Ank1 (NM_001277280) Mouse Untagged Clone

Specifications

Product Data
Type	Mouse Untagged Clone
Tag	Tag Free
Synonyms	Ank-1; nb; pale
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	Fully Sequenced ORF (showhide) >MC229660 representing NM_001277280 Red=Cloning site Blue=ORF Orange=Stop codon TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGGCGGCCAAACAGCTGAAGAAAATCAAAGACATCGAGGCGCAGGCCCTCCAGGAGCAGAAGG AGAAGGAGGAATCCAACAGGAAGCGGCGGAACCGCTCCCGTGACCGCAAGAAGAAGGCCGATGCTGCTAC CAGCTTTCTGCGGGCGGCACGCTCGGGGAACCTGGACAAGGCTCTGGATCACCTGCGCAATGGAGTGGAC ATTAACACCTGTAACCAGAACGGGTTGAACGGCCTGCATCTGGCCTCCAAAGAAGGCCATGTGAAGATGG TGGTTGAACTTCTGCACAAAGAGATCATTCTAGAAACGACAACCAAGAAGGGGAACACTGCTCTGCACAT CGCTGCCCTTGCTGGTCAGGATGAGGTGGTCCGGGAGCTGGTCAACTATGGAGCCAATGTCAATGCCCAG TCTCAGAAAGGCTTTACTCCCCTGTACATGGCTGCTCAGGAGAACCACTTGGAAGTGGTGAAATTTCTAC TGGAGAATGGAGCCAATCAGAATGTAGCCACAGAAGATGGCTTCACCCCACTGGCCGTGGCTCTACAGCA GGGTCACGAGAATGTGGTGGCTCACCTCATCAACTATGGGACGAAAGGGAAAGTGCGCCTCCCTGCCCTG CACATCGCGGCCCGCAACGATGACACACGGACAGCCGCAGTCCTTCTGCAGAATGACCCCAACCCAGATG TGCTTTCCAAGACGGGATTCACACCCCTCCACATCGCAGCTCACTATGAGAACCTCAACGTGGCCCAGTT GCTCCTCAACAGGGGAGCCAGCGTCAACTTCACACCTCAGAATGGCATCACCCCACTACACATCGCCTCC CGCAGGGGGAACGTGATCATGGTGAGACTCCTGCTGGACCGAGGGGCTCAGATAGAAACGAGGACCAAGG ATGAATTGACACCGCTCCACTGTGCAGCTCGGAATGGACACGTGAGAATCTCAGAGATCCTGCTGGACCA CGGGGCACCCATCCAAGCCAAAACCAAGAATGGCTTGTCCCCAATCCACATGGCCGCTCAGGGAGACCAC CTCGACTGTGTCCGACTTCTATTGCAATACAATGCAGAGATAGACGACATCACCTTGGATCACCTGACTC CTCTCCATGTGGCAGCCCACTGTGGCCACCACCGGGTGGCTAAGGTTCTTTTGGATAAAGGGGCCAAGCC CAACTCCAGAGCCCTGAATGGTTTTACCCCGTTACACATCGCCTGCAAGAAGAACCACATCCGTGTAATG GAGTTGCTGCTGAAGACAGGAGCCTCCATCGACGCGGTCACTGAGTCTGGCCTGACACCTCTCCACGTAG CCTCCTTCATGGGACACCTTCCTATTGTGAAGAACTTACTGCAGCGGGGAGCGTCACCCAATGTCTCCAA TGTGAAAGTAGAAACCCCCTTGCACATGGCAGCCCGAGCAGGGCATACAGAAGTGGCCAAATATTTGCTC CAGAACAAAGCCAAAGCCAACGCCAAGGCCAAGGATGACCAGACACCGCTTCACTGTGCTGCTCGAATCG GCCACACAGGCATGGTGAAGCTCCTGCTGGAGAATGGTGCCAGCCCCAATCTGGCTACCACTGCTGGCCA CACACCCCTACACACCGCAGCCCGTGAGGGACACGTGGACACAGCCCTGGCCCTGCTGGAGAAGGAGGCA TCCCAAGCCTGCATGACCAAGAAAGGATTTACCCCTCTGCACGTGGCTGCTAAGTACGGGAAGGTACGGT TGGCGGAGCTGCTGCTGGAACACGATGCACACCCCAATGCAGCTGGGAAGAACGGCTTGACTCCTCTGCA