGTGCTAGATTCAGATGCAGCGGAATATGGAGGGCAT CAGAGACTGGACCACAGCACTGACTTTTTTTCTGAGGCTTTTGAACATAATGGGCGTCCC TATTCTCTTTTGGTGTACATTCCAAGCAGAGTGGCCCTCATCCTTCAGAATGTGGATCTG CCGAATTGA Clone variation with respect to NM_000158.3 >OriGene 5' read for NM_000158 unedited
GGATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCACGCCTAGGCGCCCTCC GGCTCCGCCCTAGCCGCCGCGTCCCAGCTAGAGCTCCAGCGCCCGCTCAGGCCCCACTCG ACCCTCTCGGGCCTCGGCTACTTGGACTGCGGCGGAATATGGCGGCTCCGATGACTCCCG CGGCTCGGCCCGAGGACTACGAGGCGGCGCTCAATGCCGCCCTGGCTGACGTGCCCGAAC TGGCCAGACTCCTGGAGATCGACCCGTACTTGAAGCCCTACGCCGTGGACTTCCAGCGCA GGTATAAGCAGTTTAGCCAAATTTTGAAGAACATTGGAGAAAATGAAGGTGGTATTGATA AGTTTTCCAGAGGCTATGAATCATTTGGCGTCCACAGATGTGCTGATGGTGGTTTATACT GCAAAGAATGGGCCCCGGGAGCAGAAGGAGTTTTTCTTACTGGAGATTTTAATGGTTGGA ATCCATTTTCGTACCCATACAAAAAACTGGATTATGGAAAATGGGAGCTGTATATCCCAC CAAAGCAGAATAAATCTGTACTCGTGCCTCATGGATCCAAATTAAAGGTAGTTATTACTA GTAAAAGCGGAGAGATCTTGTATCGTATTTCACCGTGGGCAAAGTATGTGGTTCGTGAAG GGTGATATGTGAATTATGATTGGATACACTGGGATCCAGAACACTCATATGAGNTTAAGC ATTCCAGACCAAAGAAGCCACGGAGTCTAAGAAATTATGAATCTCATGTGGGGAATTTCT TCCCATGGAAGGAAAGTAGCTTCCTTATAACCATTTTACATGCCATGGTACTANCCANGA ATCANAGGCCCTGGNATACCAACTGCATCAGNTGGATGGCANTCATGGGAGCATGCTTAC TATGCCAGNCTTTGGNTACCAAATCACAAGCTTCTTTGCAGCCTCCAGCCCGTTATGC >OriGene 3' read for NM_000158 unedited
CCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTACA AAAGCTTTTTATTGATTGAAATGAAAGACATTTTCTGAAATGCTACAATTACCATTTCAG GGCTTCAGAAAGACTGAGATACTGGCATTTAACAAGATACTTAGTGGACAAGAGATCACA AAATAAAACACAAAATGAGACACGGATGAGTTCATACGTAATGGCTTTTATATTACCTTG GATATAATTCTTTATATCACTACTGCATATTTATTACAGTATTTCTAAACACAATTTGAA AAAAAGAAACAAAGAAACAATCTTGGTACCTCATAATTTAGACCAAAAGATATCAGAACA AATAGAGCCACCAAATATCCATCTTAAATACTGGGTACATTTAACAGAAATAATCATACA TGACAAATGATTCAAATTTGAATTTAAAAGGATCAAATAATTAGCCAGGAAAGCAAAATG TTTTTAACATATTATATATAACACTTCCATTTGAGAATCCTAGCTGCTAAGATGACTTGA AAATATGGTCTAGGATTCCTAATATTTTAAACATATTCTCCTATCTACTTAAATAAAAGT TTGCTGTATTTGCATAAACCAATATTGAATTTCAGACACTTGATGGCTTGGCTAGACAAC TGTATTCTGAAAAGCATACATGTTATAAGCTGTGTGANCAGTGATACCAGAAAACAAAAC ACANATCTGCATCTGGTGGAGCTGAAATCAGGCCTCTTCAATTCGGCAGATCCACATTCT GAAGGATGAGGGCCACTCTGCTTGGNAGTACACCANAAGAGAATAGGNNACGCCATTATG TTCAAAAGCCTCAGAAAAAAATCAGTGCTGTGGTACAGTCTCTGATGCCCTNCATATCCG CTGCATCTGAATCTAGCACATTTTGAATTNCCTGGCA |
| Restriction Sites | NotI-NotI |
| ACCN | NM_000158 |
| Insert Size | 3160 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_000158.1, NP_000149.1 |
| RefSeq Size | 2913 bp |
| RefSeq ORF | 2109 bp |
| Locus ID | 2632 |
| UniProt ID | Q04446 |
| Domains | isoamylase_N, alpha-amylase, Aamy |
| Protein Families | Druggable Genome |
| Protein Pathways | Metabolic pathways, Starch and sucrose metabolism |
| Gene Summary | The protein encoded by this gene is a glycogen branching enzyme that catalyzes the transfer of alpha-1,4-linked glucosyl units from the outer end of a glycogen chain to an alpha-1,6 position on the same or a neighboring glycogen chain. Branching of the chains is essential to increase the solubility of the glycogen molecule and, consequently, in reducing the osmotic pressure within cells. Highest level of this enzyme are found in liver and muscle. Mutations in this gene are associated with glycogen storage disease IV (also known as Andersen's disease). [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC204152 | GBE1 (Myc-DDK-tagged)-Human glucan (1,4-alpha-), branching enzyme 1 (GBE1) |
CNY 5,144.00 |
|
| RC204152L3 | Lenti ORF clone of Human glucan (1,4-alpha-), branching enzyme 1 (GBE1), Myc-DDK-tagged |
CNY 7,700.00 |
|
| RC204152L4 | Lenti ORF clone of Human glucan (1,4-alpha-), branching enzyme 1 (GBE1), mGFP tagged |
CNY 7,700.00 |
|
| RG204152 | GBE1 (tGFP-tagged) - Human glucan (1,4-alpha-), branching enzyme 1 (GBE1) |
CNY 6,744.00 |
|
| SC320912 | GBE1 (untagged)-Human glucan (1,4-alpha-), branching enzyme 1 (GBE1) |
CNY 5,152.00 |
