GCNT2 (NM_001491) Human Untagged Clone

CAT#: SC119201

GCNT2 (untagged)-Human glucosaminyl (N-acetyl) transferase 2, I-branching enzyme (I blood group) (GCNT2), transcript variant 2



  "NM_001491" in other vectors (6)

CNY 3,656.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


GCNT2 rabbit polyclonal antibody
    • 25 ul

CNY 800.00
CNY 1,280.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms bA360O19.2; bA421M1.1; CCAT; CTRCT13; GCNT2C; GCNT5; IGNT; II; NACGT1; NAGCT1; ULG3
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC119201 sequence for NM_001491 edited (data generated by NextGen Sequencing)
ATGCCTTTATCAATGCGTTACCTCTTCATAATTTCTGTCTCTAGTGTAATTATTTTTATC
GTCTTCTCTGTGTTCAATTTTGGGGGAGATCCAAGCTTCCAAAGGCTAAATATCTCAGAC
CCTTTGAGGCTGACTCAAGTTTGCACATCTTTTATCAATGGAAAAACACGTTTCCTGTGG
AAAAACAAACTAATGATCCATGAGAAGTCTTCTTGCAAGGAATACTTGACCCAGAGCCAC
TACATCACAGCCCCTTTATCTAAGGAAGAAGCTGACTTTCCCTTGGCATATATAATGGTC
ATCCATCATCACTTTGACACCTTTGCAAGGCTCTTCAGGGCTATTTACATGCCCCAAAAT
ATCTACTGTGTTCATGTGGATGAAAAAGCAACAACTGAATTTAAAGATGCGGTAGAGCAA
CTATTAAGCTGCTTCCCAAACGCTTTTCTGGCTTCCAAGATGGAACCCGTTGTCTATGGA
GGGATCTCCAGGCTCCAGGCTGACCTGAACTGCATCAGAGATCTTTCTGCCTTCGAGGTC
TCATGGAAGTACGTTATCAACACCTGTGGGCAAGACTTCCCCCTGAAAACCAACAAGGAA
ATAGTTCAGTATCTGAAAGGATTTAAAGGTAAAAATATCACCCCAGGGGTGCTGCCCCCA
GCTCATGCAATTGGACGGACTAAATATGTCCACCAAGAGCACCTGGGCAAAGAGCTTTCC
TATGTGATAAGAACAACAGCGTTGAAACCGCCTCCCCCCCATAATCTCACAATTTACTTT
GGCTCTGCCTATGTGGCTCTATCAAGAGAGTTTGCCAACTTTGTTCTGCATGACCCACGG
GCTGTTGATTTGCTCCAGTGGTCCAAGGACACTTTCAGTCCTGATGAGCATTTCTGGGTG
ACACTCAATAGGATTCCAGGTGTTCCTGGCTCTATGCCAAATGCATCCTGGACTGGAAAC
CTCAGAGCTATAAAGTGGAGTGACATGGAAGACAGACACGGAGGCTGCCACGGCCACTAT
GTACATGGTATTTGTATCTATGGAAACGGAGACTTAAAGTGGCTGGTTAATTCACCAAGC
CTGTTTGCTAACAAGTTTGAGCTTAATACCTACCCCCTTACTGTGGAATGCCTAGAACTG
AGGCATCGCGAAAGAACCCTCAATCAGAGTGAAACTGCGATACAACCCAGCTGGTATTTT
TGA

