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ERO1L (ERO1A) (NM_014584) Human Untagged Clone
CAT＃: SC114943
ERO1L (untagged)-Human ERO1-like (S. cerevisiae) (ERO1L)
CNY 5,488.00
Product images
Specifications
| Product Data | |
| Type | Human Untagged Clone |
| Tag | Tag Free |
| Synonyms | ERO1-alpha; ERO1-L; ERO1-L-alpha; Ero1alpha; ERO1L; ERO1LA |
| Vector | pCMV6-XL4 |
| E. coli Selection | Ampicillin (100 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
>OriGene ORF within SC114943 sequence for NM_014584 edited (data generated by NextGen Sequencing)
ATGGGCCGCGGCTGGGGATTCTTGTTTGGCCTCCTGGGCGCCGTGTGGCTGCTCAGCTCG GGCCACGGAGAGGAGCAGCCCCCGGAGACAGCGGCACAGAGGTGCTTCTGCCAGGTTAGT GGTTACTTGGATGATTGTACCTGTGATGTTGAAACCATTGATAGATTTAATAACTACAGG CTTTTCCCAAGACTACAAAAACTTCTTGAAAGTGACTACTTTAGGTATTACAAGGTAAAC CTGAAGAGGCCGTGTCCTTTCTGGAATGACATCAGCCAGTGTGGAAGAAGGGACTGTGCT GTCAAACCATGTCAATCTGATGAAGTTCCTGATGGAATTAAATCTGCGAGCTACAAGTAT TCTGAAGAAGCCAATAATCTCATTGAAGAATGTGAACAAGCTGAACGACTTGGAGCAGTG GATGAATCTCTGAGTGAGGAAACACAGAAGGCTGTTCTTCAGTGGACCAAGCATGATGAT TCTTCAGATAACTTCTGTGAAGCTGATGACATTCAGTCCCCTGAAGCTGAATATGTAGAT TTGCTTCTTAATCCTGAGCGCTACACTGGTTACAAGGGACCAGATGCTTGGAAAATATGG AATGTCATCTACGAAGAAAACTGTTTTAAGCCACAGACAATTAAAAGACCTTTAAATCCT TTGGCTTCTGGTCAAGGGACAAGTGAAGAGAACACTTTTTACAGTTGGCTAGAAGGTCTC TGTGTAGAAAAAAGAGCATTCTACAGACTTATATCTGGCCTACATGCAAGCATTAATGTG CATTTGAGTGCAAGATATCTTTTACAAGAGACCTGGTTAGAAAAGAAATGGGGACACAAC ATTACAGAATTTCAACAGCGATTTGATGGAATTTTGACTGAAGGAGAAGGTCCAAGAAGG CTTAAGAACTTGTATTTTCTCTACTTAATAGAACTAAGGGCTTTATCCAAAGTGTTACCA TTCTTCGAGCGCCCAGATTTTCAACTCTTTACTGGAAATAAAATTCAGGATGAGGAAAAC AAAATGTTACTTCTGGAAATACTTCATGAAATCAAGTCATTTCCTTTGCATTTTGATGAG AATTCATTTTTTGCTGGGGATAAAAAAGAAGCACACAAACTAAAGGAGGACTTTCGACTG CATTTTAGAAATATTTCAAGAATTATGGATTGTGTTGGTTGTTTTAAATGTCGTCTGTGG GGAAAGCTTCAGACTCAGGGTTTGGGCACTGCTCTGAAGATCTTATTTTCTGAGAAATTG ATAGCAAATATGCCAGAAAGTGGACCTAGTTATGAATTCCATCTAACCAGACAAGAAATA GTATCATTATTCAACGCATTTGGAAGAATTTCTACAAGTGTGAAAGAATTAGAAAACTTC AGGAACTTGTTACAGAATATTCATTAA Clone variation with respect to NM_014584.1 >OriGene 5' read for NM_014584 unedited
ATGTTCGNAATTTGTNATACGACTCACTATAGGCGGCCGCGNAATTCGCACCAGGTTGTG CGCGTCGGGTTGCGTCTCCAGGCCGGGGGCGTCAGGGAGCCGGGGGCGGGGACGGGCGCG GGCGCAGCGGACCGGGCGGGGCCGAGCCGGGAAGCTGGTCGGTGCGTCAGCCTCACGCGG CGGGAAGGAACCGGTCCGAGGCCCCGGGCTGCCGGCGCGGGCGCCGCGGCACGTCCACAG GCTGGGTCGCGAGGTGGCGATCGCTGAGAGGCAGGAGGGCCGAGGCGGGCCTGGGAGGCG GCCCGGAGGTGGGGCGCCGCTGGGGCCGGCCCGCACGGGCTTCATCTGAGGGCGCACGGC CCGCGACCGAGCGTGCGGACTGGCCTCCCAAGCGTGGGGCGACAAGCTGCCGGAGCTGCA ATGGGCCGCGGCTGGGGATTCTTGTTTGGCCTCCTGGGCGCCGTGTGGCTGCTCAGCTCG GGCCACGGAGAGGAGCAGCCCCCGGAGACAGCGGCACAGAGGTGCTTCTGCCAGGTTAGT GGTTACTTGGATGATTGTACCTGTGATGTTGAAACCATTGATAGATTTAATAACTACAGG CTTTTCCCAAGACTACAAAAACTTCTTGAAAGTGACTACTTTAGGTATTACAAGGTAAAC CTGAAGAGGCCGTGTCCTTTCTGGAATGACATCAGCCAGTGTGGAAGAAGGGACTGTGCT GTCAAACCATGTCAATCTGATGAAGTTCCTGATGGAATTAAATCTGCGAGCTACAAGTAT TCTGAAGAAAGCCATAATCTCATTTGAAGAATGTGAACAAGCTGAACGACTTGNAGCAGT GGATGAATCTCTGAGTGAGGAACACAGAAGGCTGTTCTTTAGTGGACCAGGCAGATGATC CTTAGATACCTTCTGGGAAGCTGAGAAATTCAG >OriGene 3' read for NM_014584 unedited
