ATP6J (ATP6V1G1) (NM_004888) Human Untagged Clone
CAT#: SC117079
ATP6V1G1 (untagged)-Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 (ATP6V1G1)
CNY 1,200.00
CNY 2,950.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | ATP6G; ATP6G1; ATP6GL; ATP6J; Vma10 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>NCBI ORF sequence for NM_004888, the custom clone sequence may differ by one or more nucleotides
ATGGCTAGTCAGTCTCAGGGGATTCAGCAGCTGCTGCAGGCCGAGAAGCGGGCAGCCGAGAAGGTGTCCG AGGCCCGCAAAAGAAAGAACCGGAGGCTGAAGCAGGCCAAAGAAGAAGCTCAGGCTGAAATTGAACAGTA CCGCCTGCAGAGGGAGAAAGAATTCAAGGCCAAGGAAGCTGCGGCATTGGGATCCCGTGGCAGTTGCAGC ACTGAAGTGGAGAAGGAGACCCAGGAGAAGATGACCATCCTCCAGACATACTTCCGGCAGAACAGGGATG AAGTCTTGGACAACCTCTTGGCTTTTGTCTGTGACATTCGGCCAGAAATCCATGAAAACTACCGCATAAA TGGATAG >OriGene 5' read for NM_004888 unedited
GCACGAGGGCCGCTTGCCTTGCTCTCAGAATCGCTGCCGCCATGGCTAGTCAGTCTCAGG GGATTCAGCAGCTGCTGCAGGCCGAGAAGCGGGCAGCCGAGAAGGTGTCCGAGGCCCGCA AAAGAAAGAACCGGAGGCTGAAGCAGGCCAAAGAAGAAGCTCAGGCTGAAATTGAACAGT ACCGCCTGCAGAGGGAGAAAGAATTCAAGGCCAAGGAAGCTGCGGCATTGGGATCCCGTG GCAGTTGCAGCACTGAAGTGGAGAAGGAGACCCAGGAGAAGATGACCATCCTCCAGACAT ACTTCCGGCAGAACAGGGATGAAGTCTTGGACAACCTCTTGGCTTTTGTCTGTGACATTC GGCCAGAAATCCATGAAAACTACCGCATAAATGGATAGAAGAGAGAAGCACCTGTGCTGT GGAGTGGCATTTTAGATGCCCTCACGAATATGAAGCTTAGCACAGCTCTAGTTACATTCT TATGATATGGCATTAAATTATTTCCATATATTATATAATAGGTCCTTCCACTTTTTGGAG AGTAGCAAATCTAGCTTTTTTGTACAGACTTAGAAATTATCTAAAGATTTCATCT >OriGene 3' read for NM_004888 unedited
GCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTAAAAATGCATGACAGAGTC TTTACTTTTAAATGATTATCGATACACCAAGTAATACATGTAACAAGTTCTTGAATTCTA TCATCTAGTAATTTTGATTAAGAGAAACTAAAAGCAGCCCAAACAATTCCACTAGTATTC ACTGTTCTAACCATTAGCAAGAATGGACTACTTTAAGGCTGGCTGCTGCTTCACACAGGT TACAAAGAACTATTTACTACTTTTTCATAGATAAAGCCCCTGACCTTCAAGAAAGTGTTA GGGAAAAAAATTATTTAATCCCTTCCTTTCTTCAAAGAATTGTTATGTGGTTTTTTTTTT TTTAAACTAGATCTAAGAAAGAAAAAGTCAACACTGATATACATGTTGCTTGAGCCAAAA GACATAGGAAAAAAAGACAACATATAACCATTAAATTCCTAAGAAATATGAGGTAAAAAG ATGAAATCTTTAGATAATTTCTAAGTCTGTACAAAAAAGCTAGATTTGCTACTCTCCAAA AAGTGGAAGGACCTATTATATAATATATGGAAATAATTTAATGCCATATCATAAGAATGT AACTAGAGCTGTGCTAAGCTTCATATTCGTGAGGGCATCTAAAATGCCACTCCACAGCAC AGGTGCTTCTCTCTTCTATCCATTTATGCGGTAGTTTTCATGGATTTCTGGCCGAATGTC ACAGACAAAAGCCAAGAGGTTTGCCCAAGACTTTATCCCTGTTCTGCCCGAAGTTTGTCT GGAGGATGGGCACTCTCTCCTGGGTCTCCTCTCCACTTAGTGCTGAACTGCACGGGATCC CATGCCGCAGCTCCCTTGGCCTGAATCTTTCTCCCTTGAGGCGGGCTGTTAATTTAACTC GGCTTTTCTTTGGCTGCTTAACCCCGGTTTTTTTTGGGAGGCTAGAAACTT |
Restriction Sites | NotI-NotI |
ACCN | NM_004888 |
Insert Size | 980 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_004888.2, NP_004879.1 |
RefSeq Size | 1110 bp |
RefSeq ORF | 357 bp |
Locus ID | 9550 |
UniProt ID | O75348 |
Domains | V-ATPase_G |
Protein Pathways | Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection |
Gene Summary | This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of three V1 domain G subunit proteins. Pseudogenes of this gene have been characterized. [provided by RefSeq, Jul 2008] |
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203317 | ATP6V1G1 (Myc-DDK-tagged)-Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 (ATP6V1G1) |
CNY 1,200.00 |
|
RC203317L1 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 (ATP6V1G1), Myc-DDK-tagged |
CNY 3,600.00 |
|
RC203317L2 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 (ATP6V1G1), mGFP tagged |
CNY 5,890.00 |
|
RC203317L3 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 (ATP6V1G1), Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203317L4 | Lenti ORF clone of Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 (ATP6V1G1), mGFP tagged |
CNY 5,890.00 |
|
RG203317 | ATP6V1G1 (tGFP-tagged) - Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 (ATP6V1G1) |
CNY 2,800.00 |