Clone variation with respect to NM_001491.2
>OriGene 5' read for NM_001491 unedited
NGTTCAAATATTTGTATACGACTCACTATAGGGCGGCCGCGACATTCGCACGAGTCGGGA
TGAAACGGAATCGATTCCCAGCGTCTCCAACAGGCAGGAGTGAGTGGAGTATGTTGCAAA
ATAAGAACTCAGAGAAACGAGTGAGTTTGNGAAAAAAGACTTACAGATTTTGACGGTCTC
TTGACATTTCACCCTTCTTTGAGGCATGCCTTTATCAATGCGTTACCTCTTCATAATTTC
TGTCTCTAGTGTAATTATTTTTATCGTCTTCTCTGTGTTCAATTTTGGGGGAGATCCAAG
CTTCCAAAGGCTAAATATCTCAGACCCTTTGAGGCTGACTCAAGTTTGCACATCTTTTAT
CAATGGAAAAACACGTTTCCTGTGGAAAAACAAACTAATGATCCATGAGAAGTCTTCTTG
CAAGGAATACTTGACCCAGAGCCACTACATCACAGCCCCTTTATCTAAGGAAGAAGCTGA
CTTTCCCTTGGCATATATAATGGTCATCCATCATCACTTTGACACCTTTGCAAGGCTCTT
CAGGGCTATTTACATGCCCCAAAATATCTACTGTGTTCATGTGGATGAAAAAGCAACAAC
TGAATTTAAAGATGCGGTAGAGCAACTATTAAGCTGCTTCCCAAACGCTTTTCTGGCTTC
CAAGATGGAACCCGTTGTCTATGGNANGGATCTCCAGGCTCCAGGCTGACCTGAACTGCA
TCAGAGATCTTTCTGCCTTCGAAGTCTCATGGAAAGTACGTTATCAACACCTGTGGGCAA
GACTTNNCCCTGAAACCACAAGNAATAGTTCATATCTGAAAGATTTAAAGTAAAAATACC
CCAGGGGTGCTGCCCACTCAGCAATGGACGACTAAAATGTNCACAAGACACCTGGCAAGA
GCTTNCTAGTGATAGACAACACGTGAAAACGCTCCCCCATATCTAA
>OriGene 3' read for NM_001491 unedited
NNTTTGGTAAATCTAAGNAACCGCGGCCGCAATCTAGNGATCGATTTTTTTTTTTTTTTT
TTTTGGAAAACAATATATTAATTTATTCCCAAAAAAAAAAACATGGAACCTCATCGAAAA
CAAACATCAGATACAGTAGTTTCAGGGCATTCCCATGGGCTGTCAGAAACCCACTAGCCC
AAACACTATGCTCTTCCAACATTAAAGTATTTAAGACACAGGAGAGATGACCAACAACAG
ACATGTAAACATCAATTCCAGAAAGATTTCTTGTTCTACATGAGAAACATCCAGTGTAAA
ACGGTTCCTCTTCTCAGGTGCTGTCATTTAAATAAGTTGAAAAGAGACAATTTTTAAACT
CCACTGCTGACCCTGAGTGCATTCGCTATCCCCTCACCTATTTTGTTTTGGGACAAAGTC
TCGCTCTGTCACCCAGGCTGGAGTGCAGTGGGGCACTCTCAGCTCACTGTAACCTCCACC
TCCTGGGTTCAAGCGATTCTCATGCCTCAGCCTGCCAAATAGCTGGGATTACAGGCACAT
GCCACAAAGCCCGGCTAATTTTTATATTTTTTAGTAAAGATGGGGTTTCACCATGTCGGC
CAGCCTGGTCTGGAACTCCTGGCATCAAGTGATCTACCTGCCTTGGCCTCCCAAAGTGTT
GGGATTACAGGTGTGAGCCCACCACGCCCGGCCCAAGCCAGAGGTCTTGTGAGGGGGACT
AACCCATCCTGAGGGTCCTACCCTCGTGACCTCATCTAAACTTCCCTTACCAAAAGCCCC
CTCTTCAAAACCAATCACATTGGAGGGGTTAAGGCCCCGACCCTTGGAATTTGGGGGGGA
CCACAGCCCCTCTATAAGCCCAGGGGCGTTTGTGCTTTGGAGCCCCATCA
Restriction Sites NotI-NotI     
ACCN NM_001491
Insert Size 4700 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_001491.2, NP_001482.1
RefSeq Size 4691 bp
RefSeq ORF 1203 bp
Locus ID 2651
UniProt ID Q06430
Domains Branch
Protein Families Druggable Genome, Transmembrane
Protein Pathways Glycosphingolipid biosynthesis - lacto and neolacto series, Metabolic pathways
Gene Summary This gene encodes the enzyme responsible for formation of the blood group I antigen. The i and I antigens are distinguished by linear and branched poly-N-acetyllactosaminoglycans, respectively. The encoded protein is the I-branching enzyme, a beta-1,6-N-acetylglucosaminyltransferase responsible for the conversion of fetal i antigen to adult I antigen in erythrocytes during embryonic development. Mutations in this gene have been associated with adult i blood group phenotype. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Transcript Variant: This variant (2) represents the longest transcript and encodes isoform B. Sequence Note: This RefSeq record represents the GCNT2*001.1.1 allele.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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