TGTGGCCGTCCATCACAACAACCTGGACATTGTCAAACTTCTTCTTCCCCGAGGTGGCTCCCCCCACAGC CCTGCCTGGAATGGCTACACTCCTTTGCACATCGCTGCCAAGCAGAACCAGATAGAGGTGGCCCGCAGTC TACTGCAGTACGGAGGGTCAGCGAATGCAGAGTCGGTACAAGGCGTGACCCCACTTCACCTGGCTGCCCA AGAGGGCCACACAGAAATGGTTGCTCTTCTCCTGTCCAAGCAAGCCAACGGCAACCTGGGGAACAAGAGT GGACTCACTCCCCTCCACCTGGTGTCACAAGAAGGCCATGTTCCAGTGGCAGACGTGCTGATCAAACATG GCGTCACCGTGGATGCTACCACCCGGATGGGTTACACCCCGCTCCATGTGGCCAGTCATTATGGAAACAT CAAGCTGGTGAAGTTCTTGCTACAGCACCAGGCAGATGTCAATGCCAAGACCAAGCTAGGATACAGCCCC TTGCACCAGGCAGCTCAACAAGGACACACAGACATTGTGACATTGCTCCTGAAGAATGGTGCTTCTCCAA ATGAGGTCAGCTCGAACGGAACCACACCTCTGGCAATAGCCAAACGTTTGGGCTACATCTCTGTAACAGA CGTGCTCAAGGTGGTCACAGATGAAACCAGTGTGGTGTTAGTCAGTGACAAACACCGGATGAGCTACCCT GAGACGGTGGACGAGATCCTGGACGTGTCTGAAGATGAAGGTGACGAACTTGTCGGCTCGAAGGCTGAAA GGCGGGACTCCCGGGATGTGGGTGAGGAGAAAGAGCTGTTGGATTTTGTGCCGAAGCTAGACCAAGTGGT GGAGTCTCCAGCCATCCCAAGGATCCCTTGTGTCACACCTGAGACCGTTGTGATCCGATCTGAAGATCAG GAACAAGCATCTAAAGAATATGATGAGGATTCCCTCATTCCCAGCAGCCCGGCCACAGAGACCTCAGACA ACATTAGCCCTGTGGCTAGCCCGGTCCACACAGGGTTCCTGGTGAGCTTCATGGTGGATGCCCGAGGGGG ATCCATGAGAGGAAGCCGTCATAATGGCCTCAGGGTGGTGATCCCTCCCCGGACATGCGCAGCACCCACT CGCATCACCTGCCGCCTCGTTAAGCCGCAGAAGCTGAACACGCCACCCCCACTGGCTGAGGAAGAGGGCC TAGCCAGCAGGATCATTGCCCTGGGACCCACGGGGGCCCAGTTCCTTAGCCCTGTGATCGTGGAAATCCC CCATTTTGCCTCCCATGGCCGTGGGGACCGTGAGCTGGTGGTTCTGAGGAGTGAAAACGGCTCTGTGTGG AAGGAGCACAAGAGTCGTTATGGGGAAAGCTACCTGGACCAGATCCTTAATGGCATGGATGAAGAGCTGG GGAGCCTGGAGGAGCTGGAGAAGAAACGTGTGTGCCGCATCATCACCACCGACTTCCCTCTGTACTTTGT CATCATGTCCCGGCTCTGCCAGGACTACGACACCATCGGTCCTGAAGGGGGTTCCCTGAGAAGCAAGCTG GTACCGCTGGTACAGGCAACGTTCCCTGAAAACGCTGTGACCAAGAAAGTGAAGCTGGCTCTGCAGGCCC AGCCTGTCCCGGATGAGCTGGTGACCAAGCTCCTGGGTAACCAGGCCACATTCAGCCCCATTGTGACCGT GGAGCCGAGGCGTCGGAAGTTCCACCGTCCCATCGGGCTGCGAATCCCGCTCCCTCCCTCATGGACGGAC AACCCCAGGGACAGTGGGGAGGGAGACACCACCAGCCTGCGCCTACTGTGTAGCGTCATCGGTGGAACTG ACCAAGCCCAGTGGGAAGATATAACGGGAACAACCAAACTTATATACGCCAATGAGTGTGCCAACTTTAC CACCAATGTCTCAGCCAGGTTCTGGCTGTCAGACTGTCCCCGGACTGCGGAGGCTGTGCACTTTGCCACT CTCCTGTACAAAGAGCTCACAGCAGTCCCCTACATGGCTAAGTTTGTCATTTTTGCCAAGATGAACGACG CTCGGGAAGGACGGCTCCGTTGCTACTGCATGACGGATGACAAAGTGGACAAGACCCTGGAGCAGCATGA