SMURF1 (NM_181349) Human Untagged Clone
CAT#: SC112098
SMURF1 (untagged)-Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2
CNY 5,360.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene sequence for NM_181349 edited
GAATTCGGCACGAGGCGCTGGATCCGCGGCTGCCCGATCGTTGGCGGGAGATGTCGAACC CCGGGACACGCAGGAACGGCTCCAGCATCAAGATCCGTCTGACAGTGTTATGTGCCAAGA ACCTTGCAAAGAAAGACTTCTTCAGGCTCCCTGACCCTTTTGCAAAGATTGTCGTGGATG GGTCTGGGCAGTGCCACTCAACCGACACTGTGAAAAACACATTGGACCCAAAGTGGAACC AGCACTATGATCTATATGTTGGGAAAACGGATTCGATAACCATTAGCGTGTGGAACCATA AGAAAATTCACAAGAAACAGGGAGCTGGCTTCCTGGGCTGTGTGCGGCTGCTCTCCAATG CCATCAGCAGATTAAAAGATACCGGATACCAGCGTTTGGATCTATGCAAACTAAACCCCT CAGATACTGATGCAGTTCGTGGCCAGATAGTGGTCAGTTTACAGACACGAGACAGAATAG GAACCGGCGGCTCGGTGGTGGACTGCAGAGGACTGTTAGAAAATGAAGGAACGGTGTATG AAGACTCGGGGCCTGGGAGGCCGCTCAGCTGCTTCATGGAGGAACCAGCCCCTTACACAG ATAGCACCGGTGCTGCTGCTGGAGGAGGGAATTGCAGGTTCGTGGAGTCCCCAAGTCAAG ATCAAAGACTTCAGGCACAGCGGCTTCGAAACCCTGATGTGCGAGGTTCACTACAGACGC CCCAGAACCGACCACACGGCCACCAGTCCCCGGAACTGCCCGAAGGCTACGAACAAAGAA CAACAGTCCAGGGCCAAGTTTACTTTTTGCATACACAGACTGGAGTTAGCACGTGGCACG ACCCCAGGATACCAAGAGACCTTAACAGTGTGAACTGTGATGAACTTGGACCACTGCCGC CAGGCTGGGAAGTCAGAAGTACAGTTTCTGGGAGGATATATTTTGTAGATCATAATAACC GAACAACCCAGTTTACAGACCCAAGGTTACACCACATCATGAATCACCAGTGCCAACTCA AGGAGCCCAGCCAGCCGCTGCCACTGCCCAGTGAGGGCTCTCTGGAGGACGAGGAGCTTC CTGCCCAGAGATACGAAAGAGATCTAGTCCAGAAGCTGAAAGTCCTCAGACACGAACTGT CGCTTCAGCAGCCCCAAGCTGGTCATTGCCGCATCGAAGTGTCCAGAGAAGAAATCTTTG AGGAGTCTTACCGCCAGATAATGAAGATGCGACCGAAAGACTTGAAAAAACGGCTGATGG TGAAATTCCGTGGGGAAGAAGGTTTGGATTACGGTGGTGTGGCCAGGGAGTGGCTTTACT TGCTGTGCCATGAAATGCTGAATCCTTATTACGGGCTCTTCCAGTATTCTACGGACAATA TTTACATGTTGCAAATAAATCCGGATTCTTCAATCAACCCCGACCACTTGTCTTATTTCC ACTTTGTGGGGCGGATCATGGGGCTGGCTGTGTTCCATGGACACTACATCAACGGGGGCT TCACAGTGCCCTTCTACAAGCAGCTGCTGGGGAAGCCCATCCAGCTCTCAGATCTGGAAT CTGTGGACCCAGAGCTGCATAAGAGCTTGGTGTGGATCCTAGAGAACGACATCACGCCTG TACTGGACCACACCTTCTGCGTGGAACACAACGCCTTCGGGCGGATCCTGCAGCATGAAC TGAAACCCAATGGCAGAAATGTGCCAGTCACAGAGGAGAATAAGAAAGAATACGTCCGGT TGTATGTAAACTGGAGGTTTATGAGAGGAATCGAAGCCCAGTTCTTAGCTCTGCAGAAGG GGTTCAATGAGCTCATCCCTCAACATCTGCTGAAGCCTTTTGACCAGAAGGAACTGGAGC