A2m (BC072642) Mouse Tagged ORF Clone

CAT#: MG212004

  • TrueORF®

A2m (tGFP-tagged) - Mouse alpha-2-macroglobulin (cDNA clone MGC:95993 IMAGE:6839761)



  "BC072642" in other vectors (1)

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Mouse Protein / Lysate Request

CNY 14,250.00


货期*
5周

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-AC-GFP, mammalian vector with C-terminal tGFP tag, 10ug
    • 10 ug

CNY 6,080.00


Mouse monoclonal turboGFP antibody, clone OTI2H8
    • 100 ul

CNY 1,000.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


A2M Antibody - middle region
    • 50 ug

CNY 4,628.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag TurboGFP
Synonyms A2mp
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MG212004 representing BC072642
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGGGAAGCGCTGGCTCCCAAGCCTGGCCCTGCTGCCGCTGCCGCCACCGCTGCTGCTGCTGCTGTTGC
TGCTGCTGCCCACAAATGCCTCAGCACCACAGAAACCAATCTACATGGTGATGGTTCCCTCCCTGCTCCA
CGCTGGCACCCCTGAGAAGGGCTGCCTCCTGTTCAACCACCTAAACGAGACAGTAACAGTGAAAGTATCC
ATGGAGTCTGTCCGTGGGAACCAAAGCCTCTTCACTGACCTTGTTGTTGACAAGGACTTATTCCACTGTG
CCTCCTTCATCGTCCCACAGTCTTCATCCAATGAGGTGATGTTTCTCACTGTCCAAGTAAAAGGACCAAC
TCATGAGTTCAGGAGGAGGAGCACGGTGCTGATCAAGACTAAAGAGAGCCTGGTCTTTGCTCAGACTGAC
AAGCCCATCTACAAACCAGGACAGATGGTGAGATTTCGTGTTGTCTCATTGGACGAAAATTTCCACCCCC
TTAATGAATTGATTCCTCTACTGTACATTCAGGATTCAAAAAAAAATCGCATTGCACAGTGGCAGAATTT
CCGCTTAGAGGGTGGCCTCAAACAGCTGTCCTTCCCCCTCTCCTCAGAGCCCACTCAGGGCTCCTACAAG
GTGGTGATACGTACAGAATCAGGCAGGACCGTCGAGCACCCCTTCTCTGTGAAGGAATTTGTGCTTCCCA
AGTTTGAAGTCAAAGTTGCAGTACCAGAGACAATCACCATCCTGGAGGAAGAGATGAATGTGTCCGTGTG
TGGCATATACACCTATGGGAAGCCTGTTCCAGGACACGTGACTGTGAACATTTGCAGAAAGTACAGTAAT
CCTTCTAGCTGCTTTGGTGAAGAATCGCTGGCTTTCTGTGAGAAATTCAGTCAACAGCTAGATGGCCGTG
GCTGCTTCTCACAGCTAGTGAAAACCAAGTCTTTCCAGCTAAAGAGGCAAGAGTATGAGATGCAGCTTGA
TGTGAATGCCAAGATCCAAGAAGAAGGAACAGGTGTGGAAGAAACCGGTAAAGGGCTCACTAAGATCACA
AGAACTATAACAAAACTATCATTTGTGAACGTGGATACACATTTCAGACAAGGAATTCCTTTCGTTGGGC
AGGTGCTCCTGGTGGATGGGAGAGGCACCCCTATTCCATATGAAATGATCTTCATTGGAGCTGATGAAGC
AAACCAGAACATAAATACTACCACTGATAAGAATGGCCTGGCACGGTTCTCCATCAACACTGATGACATC
ATGGGCACGTCCCTAACTGTCAGGGCCAAATACAAAGATAGCAATGTCTGCTATGGATTTAGATGGTTGA
CAGAAGAGAACGTAGAGGCTTGGCACACTGCCAACGCTGTCTTCTCACCGAGCAGAAGCTTCGTGCACCT
GGAATCCCTGCCCTATAAACTGCGCTGTGAGCAAACCCTGGCGGTCCAGGCTCATTATATTCTAAATGAC
GAGGCTGTGCTGGAGCGGAAGGAGTTGGTCTTCTATTATCTGATGATGGCAAAGGGAGGCATTGTTCGGG
CAGGGACTCACGTTCTGCCTGTGACGCAGGGACACAAGAAAGGCCATTTCTCCATCCTCATCTCGATGGA
GACAGACCTGGCTCCTGTAGCTCGACTGGTCCTTTATACCATTCTGCCCAATGGAGAAGTGGTTGGAGAT
ACTGTTAAATATGAGATTGAAAAGTGCCTGGCCAACAAGGTGGATTTGGTCTTCCACCCAAATATCGGTC
TTCCGGCCACACGTGCCTTCCTCAGTGTCATGGCTTCTCCTCAGTCTCTCTGTGGCCTGCGAGCTGTGGA
CCAAAGCGTACTGCTCACAAAGCCTGAGGCCGAACTCTCTGCATCCCTGGTTTATGATCTGCTACCAGTG
