B3GAT2 (AK055248) Human Untagged Clone

Specifications

Product Data
Type	Human Untagged Clone
Tag	Tag Free
Synonyms	GLCATS
Vector	pCMV6-XL4
E. coli Selection	Ampicillin (100 ug/mL)
Mammalian Cell Selection	None
Sequence Data	Fully Sequenced ORF (showhide) >OriGene ORF within SC103872 sequence for AK055248 edited (data generated by NextGen Sequencing) ATGCGAACCACCCGCAAGGTCTCCGTCTGGCCTGTGGGCCTGGTTGGTGGGCGGCGCTAC GAACGTCCGCTGGTGGAAAACGGCAAAGTTGTTGGCTGGTACACCGGCTGGAGAGCAGAC AGGCCTTTTGCCATCGACATGGCAGGATTTGCTGTAAGTCTTCAAGTCATTTTGTCCAAT CCAAAAGCTGTATTTAAGCGTCGTGGATCCCAGCCAGGGATGCAAGAATCTGACTTTCTC AAACAGATAACAACAGTCGAAGAACTGGAACCGAAAGCAAATAACTGCACTAAGGTTCTC GTGTGGCACACTCGGACAGAGAAGGTTAATCTAGCCAACGAGCCAAAGTACCACCTGGAC ACAGTGAAAATTGAGGTATAA Clone variation with respect to AK055248.1 5' Read Nucleotide Sequence (showhide) >OriGene 5' read for AK055248 unedited GGGTGCATATATAGNATACGACTCATATAGGCGGCCGCGAAATTCGCACGAGGGGGCGCG GGGTGAGCGGGGGCACTGGCCTGCGCGTGTCCACGTCGAGCATGATGATGACAATTAGGA TCCAGGGCAGGAGGATAAAGAAGCGGGTGAAAAGCGCGGACTTCATGGTGCACGCTCCCT GGCCTCTCGGACACCCCAGAGAGGGGCGAGCCGAGGGACCCCAGTGCGCAGCTTGGACAG CGGCGGCGCCAGCACTTAGGGAGTGGTGATGGGTGCGCTGTCGACGACAACACCTATAGT CTGGAGCTCTTCCAGGAGATGCGAACCACCCGCAAGGTCTCCGTCTGGCCTGTGGGCCTG GTTGGTGGGCGGCGCTACGAACGTCCGCTGGTGGAAAACGGCAAAGTTGTTGGCTGGTAC ACCGGCTGGAGAGCAGACAGGCCTTTTGCCATCGACATGGCAGGATTTGCTGTAAGTCTT CAAGTCATTTTGTCCAATCCAAAAGCTGTATTTAAGCGTCGTGGATCCCAGCCAGGGATG CAAGAATCTGACTTTCTCAAACAGATAACAACAGTCGAAGAACTGGAACCGAAAGCAAAT AACTGCACTAAGGTTCTCGTGTGGCACACTCGGACAGAGAAGGTTAATCTAGCCAACGAG CCAAAGTACCACCCTGGACACAGTGAAAATTTGAGTATAAATTGAAGCAGCAACTGGTGC AGTTTGTCCAGCCAGTGGATCCATATGGGAGAAGATGTTTGCAGATTAAGCCACCAAGCA TTCAAGGATTGGTTGGTTTACTTTAATACAGCAGCCCTTCCTTGTCCATCTGAGGACATC CTGTCTTAAAAGGAGCTTGTCCAGTAAACAAAACCTAATTGGGAGGTTGGTACACAAAGG ATAGTGTTGGCTTAATTTGTCCTGCCAGGTTTTCTTACAAN 3' Read Nucleotide Sequence (showhide) >OriGene 3' read for AK055248 unedited GAACCGCGGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATTTAGGAAGTATA TAAGATTTTAATAGAGAATAATATCTATCATACAAAGTTTATAGGATAATTTTAGACTAT TTTACTTCCTAAAATTTTCTTACTCCTTAGTAGTTAAAGCACAATATCCTAGTGTAGGTG AAAGTTAGTTAGAGTAGCGGACTCATTAAAATCCCAAGATTGCTTTGGTATTTTTTTTTA AGTAAGTTGTGGTTAATCTTTGGGGAAACTGTATCTAGAAAGTAGATAAAGAAGGGAACA AAGTAGCTATTTGCTTTAAGAAATATTTGTATGGTACTCTGTTTTTATTCCAGTGCTTGG ACTAGTTGTCACATTAATGAAAAAATGACCAACTGTGTGGCTAAAGAAACAAGAATTAAA AGTGAAGTAAGCCTCTTGAACTAAGCCTTTTATATGTTTCACAGATGAATCAGCAGATGG CTGGCATGAGTATCAGTAGTGCAACCCCTACTGCAGGTTTTGGCCAGCCCTCCAGCACAA CAGCAGGATGGTCTGGAAGCTCATCAGGTCAGACTCTCAGCACACAACTGTGGAAATGAA AACTGCAATACAAGTTTCATCCAGAACTACCACCTGACATTCCTTGCTGNAACGCATCTA GTTCCCCTGTTTATTCATATGCATATTTTTTTTCTTTTTACCCATTTGTTCATATTAAGA ATGATCTGATTGACCGTGTTGGTCTGTACTGATTCAATTGATGTGGTGAAAAACAAGTTG ATAATCCTTTTATGTCAAGGCAGCTNTGCTCATATTTCCATGATNTCTGTACTGCATATT TGGGAAGCTGCTCACTGGCAAATCAGTTTCCTCTCATAAAATATTGCCTCATGTTTGGCT ATAGGAAAGAGTTTCATGTAGTAAAACCCCTAAAGGTAAACCCACCCCCAATACCCGTAT CCTGTGGAGGTCCTGGTGACAA
Restriction Sites	NotI-NotI
ACCN	AK055248
Insert Size	2600 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	AK055248.1, BAB70889.1
RefSeq Size	2596 bp
RefSeq ORF	381 bp
Locus ID	135152
Domains	Glyco_transf_43
Protein Families	Transmembrane
Protein Pathways	Chondroitin sulfate biosynthesis, Heparan sulfate biosynthesis, Metabolic pathways
Gene Summary	The product of this gene is a transmembrane protein belonging to the glucuronyltransferase family, and catalyzes the transfer of a beta-1,3 linked glucuronic acid to a terminal galactose in different glycoproteins or glycolipids containing a Gal-beta-1-4GlcNAc or Gal-beta-1-3GlcNAc residue. The encoded protein is involved in the synthesis of the human natural killer-1 (HNK-1) carbohydrate epitope, a sulfated trisaccharide implicated in cellular migration and adhesion in the nervous system. [provided by RefSeq, Jul 2008]

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