TGATCATAGGCGGCCTGGATAAAATAGACTTGAACGACTGGAAGTCGAACACGCGGCTGA AGCACTGTGTGGCCGACAGCAACATCGTGCGGTGGTTCTGGCAAGCGGTGGAGACGTTCG ATGAAGAAAGGAGGGCCAGGCTCCTGCAGTTTGTGACTGGGTCCACGCGAGTCCCGCTCC AAGGCTTCAAGGCTTTGCAAGGTTCTACAGGCGCGGCAGGGCCCCGGCTGTTCACCATCC ACCTGATAGACGCGAACACAGACAACCTTCCGAAGGCCCATACCTGCTTTAACCGGATCG ACATTCCACCATATGAGTCCTATGAGAAGCTCTACGAGAAGCTGCTGACAGCCGTGGAGG AGACCTGCGGGTTTGCTGTGGAGTGAAAAGCAACCAAAGGCAACAGAGTCTAGCTCATGG CCACCAGACCAAAAAGCATCCAGCTTCTGTGCACCTCCTGCAAAGCTGGCAGAGGCCCTG GAATTCCAGATCACCTGAGGGGAAAGGGTTGTCTCTCTCCTTTCTGTTGGGGGAGGGGGA TGGGGGACTTTTGTTGGTGGCTCCCACCCATATATCCCTCCTTTACCATAGTACTCCCAC CCACTTCCATCACCCATCCAATAAAATGCAGCCAGGTTTAGCCTTTGGCTTTGGTCACAC AGGAAAAAAAAAAAAAAAAAACTCGAC >OriGene 5' read for NM_181349 unedited
AGCATTTTGTAATACGACTCACTTATAGGGCGGCCGCGAATTCGCACGAGGCGCTGGATC CGCGGCTGCCCGATCGTTGGCGGGAGATGTCGAACCCCGGGACACGCAGGAACGGCTCCA GCATCAAGATCCGTCTGACAGTGTTATGTGCCAAGAACCTTGCAAAGAAAGACTTCTTCA GGCTCCCTGACCCTTTTGCAAAGATTGTCGTGGATGGGTCTGGGCAGTGCCACTCAACCG ACACTGTGAAAAACACATTGGACCCAAAGTGGAACCAGCACTATGATCTATATGTTGGGA AAACGGATTCGATAACCATTAGCGTGTGGAACCATAAGAAAATTCACAAGAAACAGGGAG CTGGCTTCCTGGGCTGTGTGCGGCTGCTCTCCAATGCCATCAGCAGATTAAAAGATACCG GATACCAGCGTTTGGATCTATGCAAACTAAACCCCTCAGATACTGATGCAGTTCGTGGCC AGATAGTGGTCAGTTTACAGACACGAGACAGAATAGGAACCGGCGGCTCGGTGGTGGACT GCAGAGGACTGTTAGAAAATGAAGGAACGGTGTATGAAGACTCGGGGCCTGGGAGGCCGC TCAGCTGCTTCATGGAGGAACCAGCCCCTTACACAGATAGCACCGGTGCTGCTGCTGGAG GAGGGAATTGCAGGTTCGTGGAGTCCCCAAGTCAAGATCAAAGACTTCAGGCACAGCGGC TTCGAAACCCTGATGTGCGAGGNTCACTACAGACGCCCCAGAACCGACCACACGGNCACC AGTCCNCGGAACTGCCCCGAGGCTACGANACAAGACAACAGTCCAGGGCCAAGTTTACTT TNTGCATACACAGACTGGGAGTAGCACGTGGCACGACCCCCAGATACAAGAGACCTTACA GTGTGAACTGTGATGAACTTGGACCACTGCCGCCAGCCTGGGAAGTCAAAGTACAGTTTC G >OriGene 3' read for NM_181349 unedited
ATGACCGCGGCCGCAATCTANAATCGAGTTTTTTTTTTTTTTTTTTCCTGTGTGACCAAA GCCAAAGGCTAAACCTGGCTGCATTTTATTGGATGGGTGATGGAAGTGGGTGGGAGTACT ATGGTAAAGGAGGGATATATGGGTGGGAGCCACCAACAAAAGTCCCCCATCCCCCTCCCC CAACAGAAAGGAGAGAGACAACCCTTTCCCCTCAGGTGATCTGGAATTCCAGGGCCTCTG CCAGCTTTGCAGGAGGTGCACAGAAGCTGGATGCTTTTTGGTCTGGTGGCCATGAGCTAG ACTCTGTTGCCTTTGGTTGCTTTTCACTCCACAGCAAACCCGCAGGTCTCCTCCACGGCT