AAAGACCTCACTGGCTTCCCTAAGGGTGTGAATCAACAGGAAGAAGACACTAATGGCTGCCTTAAGCAAA
ATGACACTTACATTAATGGAATCCTGTACTCCCCAGTACAGAATACAAATGAAGAGGACATGTATGGCTT
CCTAAAGGATATGGGCTTAAAGGTATTCACCAACTTGAACATCCGTAAACCCAAAGTCTGTGAACGCCTT
GGAGTCAATAAAATACCAGCTGCATACCACCTTGTAAGCCAAGGCCACATGGATGCTTTTCTAGAGTCTT
CAGAGTCTCCCACAGAGACTACACGAAGCTACTTCCCTGAGACGTGGATCTGGGACTTGGTGATCGTGGA
CTCAACAGGAGTGGCTGAAATGGAAGTGACAGTCCCTGACACCATCACTGAATGGAAGGCTGGGGCCTTC
TGCCTGTCCAATGACACTGGGTTGGGCCTCTCTCCTGTGATCGATTTCCAAGCCTTCCAGCCCTTCTTCG
TGGATCTCACAATGGCCTACTCCGTGATCCGTGGAGAGGCCTTCACGCTCAAGGCCACTGTGCTGAACTA
CCTCCAGACATGCATCCGGGTTGGTGTACAGCTGGAGGCCTCTCCTGATTTCCTAGCTACCCCAGAGGAG
AAGGAACAAAAGTCTCACTGCATCTGTATGAACGAGCGGCATACCATGTCCTGGGCGGTGATCCCCAAGT
CATTAGGAAATGTGAATTTCACAGTGAGTGCAGAGGCACTGGACTCTAAGGAGCTGTGTAGGAATGAGGT
GCCAGTGGTTCCTGAACGTGGAAAGAAAGACACGATCATCAAGTCCCTGCTGGTTGAACCTGAAGGTCTA
GAGAATGAAGTGACATTCAACAGTTTGCTTTGTCCAACAGGTGCTGAGGTATCGGAACAGATATCTCTGA
AACTGCCATCAGATGTGGTAGAGGAATCTGCCCGAGCTTCTGTCACAGTTTTGGGAGATATATTGGGTTC
TGCTATGCAGAACACACAGGATCTCCTTAAGATGCCCTATGGCTGTGGAGAACAGAACATGGTTCTGTTT
GCTCCTAATATCTATGTCCTGGACTATCTGAATGAAACAGAGCAGCTGACACAGGAGATCAAGACCAAGG
CCATTACCTATCTCAATACGGGTTACCAAAGACAATTAAACTACAAGCACCGGGATGGCTCCTACAGTAC
TTTTGGGGATAAACCTGGTAGGAGTCATGCCAATACCTGGCTCACAGCCTTTGTACTGAAGAGTTTTGCT
CAGGCTCGAAGATATATCTTCATCGATGAATCACACATCACCCAAGCCCTCACCTGGCTCTCCCAGCAGC
AGAAGGACAATGGCTGCTTCAGGAGCTCCGGGTCACTGCTCAACAATGCCATGAAGGGAGGAGTAGAAGA
TGAAGTCACTTTGTCTGCCTACATCACCATTGCTCTCCTGGAGATGTCTCTTCCTGTCACTCATCCTGTT
GTCCGAAATGCCCTCTTTTGCCTGGACACAGCCTGGAAGTCAGCGAGGAGAGGAGCCAGCGGCAACCATG
TCTACACTAAGGCACTGTTGGCCTATGCATTTGCCCTTGCTGGTAACCAGGACACGAAGAAGGAGATACT
GAAATCACTCGATGAGGAGGCGGTAAAAGAAGACAATTCTGTCCACTGGACCAGAGCTCAGAAACCCAGA
GTGCCAGCAGATCTCTGGTACCAACCCCAGGCTCCCTCGGCTGAGGTAGAGATGACCGCTTATGTGCTCC
TGGCTTATCTTACCACTGAGCTGGTTCCAACCCGAGAGGACCTGACTGCTGCAATGCTCATTGTGAAGTG
GCTCACAAAGCAGCAGAATTCCCACGGTGGCTTCTCCTCCACCCAGGACACGGTAGTGGCTCTCCATGCT
TTGTCCAAATACGGAGCAGCTACTTTCACAAGAGCGAAGAAAGCTGCACATGTGACCATCCAGTCTTCTG
GCGCATTTTATACAAAATTCCAAGTGAACAACGACAACCAGTTATTATTACAGAGAGTCACATTGCCAAC
TGTGCCTGGGGATTACACCGCAAAGGTAGCTGGAGAAGGGTGTGTCTACCTCCAGACATCCTTGAAATAT
AGTGTTCTCCCGAGGGAGAAGGAGTTTCCATTTGCTTTGGTGGTGCAGACTCTGCCTGGGACTTGTGAGG
ATCTCAAAGCTCACACCACCTTCCAGATCTCACTCAATATCAGTTACATTGGAAGCCGTTCTGACTCCAA
CATGGCAATTGCTGATGTGAAGATGGTGTCTGGCTTCATCCCCTTGAAACCAACAGTGAAAATGCTTGAA
AGATCTGTGCATGTGAGCCGGACGGAAGTCAGCAATAACCATGTCTTGATTTACTTGGATAAGGTGTCAA
ATCAGATGCTGACCTTGTTCTTCATGGTGCAGCAAGATATTCCAGTGAGAGACCTGAAGCCAGCCATAGT
GAAAGTCTATGATTACTACGAGAAAGATGAGTTTGCAGTTGCAAAATACAGTGCTCCCTGCAGTGCAGGT
TATGGAAATGCC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>MG212004 representing BC072642
Red=Cloning site Green=Tags(s)

