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HCE (RNGTT) (NM_003800) Human Untagged Clone
CAT＃: SC117771
RNGTT (untagged)-Human RNA guanylyltransferase and 5'-phosphatase (RNGTT)
CNY 4,456.00
Product images
CNY 6,281.00
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | CAP1A; hCAP; HCE; HCE1 |
| Vector | pCMV6-XL5 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC117771 sequence for NM_003800 edited (data generated by NextGen Sequencing)
ATGGCTCACAACAAGATCCCGCCGCGGTGGCTGAACTGTCCCCGGCGCGGCCAGCCGGTG GCAGGAAGATTCTTACCTCTGAAGACAATGTTAGGACCAAGATATGATAGTCAAGTTGCT GAAGAAAATCGGTTCCATCCCAGCATGCTCTCAAATTACCTAAAGAGCCTAAAGGTTAAA ATGGGCTTGTTGGTGGACCTGACAAATACTTCAAGGTTCTATGACCGAAATGACATAGAA AAAGAAGGAATCAAATATATAAAACTTCAGTGTAAAGGACATGGTGAGTGCCCTACCACT GAGAATACTGAGACCTTTATTCGTCTGTGTGAGCGGTTTAATGAAAGAAATCCACCTGAA CTTATAGGTGTTCATTGTACTCATGGCTTCAATCGCACTGGTTTCCTCATATGTGCCTTT TTGGTGGAGAAAATGGATTGGAGTATCGAAGCAGCAGTTGCTACTTTTGCCCAAGCCAGA CCACCAGGAATCTACAAGGGTGATTATTTGAAGGAACTTTTTCGTCGGTATGGTGACATA GAGGAAGCACCACCCCCACCTCTATTGCCAGATTGGTGTTTTGAGGATGATGAAGACGAA GATGAGGATGAGGATGGAAAGAAGGAATCAGAACCCGGGTCAAGTGCTTCTTTTGGCAAA AGGAGAAAAGAACGGTTAAAACTGGGCGCTATTTTCTTGGAAGGTGTTACTGTTAAAGGT GTAACTCAAGTAACAACACAACCAAAGTTAGGAGAGGTACAGCAGAAGTGTCATCAATTC TGTGGCTGGGAAGGGTCTGGATTCCCTGGAGCACAGCCTGTTTCCATGGACAAGCAAAAT ATTAAACTTTTAGACCTGAAGCCATACAAAGTAAGCTGGAAAGCAGATGGTACTCGGTAC ATGATGTTGATTGATGGCACAAATGAAGTTTTTATGATTGATAGAGACAATTCAGTATTT CATGTTTCAAATCTGGAATTTCCATTTCGTAAAGATCTTCGTATGCATTTATCAAATACT CTCTTGGATGGCGAGATGATTATTGACAGAGTAAATGGACAGGCTGTTCCTAGATATTTG ATATATGACATAATTAAATTCAATTCACAGCCCGTTGGAGATTGTGATTTTAATGTTCGT CTGCAGTGTATAGAACGAGAAATTATAAGTCCTCGACACGAAAAAATGAAGACTGGGCTC ATTGACAAAACACAGGAACCATTTAGCGTCAGAAATAAGCCGTTTTTTGACATCTGTACT TCAAGAAAGCTACTTGAAGGAAATTTTGCCAAAGAAGTGAGCCATGAAATGGATGGACTT ATTTTTCAGCCTACTGGAAAATACAAACCTGGTCGATGTGATGATATTTTGAAATGGAAG CCTCCCAGTCTGAATTCTGTGGATTTTCGTCTAAAAATAACAAGAATGGGAGGAGAAGGG TTACTTCCTCAGAATGTTGGCCTCCTGTATGTTGGAGGTTATGAAAGACCCTTTGCACAA ATCAAGGTGACAAAAGAGCTGAAACAGTATGACAACAAAATTATAGAATGCAAATTTGAG AACAACAGCTGGGTCTTCATGAGACAGAGAACAGACAAAAGTTTTCCTAATGCCTACAAC ACTGCCATGGCTGTGTGTAACAGCATCTCAAACCCTGTCACCAAGGAGATGCTGTTTGAG TTCATCGACAGATGTACTGCAGCTTCTCAAGGACAGAAGCGAAAACATCATCTGGACCCT GACACGGAGCTCATGCCACCACCACCTCCCAAAAGACCACGCCCTTTAACCTAA Clone variation with respect to NM_003800.3 >OriGene 5' read for NM_003800 unedited
GATTAGGGNCGGCACGCGCAATTCGGCACGAGGCTGTGGAGTTGATCCTGAATGAAAGTG GCGCGCCGCCCCTGACGTTACCCGGATCGGAGAGGTTGGAATTCATATTACGGCTGCGAT TCGGGTGTCTCGGACCCCGGTGTGCACCGGACCACGGGGAGGCGGCTCCAAAGGCGCGGT GAACGTTGGTGATGGATGGCAGCTCTGCGCAGCCCCAAGACATGGCTCACAACAAGATCC CGCCGCGGTGGCTGAACTGTCCCCGGCGCGGCCAGCCGGTGGCAGGAAGATTCTTACCTC TGAAGACAATGTTAGGACCAAGATATGATAGTCAAGTTGCTGAAGAAAATCGGTTCCATC CCAGCATGCTCTCAAATTACCTAAAGAGCCTAAAGGTTAAAATGGGCTTGTTGGTGGACC TGACAAATACTTCAAGGTTCTATGACCGAAATGACATAGAAAAAGAAGGAATCAAATATA TAAAACTTCAGTGTAAAGGACATGGTGAGTGCCCTACCACTGAGAATACTGAGACCTTTA TTCGTCTGTGTGAGCGGTTTAATGAAAGATATCCACCTGAACTTATAGGTGTTCATTGTA CTCATGGCTTCAATCGCACTGGTTTCCTCATATGTGCCTTTTTGGTGGAGAAAATGGATT GGAGTATCNGATCAGCAGTTGCTACTTTTGCCCAGCCAGACCACCAGGAATCTACAAGGG TGATTATTTTGAAGAACTTTTTCGTCNGTTTGGTGACATAGAGGAAGCACCACCCCCACC TCTATTGCCAAATATGGTGTTTTGAGGATGATGAANACCATNATGANGATGAGCGATGGA AAGATAGAATCAAAACCCGGGTCAAGTGCTTTCTTTTGGGCAAAGGNAGAAAGAATCGGT TAAAACTGGCA >OriGene 3' read for NM_003800 unedited
