Rab5 (RAB5A) (NM_004162) Human Untagged Clone

CAT#: SC117557

RAB5A (untagged)-Human RAB5A, member RAS oncogene family (RAB5A)



  "NM_004162" in other vectors (7)

CNY 3,600.00


货期*
现货

规格
    • 10 ug

Cited in 4 publications.

Product images

经常一起买 (3)
RAB5A mouse monoclonal antibody,clone OTI6D9
    • 100 ul

CNY 1,999.00
CNY 2,700.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms RAB5
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC117557 sequence for NM_004162 edited (data generated by NextGen Sequencing)
ATGGCTAGTCGAGGCGCAACAAGACCCAACGGGCCAAATACTGGAAATAAAATATGCCAG
TTCAAACTAGTACTTCTGGGAGAGTCCGCTGTTGGCAAATCAAGCCTAGTGCTTCGTTTT
GTGAAAGGCCAATTTCATGAATTTCAAGAGAGTACCATTGGGGCTGCTTTTCTAACCCAA
ACTGTATGTCTTGATGACACTACAGTAAAGTTTGAAATATGGGATACAGCTGGTCAAGAA
CGATACCATAGCCTAGCACCAATGTACTACAGAGGAGCACAAGCAGCCATAGTTGTATAT
GATATCACAAATGAGGAGTCCTTTGCAAGAGCAAAAAATTGGGTTAAAGAACTTCAGAGG
CAAGCAAGTCCTAACATTGTAATAGCTTTATCGGGAAACAAGGCCGACCTAGCAAATAAA
AGAGCAGTAGATTTCCAGGAAGCACAGTCCTATGCAGATGACAATAGTTTATTATTCATG
GAGACATCCGCTAAAACATCAATGAATGTAAATGAAATATTCATGGCAATAGCTAAAAAA
TTGCCAAAGAATGAACCACAAAATCCAGGAGCAAATTCTGCCAGAGGAAGAGGAGTAGAC
CTTACCGAACCCACACAACCAACCAGGAATCAGTGTTGTAGTAACTAA

Clone variation with respect to NM_004162.4
42 g=>t
>OriGene 5' read for NM_004162 unedited
TTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGCTAAGAGCAGGCGA
CGCCGCCGCCGCCACCACCACCGCCATAGATACACTCTCATCCTACGGGCCACGCCTGGG
CCTTGCTGCCAGGAAGCTTCGGCCCCGCAGCTCGGCTTGCTGCGGTCTCAGGTTTCTTTA
CCTCCAGAAAGAAGAATATTGGCCCCTTGAATTCTGGAAGTTCATTGAAGAGTCTGAAAT
TAGGGACTTATTTCAAATTTGGACATGGCTAGTCGAGGCGCAACAAGACCCAACGGGCCA
AATACTGGAAATAAAATATGCCAGTTCAAACTAGTACTTCTGGGAGAGTCCGCTGTTGGC
AAATCAAGCCTAGTGCTTCGTTTTGTGAAAGGCCAATTTCATGAATTTCAAGAGAGTACC
ATTGGGGCTGCTTTTCTAACCCAAACTGTATGTCTTGATGACACTACAGTAAAGTTTGAA
ATATGGGATACAGCTGGTCAAGAACGATACCATAGCCTAGCACCAATGTACTACAGAGGA
GCACAAGCAGCCATAGTTGTATATGATATCACAAATGAGGAGTCCTTTGCAAGAGCAAAA
AATTGGGTTAAAGAACTTCAGAGGCAAGCAAGTCCTAACATTGTAATAGCTTTATCGGGA
AACAAGGCCGACCTAGCAATAAAAGAGCAGTAGATTTCCAGGAAGCACAGTCCTATGCAG
ATGACAATAGTTTATTATTCATGGAGACATCCGCTAAACATCAATGAATGTAAATGAAAT
ATTCATGGGCATAGCNTANNAATTGCCAAAAGATGACCACANATCCAGNAGCANATTCTG
CCAGAGGAGNAGGAGTAGACCTTACCGACCCCACNANCANCAGNNNATCATGGTGTAGTA
ACTAAC
>OriGene 3' read for NM_004162 unedited
GCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCTTTTTATACCACTTTATTCC
AACCTGAGCACCTCAATATAAAACTAAACACTGGTGAACCGTTTTCCTAATTCTGCATTA
TCATAAATGTACAAGTTCTCCTAGCAGTCCAACACTTAAATAGATTAAATCATCTCTGAC
ACATGGTAGCTTTCATATAATGAAAATACCTAAAATAATTAGTGCAATATACTGAACTGA
TCAAAATAAAATGAACTTTGGAAAGGAAGGCTGCAAGATTGTTACTAACATATTCCAATA
CTTTATGTTACAAATTACGGGTACATTGTTTATTATGGTTCTAGCCATGGAGGAACAACT
GAAGCCCCTTCCTGTCAAAGGGGGAGAGAGAAATAATTAGCTGTTTTAGTAACAGTACAT
TTTGTATGCTTTTAAGCAACTCTTAAATATTACAGAAAAGTAATACAGATATGGAGACTA
CAATGCAGTACCCTATTTACAGTACAGCTGAAGATCGAATTTAAAATAATCAAGTTTGAA
TATACATAATTGTTAGTGCATTGACTATATTATGTCAAAAGGGAACAAAAGCCCCCTCCC
CACCCCAAACAACAAAACCTGTGTAATGGCCAGCGGTGATTAATTAGAAATCACAGACAC
CTGGTTTATTTCATTTTTGTTAAAGAACTCACATGTTTGAAAGGAACACTGACCATTAAA
GTGCATTNATTAGTTCTCAAAATTCCTAAAACAAGATGGGGTTGCATCTGATTTTTACCC
CATTTAAAAAAAAAAACCAGACTGGGTATAATAAGATACCATTATACTCTTCCCACCCTG
ACCTGTTATGTGGGATATTTTCTTGGGGTAATTATTAAATGTGGAGACATTTTATACAGT
ACCAGAGAATGACATTGCTGNACTATGCTAGCATATAATACTTAAATCTCTCTAACAGAA
GT
Restriction Sites NotI-NotI     
ACCN NM_004162
Insert Size 2190 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004162.3, NP_004153.2
RefSeq Size 2352 bp
RefSeq ORF 648 bp
Locus ID 5868
UniProt ID P20339
Domains ras, RAN, RAS, RHO, RAB
Protein Families Druggable Genome
Protein Pathways Amyotrophic lateral sclerosis (ALS), Endocytosis
Gene Summary The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB5A is required for the fusion of plasma membranes and early endosomes (PubMed:10818110, PubMed:14617813, PubMed:16410077, PubMed:15378032). Contributes to the regulation of filopodia extension (PubMed:14978216). Required for the exosomal release of SDCBP, CD63, PDCD6IP and syndecan (PubMed:22660413). Regulates maturation of apoptotic cell-containing phagosomes, probably downstream of DYN2 and PIK3C3 (By similarity).[UniProtKB/Swiss-Prot Function]
Transcript Variant: This variant (1) represents the longer transcript and encodes the longer protein (isoform 1).
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Citations (4)

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