GTCAGCAGCTTCTCGTAGAGCTTCTCATAGGACTCATATGGTGGAATGTCGATCCGGTTA AAGCAGGTATGGGCCTTCGGAAGGTTGTCTGTGTTCGCGTCTATCAGGTGGATGGTGAAC AGCCGGGGCCCTGCCGCGCCTGTAGAACCTTGCAAAGCCTTGAAGCCTTGGAGCGGGACT CGCGTGGACCCAGTCACAAACTGCAGGAGCCTGGCCCTCCTTTCTTCATCGAACGTCTCC ACCGCTGGCCAGAACCACCGCACGATGTTGCTGTCGGCCACACAGTGCTTCAGCCGCGTG TTCGACTTCCAGTCGTTCAAGTCTATTTTATCCAGGCCCGCCTATGATCAGCTCCCAGTC CCTTTCTGGTCAAAAAGGCTTCACCAGATGTTGAGGGATGAGCTCATTGAACCCCTTCTG CAGAGCTAAGAACTGGGCTTCGATTCCTCTCATAACCTCCAGTTTCATACAACCGAACGT ATTCTTTCTAATCTCCTTTGTGACGGCACATTTCTGCCATTGGGTTCAATTCATGCTGCA GAACCGCCCGAGGCTTGGTGTCCACCCAAAGTTGGGTCCAAACAGGCGGAAGTCTTCTCT AGATCCCCCAATTT |
Restriction Sites | NotI-NotI |
ACCN | NM_181349 |
Insert Size | 2440 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_181349.1, NP_851994.1 |
RefSeq Size | 5659 bp |
RefSeq ORF | 2196 bp |
Locus ID | 57154 |
UniProt ID | Q9HCE7 |
Protein Families | Druggable Genome |
Protein Pathways | Endocytosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis |
Gene Summary | This gene encodes a ubiquitin ligase that is specific for receptor-regulated SMAD proteins in the bone morphogenetic protein (BMP) pathway. This protein plays a key roll in the regulation of cell motility, cell signalling, and cell polarity. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Dec 2010] Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. Isoform 2 is shorter than isoform 1. |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC219536 | SMURF1 (Myc-DDK-tagged)-Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2 |
CNY 5,352.00 |
|
RC219536L1 | Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, Myc-DDK-tagged |
CNY 7,752.00 |
|
RC219536L2 | Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mGFP tagged |
CNY 7,700.00 |
|
RC219536L3 | Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, Myc-DDK-tagged |
CNY 7,752.00 |
|
RC219536L4 | Lenti ORF clone of Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2, mGFP tagged |
CNY 7,752.00 |
|
RG219536 | SMURF1 (tGFP-tagged) - Human SMAD specific E3 ubiquitin protein ligase 1 (SMURF1), transcript variant 2 |
CNY 6,952.00 |