ATP6J (ATP6V1G1) (NM_004888) Human Untagged Clone

CAT#: SC117079

ATP6V1G1 (untagged)-Human ATPase, H+ transporting, lysosomal 13kDa, V1 subunit G1 (ATP6V1G1)



  "NM_004888" in other vectors (6)

CNY 1,200.00

CNY 2,950.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
ATP6V1G1 Antibody - middle region
    • 100 ul

CNY 5,250.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms ATP6G; ATP6G1; ATP6GL; ATP6J; Vma10
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>NCBI ORF sequence for NM_004888, the custom clone sequence may differ by one or more nucleotides


ATGGCTAGTCAGTCTCAGGGGATTCAGCAGCTGCTGCAGGCCGAGAAGCGGGCAGCCGAGAAGGTGTCCG
AGGCCCGCAAAAGAAAGAACCGGAGGCTGAAGCAGGCCAAAGAAGAAGCTCAGGCTGAAATTGAACAGTA
CCGCCTGCAGAGGGAGAAAGAATTCAAGGCCAAGGAAGCTGCGGCATTGGGATCCCGTGGCAGTTGCAGC
ACTGAAGTGGAGAAGGAGACCCAGGAGAAGATGACCATCCTCCAGACATACTTCCGGCAGAACAGGGATG
AAGTCTTGGACAACCTCTTGGCTTTTGTCTGTGACATTCGGCCAGAAATCCATGAAAACTACCGCATAAA
TGGATAG


>OriGene 5' read for NM_004888 unedited
GCACGAGGGCCGCTTGCCTTGCTCTCAGAATCGCTGCCGCCATGGCTAGTCAGTCTCAGG
GGATTCAGCAGCTGCTGCAGGCCGAGAAGCGGGCAGCCGAGAAGGTGTCCGAGGCCCGCA
AAAGAAAGAACCGGAGGCTGAAGCAGGCCAAAGAAGAAGCTCAGGCTGAAATTGAACAGT
ACCGCCTGCAGAGGGAGAAAGAATTCAAGGCCAAGGAAGCTGCGGCATTGGGATCCCGTG
GCAGTTGCAGCACTGAAGTGGAGAAGGAGACCCAGGAGAAGATGACCATCCTCCAGACAT
ACTTCCGGCAGAACAGGGATGAAGTCTTGGACAACCTCTTGGCTTTTGTCTGTGACATTC
GGCCAGAAATCCATGAAAACTACCGCATAAATGGATAGAAGAGAGAAGCACCTGTGCTGT
GGAGTGGCATTTTAGATGCCCTCACGAATATGAAGCTTAGCACAGCTCTAGTTACATTCT
TATGATATGGCATTAAATTATTTCCATATATTATATAATAGGTCCTTCCACTTTTTGGAG
AGTAGCAAATCTAGCTTTTTTGTACAGACTTAGAAATTATCTAAAGATTTCATCT
>OriGene 3' read for NM_004888 unedited
GCGGCACGCAATCTAGAATCGAGTTTTTTTTTTTTTTTTTTAAAAATGCATGACAGAGTC
TTTACTTTTAAATGATTATCGATACACCAAGTAATACATGTAACAAGTTCTTGAATTCTA
TCATCTAGTAATTTTGATTAAGAGAAACTAAAAGCAGCCCAAACAATTCCACTAGTATTC
ACTGTTCTAACCATTAGCAAGAATGGACTACTTTAAGGCTGGCTGCTGCTTCACACAGGT
TACAAAGAACTATTTACTACTTTTTCATAGATAAAGCCCCTGACCTTCAAGAAAGTGTTA
GGGAAAAAAATTATTTAATCCCTTCCTTTCTTCAAAGAATTGTTATGTGGTTTTTTTTTT
TTTAAACTAGATCTAAGAAAGAAAAAGTCAACACTGATATACATGTTGCTTGAGCCAAAA
GACATAGGAAAAAAAGACAACATATAACCATTAAATTCCTAAGAAATATGAGGTAAAAAG
ATGAAATCTTTAGATAATTTCTAAGTCTGTACAAAAAAGCTAGATTTGCTACTCTCCAAA
AAGTGGAAGGACCTATTATATAATATATGGAAATAATTTAATGCCATATCATAAGAATGT
AACTAGAGCTGTGCTAAGCTTCATATTCGTGAGGGCATCTAAAATGCCACTCCACAGCAC
AGGTGCTTCTCTCTTCTATCCATTTATGCGGTAGTTTTCATGGATTTCTGGCCGAATGTC
ACAGACAAAAGCCAAGAGGTTTGCCCAAGACTTTATCCCTGTTCTGCCCGAAGTTTGTCT
GGAGGATGGGCACTCTCTCCTGGGTCTCCTCTCCACTTAGTGCTGAACTGCACGGGATCC
CATGCCGCAGCTCCCTTGGCCTGAATCTTTCTCCCTTGAGGCGGGCTGTTAATTTAACTC
GGCTTTTCTTTGGCTGCTTAACCCCGGTTTTTTTTGGGAGGCTAGAAACTT
Restriction Sites NotI-NotI     
ACCN NM_004888
Insert Size 980 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_004888.2, NP_004879.1
RefSeq Size 1110 bp
RefSeq ORF 357 bp
Locus ID 9550
UniProt ID O75348
Domains V-ATPase_G
Protein Pathways Epithelial cell signaling in Helicobacter pylori infection, Metabolic pathways, Oxidative phosphorylation, Vibrio cholerae infection
Gene Summary This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A, three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. The protein encoded by this gene is one of three V1 domain G subunit proteins. Pseudogenes of this gene have been characterized. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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