NTTGTGACTAGNACGCGGCCGCTTTTNANGATCGNGTTTTTTTTTTTTTTTTTTAATTTA AATCAGTAGTTTTATTACAATACGCACTGACACACAATTGGNAAAAGGAATGTCCTGACA TTTTCTGAGCATTTCACGGAAAGCAAATGTAACCATCCACGCAGTAGAGTCATCCACACC TTTTCCTAACAAAAAGCAAAAGCTTTCACATGTGATAACTGAAGAAAGCAAAGTTTTCTT ATTCTTGTCTAAAAAACTTCAGTCTCAGAAAATAGTTATTGAAGTGAGCTGAGAAGGAGC ACATTTCCTTCACAATGTCCAAAATCCTGCCTCTAGATTATCCCCCTGACCTTCTAAATG ACTTTTATAATACTGCAGATGGTATTATGTTCAGGGGCTAAATGGGATCTACTTATAAGG CCAACTACTCTATGTCACTATAATTGAAAGGAAAGAATAAATTAAGAAAATTAAGTCCTG CTAAAATGCACAAAGACTTGAAGAAAATATCTGGGTGTATTAAAAGTGAGATGCTCTTTT CACGTAACTCATTTGAAAGGTTACAATTACCATAAACATTNTAACTCCATTAAAGATCAT TGGCAAAAGCTTGCTTTCGTATCTGCAAAGGGCTCTGCCTTTCCACAGGCTCGGAGGGAA AGCAGCACACCGTGGAAGAGTCACTCGTCCTGGGGAAAGCAGCATCAGACTATNTGAAGT TGCATAGGCATTNTTTTTTTAATCCATTTNTGTAAAGATACTATGGGACCTTGAGAATTT TCCCTCCTTCATAGTCATTGGGCATCAGTTGTTTTATCCCTTTACGCCCAAGTGCAAAAA CCTCTAGTAAAAAACTTACAATGGGCGTTTGATAAATCACTATACATTACTGACTTGGGA A |
| Restriction Sites | NotI-NotI |
| ACCN | NM_003800 |
| Insert Size | 4110 bp |
| OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_003800.1, NP_003791.1 |
| RefSeq Size | 4546 bp |
| RefSeq ORF | 1794 bp |
| Locus ID | 8732 |
| UniProt ID | O60942 |
| Domains | mRNA_cap_C |
| Protein Families | Druggable Genome, Phosphatase |
| Gene Summary | Bifunctional mRNA-capping enzyme exhibiting RNA 5'-triphosphatase activity in the N-terminal part and mRNA guanylyltransferase activity in the C-terminal part. Catalyzes the first two steps of cap formation: by removing the gamma-phosphate from the 5'-triphosphate end of nascent mRNA to yield a diphosphate end, and by transferring the gmp moiety of GTP to the 5'-diphosphate terminus.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). |
Documents
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| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC202229 | RNGTT (Myc-DDK-tagged)-Human RNA guanylyltransferase and 5'-phosphatase (RNGTT) |
CNY 4,448.00 |
|
| RC202229L1 | Lenti ORF clone of Human RNA guanylyltransferase and 5'-phosphatase (RNGTT), Myc-DDK-tagged |
CNY 6,848.00 |
|
| RC202229L2 | Lenti ORF clone of Human RNA guanylyltransferase and 5'-phosphatase (RNGTT), mGFP tagged |
CNY 5,890.00 |
|
| RC202229L3 | Lenti ORF clone of Human RNA guanylyltransferase and 5'-phosphatase (RNGTT), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC202229L4 | Lenti ORF clone of Human RNA guanylyltransferase and 5'-phosphatase (RNGTT), mGFP tagged |
CNY 5,890.00 |
|
| RG202229 | RNGTT (tGFP-tagged) - Human RNA guanylyltransferase and 5'-phosphatase (RNGTT) |
CNY 6,048.00 |
