PPP2R3A (NM_181897) Human Untagged Clone
CAT#: SC107434
PPP2R3A (untagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2
CNY 3,944.00
CNY 8,550.00
Product images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Synonyms | PPP2R3; PR72; PR130 |
Vector | pCMV6-XL5 |
E. coli Selection | Ampicillin (100 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
>OriGene ORF within SC107434 sequence for NM_181897 edited (data generated by NextGen Sequencing)
ATGATGATCAAGGAAACATCTCTACGAAGGGACCCGGATTTAAGGGGAGAGCTAGCTTTC CTGGCAAGGGGCTGTGATTTTGTTCTCCCTTCACGGTTTAAGAAGCGGCTGAAGTCATTT CAGCAGACACAGATTCAAAATAAACCAGAAAAGAAACCTGGAACACCACTCCCACCTCCA GCCACCTCTCCAAGTAGTCCCCGACCTCTCTCCCCGGTTCCCCATGTGAATAATGTTGTG AATGCGCCATTGTCCATAAACATTCCACGGTTCTACTTTCCTGAAGGACTCCCAGATACC TGTAGTAATCATGAACAAACTCTAAGCAGAATTGAAACTGCTTTCATGGATATTGAAGAA CAGAAAGCAGACATTTATGAAATGGGGAAAATTGCAAAGGTCTGTGGCTGTCCTCTCTAT TGGAAAGCCCCCATGTTCAGGGCTGCAGGGGGAGAGAAGACAGGATTTGTGACAGCACAG TCATTCATTGCCATGTGGAGAAAGTTGCTGAATAACCATCATGATGATGCCTCTAAATTC ATCTGTCTTCTAGCAAAGCCCAACTGCAGCTCTCTAGAACAGGAGGATTTCATCCCTCTA CTTCAGGATGTGGTGGATACCCACCCTGGTCTCACGTTCCTGAAAGATGCTCCAGAATTC CACTCCCGCTACATCACCACGGTTATTCAGAGAATATTCTACACAGTCAACAGATCTTGG AGTGGAAAAATTACTTCGACAGAGATAAGAAAAAGCAACTTTTTGCAAACCCTAGCACTT TTGGAAGAAGAGGAAGATATAAACCAAATTACAGATTACTTCTCCTATGAACATTTCTAT GTTATTTATTGTAAATTCTGGGAACTAGATACTGATCACGACCTCTACATCAGCCAGGCC GATCTGTCTCGATACAATGACCAGGCTTCATCAAGCAGGATTATTGAAAGGATATTCTCT GGTGCAGTAACAAGGGGAAAAACAATACAGAAAGAGGGAAGAATGAGCTATGCAGATTTT GTTTGGTTTTTGATCTCTGAAGAAGACAAAAGGAATCCTACCAGCATTGAGTATTGGTTC CGCTGCATGGATGTGGATGGAGACGGTGTACTCTCCATGTATGAGCTGGAGTACTTCTAT GAGGAGCAGTGTGAACGGATGGAAGCCATGGGAATTGAGCCCTTGCCATTCCATGATTTA CTGTGCCAGATGCTTGACCTAGTGAAGCCAGCTGTTGATGGCAAAATAACTCTAAGAGAT CTGAAGAGGTGCAGAATGGCTCACATCTTCTATGACACTTTCTTTAATCTGGAGAAATAC TTAGACCATGAACAGAGAGATCCCTTTGCGGTCCAGAAGGATGTTGAGAACGATGGGCCT GAGCCCTCAGACTGGGACCGGTTTGCCGCTGAGGAGTATGAGACGCTTGTTGCAGAGGAA TCTGCCCAAGCACAATTCCAGGAAGGCTTTGAAGATTATGAAACAGATGAACCTGCCTCT CCCTCTGAATTTGGAAACAAAAGCAATAAAATATTAAGTGCAAGCCTTCCAGAGAAATGT GGAAAGCTTCAATCAGTGGATGAAGAATAG Clone variation with respect to NM_181897.2 |
Restriction Sites | NotI-NotI |
ACCN | NM_181897 |
Insert Size | 3870 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Reference Data | |
RefSeq | NM_181897.1, NP_871626.1 |
RefSeq Size | 4664 bp |
RefSeq ORF | 1590 bp |
Locus ID | 5523 |
UniProt ID | Q06190 |
Protein Families | Druggable Genome, Phosphatase |
Gene Summary | This gene encodes one of the regulatory subunits of the protein phosphatase 2. Protein phosphatase 2 (formerly named type 2A) is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2 holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B'' family. The B'' family has been further divided into subfamilies. The product of this gene belongs to the alpha subfamily of regulatory subunit B''. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Jun 2010] Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region, compared to variant 1. The resulting isoform (2), also known as isoform alpha or isoform PR72, contains a distinct N-terminus, compared to isoform 1. |
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Product Manuals |
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Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC215798 | PPP2R3A (Myc-DDK-tagged)-Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2 |
CNY 3,936.00 |
|
RC215798L1 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2, Myc-DDK-tagged |
CNY 6,336.00 |
|
RC215798L2 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2, mGFP tagged |
CNY 6,560.00 |
|
RC215798L3 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2, Myc-DDK-tagged |
CNY 6,560.00 |
|
RC215798L4 | Lenti ORF clone of Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2, mGFP tagged |
CNY 6,560.00 |
|
RG215798 | PPP2R3A (tGFP-tagged) - Human protein phosphatase 2, regulatory subunit B'', alpha (PPP2R3A), transcript variant 2 |
CNY 5,536.00 |
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