Ubiquitin (UBB) (NM_018955) Human Untagged Clone

CAT#: SC113349

UBB (untagged)-Human ubiquitin B (UBB)



  "NM_018955" in other vectors (7)

CNY 3,600.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (3)
Anti-UBB Rabbit Polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00


TurboFectin Transfection Reagent (1 mL in 1 vial)
    • 1 ml in 1 vial

CNY 4,090.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Synonyms HEL-S-50
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC113349 sequence for NM_018955 edited (data generated by NextGen Sequencing)
ATGCAGATCTTCGTGAAAACCCTTACCGGCAAGACCATCACCCTTGAGGTGGAGCCCAGT
GACACCATCGAAAATGTGAAGGCCAAGATCCAGGATAAGGAAGGCATTCCCCCCGACCAG
CAGAGGCTCATCTTTGCAGGCAAGCAGCTGGAAGATGGCCGTACTCTTTCTGACTACAAC
ATCCAGAAGGAGTCGACCCTGCACCTGGTCCTGCGTCTGAGAGGTGGTATGCAGATCTTC
GTGAAGACCCTGACCGGCAAGACCATCACCCTGGAAGTGGAGCCCAGTGACACCATCGAA
AATGTGAAGGCCAAGATCCAGGATAAAGAAGGCATCCCTCCCGACCAGCAGAGGCTCATC
TTTGCAGGCAAGCAGCTGGAAGATGGCCGCACTCTTTCTGACTACAACATCCAGAAGGAG
TCGACCCTGCACCTGGTCCTGCGTCTGAGAGGTGGTATGCAGATCTTCGTGAAGACCCTG
ACCGGCAAGACCATCACTCTGGAGGTGGAGCCCAGTGACACCATCGAAAATGTGAAGGCC
AAGATCCAAGATAAAGAAGGCATCCCCCCCGACCAGCAGAGGCTCATCTTTGCAGGCAAG
CAGCTGGAAGATGGCCGCACTCTTTCTGACTACAACATCCAGAAAGAGTCGACCCTGCAC
CTGGTCCTGCGCCTGAGGGGTGGCTGTTAA

Clone variation with respect to NM_018955.2
>OriGene 5' read for NM_018955 unedited
TGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGGTCCCTGTGGGTGGAC
GTGGTTGGTGATTGGCAGGATCCTGGTATCCGCTAACAGGTCAAAATGCAGATCTTCGTG
AAAACCCTTACCGGCAAGACCATCACCCTTGAGGTGGAGCCCAGTGACACCATCGAAAAT
GTGAAGGCCAAGATCCAGGATAAGGAAGGCATTCCCCCCGACCAGCAGAGGCTCATCTTT
GCAGGCAAGCAGCTGGAAGATGGCCGTACTCTTTCTGACTACAACATCCAGAAGGAGTCG
ACCCTGCACCTGGTCCTGCGTCTGAGAGGTGGTATGCAGATCTTCGTGAAGACCCTGACC
GGCAAGACCATCACCCTGGAAGTGGAGCCCAGTGACACCATCGAAAATGTGAAGGCCAAG
ATCCAGGATAAAGAAGGCATCCCTCCCGACCAGCAGAGGCTCATCTTTGCAGGCAAGCAG
CTGGAAGATGGCCGCACTCTTTCTGACTACAACATCCAGAAGGAGTCGACCCTGCACCTG
GTCCTGCGTCTGAGAGGTGGTATGCAGATCTTCGTGAAGACCCTGACCGGCAAGACCATC
ACTCTGGAGGTGGAGCCCAGTGACACCATCGAAAATGTGAAGGCCAAGATCCAAGATAAA
GAAGGCATCCCCCCCGACCAGCAGAGGCTCATCTTTGCAGGCAAGCAGCTGGAAGATGGC
CGCACTCTTTCTGACTACAACATCCAGAAAGAGTCGACCCTGCACCTGGTCCTGCGCCTG
AGGGGTGGCTGNTAATTCTTCAGTCATGGCATTCGCAGTGCCCAGTGATGGCATTACTCT
GCACTATAGCCATTTGCCCCAACTAAAAGTTAGAATACAGNTNCAGTATAGCTGAACCTG
TCAAATGTATAAAGGTTCGTGCATGTAGCAAAAAAAA
>OriGene 3' read for NM_018955 unedited
NAATACTGTGNACCGCGGCCGCATTCTANNGATCGGTTTTTTCTTTTTTTTTTTGCTACC
ATGCAACGAAACCTTTATTAACATTTTGAACAGGTTCAGCTATTACTGAAACTTGTAATT
TCTAAACTTAAGTTGGGGCAAATGGCTATAGTGCAGAGTAATGCCATCACTGGGCACTGC
GAATGCCATGACTGAAGAATTAACAGCCACCCCTTATGCGCAGGACCAGGTGCAGGGTCG
ACTCTTTCTGGATGTTGTAGTCAGAAAGAGTGCGGCCATCTTCCAGCTGCTTGCCTGCAA
AGATGAGCCTCTGCTGGTCGGGGGGGATGCCTTCTTTATCTTGGATCTTGGCCTTCACAT
TTTCGATGGTGTCACTGGGCTCCACCTCCAGAGTGATGGTCTTGCCGGTCAGGGTCTTCA
CGAAGATCTGCATACCACCTCTCAGACGCAGGACCAGGTGCAGGGTCGACTCCTTCTGGA
TGTTGTAGTCAGAAAGAGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATGAGCCTCT
GCTGGTCGGGAGGGATGCCTTCTTTATCCTGGATCTTGGCCTTCACATTTTCGATGGTGT
CACTGGGCTCCACTTCCAGGGTGATGGTCTTGCCGGTCAGGGTCTTCACGAAGATCTGCA
TACCACCTCTCAGACGCANGACCAGGTGCAGGGTCGACTCCTTCTGGATGTTGTAGTCAG
AAAAGTACGGCCATCTTCCAGCTGCTTGCCTGCAAAGATGAGCCTCTGCTGGTCGGGGGG
GATGCCTTNCCTATCCTGGATCTGGNCCTTCACATTTTCGAGGTGTCACTGGGCTTCAAC
TCCAAGGTGATGGTCTTGCCGTTAGGGTTTTCACGAAAATCTGCATTTGACCTGTTACCG
AAACCAGGATACTGGCAT
Restriction Sites NotI-NotI     
ACCN NM_018955
Insert Size 1000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_018955.2, NP_061828.1
RefSeq Size 971 bp
RefSeq ORF 690 bp
Locus ID 7314
UniProt ID P0CG47
Domains UBQ
Protein Families Druggable Genome
Protein Pathways Parkinson's disease
Gene Summary This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013]
Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, 3, 4, 5, and 6 encode the same protein.
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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