MGKRWLPSLALLPLPPPLLLLLLLLLPTNASAPQKPIYMVMVPSLLHAGTPEKGCLLFNHLNETVTVKVS
MESVRGNQSLFTDLVVDKDLFHCASFIVPQSSSNEVMFLTVQVKGPTHEFRRRSTVLIKTKESLVFAQTD
KPIYKPGQMVRFRVVSLDENFHPLNELIPLLYIQDSKKNRIAQWQNFRLEGGLKQLSFPLSSEPTQGSYK
VVIRTESGRTVEHPFSVKEFVLPKFEVKVAVPETITILEEEMNVSVCGIYTYGKPVPGHVTVNICRKYSN
PSSCFGEESLAFCEKFSQQLDGRGCFSQLVKTKSFQLKRQEYEMQLDVNAKIQEEGTGVEETGKGLTKIT
RTITKLSFVNVDTHFRQGIPFVGQVLLVDGRGTPIPYEMIFIGADEANQNINTTTDKNGLARFSINTDDI
MGTSLTVRAKYKDSNVCYGFRWLTEENVEAWHTANAVFSPSRSFVHLESLPYKLRCEQTLAVQAHYILND
EAVLERKELVFYYLMMAKGGIVRAGTHVLPVTQGHKKGHFSILISMETDLAPVARLVLYTILPNGEVVGD
TVKYEIEKCLANKVDLVFHPNIGLPATRAFLSVMASPQSLCGLRAVDQSVLLTKPEAELSASLVYDLLPV
KDLTGFPKGVNQQEEDTNGCLKQNDTYINGILYSPVQNTNEEDMYGFLKDMGLKVFTNLNIRKPKVCERL
GVNKIPAAYHLVSQGHMDAFLESSESPTETTRSYFPETWIWDLVIVDSTGVAEMEVTVPDTITEWKAGAF
CLSNDTGLGLSPVIDFQAFQPFFVDLTMAYSVIRGEAFTLKATVLNYLQTCIRVGVQLEASPDFLATPEE
KEQKSHCICMNERHTMSWAVIPKSLGNVNFTVSAEALDSKELCRNEVPVVPERGKKDTIIKSLLVEPEGL
ENEVTFNSLLCPTGAEVSEQISLKLPSDVVEESARASVTVLGDILGSAMQNTQDLLKMPYGCGEQNMVLF
APNIYVLDYLNETEQLTQEIKTKAITYLNTGYQRQLNYKHRDGSYSTFGDKPGRSHANTWLTAFVLKSFA
QARRYIFIDESHITQALTWLSQQQKDNGCFRSSGSLLNNAMKGGVEDEVTLSAYITIALLEMSLPVTHPV
VRNALFCLDTAWKSARRGASGNHVYTKALLAYAFALAGNQDTKKEILKSLDEEAVKEDNSVHWTRAQKPR
VPADLWYQPQAPSAEVEMTAYVLLAYLTTELVPTREDLTAAMLIVKWLTKQQNSHGGFSSTQDTVVALHA
LSKYGAATFTRAKKAAHVTIQSSGAFYTKFQVNNDNQLLLQRVTLPTVPGDYTAKVAGEGCVYLQTSLKY
SVLPREKEFPFALVVQTLPGTCEDLKAHTTFQISLNISYIGSRSDSNMAIADVKMVSGFIPLKPTVKMLE
RSVHVSRTEVSNNHVLIYLDKVSNQMLTLFFMVQQDIPVRDLKPAIVKVYDYYEKDEFAVAKYSAPCSAG
YGNA

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN BC072642
ORF Size 4424 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq BC072642, AAH72642
RefSeq Size 4615 bp
RefSeq ORF 4424 bp
Locus ID 232345
Gene Summary Is able to inhibit all four classes of proteinases by a unique 'trapping' mechanism. This protein has a peptide stretch, called the 'bait region' which contains specific cleavage sites for different proteinases. When a proteinase cleaves the bait region, a conformational change is induced in the protein which traps the proteinase. The entrapped enzyme remains active against low molecular weight substrates (activity against high molecular weight substrates is greatly reduced). Following cleavage in the bait region a thioester bond is hydrolyzed and mediates the covalent binding of the protein to the proteinase (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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