NAAAATTTCACTTTGAACCCGCGGCCGCATNCTANGATCGAGTTTTTTTTTTTTTTTTTT TTTTAAAAATGTGTTTACTTAAAACAATATAATTCTCCTTTACAAAAGCAACTTTATATA AAATGTTTGGCTTAAGACTGTCATTGCTATTATGCCTTTGAATGAAATTCCACTCTTTCG CCTCCATTGTCCAGAAACAGGCACATATCAGCTTGTTTTCTTTAATGAATATTCTGTAAC AAGTTCCTGAAGTTTTCTAATTCTTTCACACTTGTAGAAATTCTTCCAAATGCGTTGAAT AATGATACTATTTCTTGTCTGGTTAGATGGAATTCATAACTAGGTCCACTTTCTGGCATA TTTGCTATCAATTTCTCAGAAAATAAGATCTTCAGAGCAGTGCCCAAACCCTGAGTCTGA AGCTTTCCCCACAGACGACATTTAAAACAACCAACACAATCCATAATTCTTGAAATATTT CTAAAATGCAGTCGAAAGTCCTCCTTTAGTTTGTGTGCTTCTTTTTTATCCCCAGCAAAA AATGAATTCTCATCAAAATGCAAAGGAAATGACTTGATTTCATGAAGTATTTCCAGAAGT AACATTTTGTTTTCCTCATCCTGAATTTTATTTCCAGTAAAGAGTTGAAAATCTGGGCGC TCGAAGAATGGTAACACTTTGGATAAAGCCCTTAGTTCTATTAAGTAGAGAAAATACAAG TTCTTAAGCCTTCTTGGACCTTCTCCTTCAGTCAAAATTCCATCAAATCGCTGTTGAAAT TCTGTAATGTTGGTGTCCCCATTTCTTTTCTAACCAGGTCTCTTGTAAAAGATATCTCGC ACTCCAATGCACATTAATGCTTGCATGTAGGCCAAATTAAGTCTGTAGATGCTCTTTTTT CTACCAG |
| Restriction Sites | NotI-NotI |
| ACCN | NM_014584 |
| Insert Size | 1910 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_014584.1, NP_055399.1 |
| RefSeq Size | 3334 bp |
| RefSeq ORF | 1407 bp |
| Locus ID | 30001 |
| UniProt ID | Q96HE7 |
| Domains | ERO1 |
| Protein Pathways | Vibrio cholerae infection |
| Gene Summary | Oxidoreductase involved in disulfide bond formation in the endoplasmic reticulum. Efficiently reoxidizes P4HB/PDI, the enzyme catalyzing protein disulfide formation, in order to allow P4HB to sustain additional rounds of disulfide formation. Following P4HB reoxidation, passes its electrons to molecular oxygen via FAD, leading to the production of reactive oxygen species (ROS) in the cell. Required for the proper folding of immunoglobulins. Involved in the release of the unfolded cholera toxin from reduced P4HB/PDI in case of infection by V.cholerae, thereby playing a role in retrotranslocation of the toxin. Plays an important role in ER stress-induced, CHOP-dependent apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1.[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203840 | ERO1L (Myc-DDK-tagged)-Human ERO1-like (S. cerevisiae) (ERO1L) |
CNY 5,488.00 |
|
| RC203840L1 | Lenti ORF clone of Human ERO1-like (S. cerevisiae) (ERO1L), Myc-DDK-tagged |
CNY 7,888.00 |
|
| RC203840L2 | Lenti ORF clone of Human ERO1-like (S. cerevisiae) (ERO1L), mGFP tagged |
CNY 5,890.00 |
|
| RC203840L3 | Lenti ORF clone of Human ERO1-like (S. cerevisiae) (ERO1L), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203840L4 | Lenti ORF clone of Human ERO1-like (S. cerevisiae) (ERO1L), mGFP tagged |
CNY 7,888.00 |
|
| RG203840 | ERO1L (tGFP-tagged) - Human ERO1-like (S. cerevisiae) (ERO1L) |
CNY 7,088.00 |
|
| SC322267 | ERO1L (untagged)-Human ERO1-like (S. cerevisiae) (ERO1L) |
CNY 5,488.00 |