AAACTTCGTGGAGGTGGCCCGGAGCAGGGACATAGAGGTTCTGGAAGGGATGCCTCTGTTTGCAGAACTC TCTGGGAACTTGGTTCCTGTCAAGAAAGCGGCCCAACAGCGGAGCTTCCATTTCCAGTCGTTTCGAGAGA ATCGTCTGGCCATCCCCGTGAAGGTGAGGGACAGCAGCCGCGAGCCAGGAGGGTTCTTGTCGTTCCTACG AAAGACAATGAAGTACGAGGACACACAGCACATCCTCTGTCACCTGAATATCACCATGCCCCCCTGCACC AAGGGCAGTGGAGCAGAAGACAGGAGAAGGACCCTGACACCCCTGACCCTTCGATACAGCATTCTCAGCG AGTCGCGGCTGGGTTTTACCAGTGACACAGACCGTGTCGAAATGAGGATGGCTGTCATCAGAGAACACCT CGGCCTAAGCTGGGCAGAGCTGGCCCGGGAGCTGCAGTTCAGCGTGGAAGACATCAACCGGATCCGTGTA GAAAACCCTAACTCCTTGTTGGACCAGAGTACAGCCTTGCTGACCCTCTGGGTGGACCGTGAAGGCGAAA ATGCAAAGATGGAGAATTTGTACACAGCCCTGCGGAACATCGACAGGAGTGAGATTGTTAACATGTTGGA GGGCTCCGGCAGGCAGAGCAGAAACCTCAAACCAGAGCGGAGACATGGGGACCGGGAGTACTCATTGTCA CCCTCCCAGGTGAATGGCCAGCAGCGAGTTCACGCCCGAATCACAGACTCACCCTCAGTGAGGCAGGTGC TGGACAGAAGCCAGGCCAGAACACTGGACTGGGATAAACAGGGTTCCACAGCGGTACACCCGCAAGAAGC CACACAGAGCTCCTGGCAAGAGGAGGTCACGCAGGGCCCACACTCATTCCAGAGAAGGATCACCACCATC CAAGGGCCGGAGCCTGGTGCGCTTCAGGAATACGAGCAGGTGCTGGTGTCTACCAGGGAGCATGTGCAGA GGGGGCCACCTGAGACCGGCAGCCCCAAAGCTGGCAAGGAACCTAGCCTGTGGGCACCTGAGAGCGCCTT CTCTCAAGAGGTGCAGGGGGATGAGCTTCAGAATATTCCAGGAGAGCAGGTGACGGAGGAACAATTCACA GATGAACAGGGCAACATTGTTACCAAGAAGATCATTCGCAAAGTCGTCCGGCAGGTAGACTCGTCTGGTG CCATCGACACCCAGCAGCACGAGGAGGTGGAGCTAAGAGGGAGTGGACTCCAGCCGGACCTGATAGAGGG CAGGAAGGGGGCTCAGATAGTGAAGCGGGCCAGCCTGAAAAGGGGCAAGCAGTGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Restriction Sites	SgfI-MluI
ACCN	NM_001277280
Insert Size	5235 bp
OTI Disclaimer	Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation	Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note	Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq	NM_001277280.2, NP_001264209.1
RefSeq Size	7794 bp
RefSeq ORF	5235 bp
Locus ID	11733
UniProt ID	Q02357
Gene Summary	Attaches integral membrane proteins to cytoskeletal elements; binds to the erythrocyte membrane protein band 4.2, to Na-K ATPase, to the lymphocyte membrane protein GP85, and to the cytoskeletal proteins fodrin, tubulin, vimentin and desmin. Erythrocyte ankyrins also link spectrin (beta chain) to the cytoplasmic domain of the erythrocytes anion exchange protein; they retain most or all of these binding functions. In skeletal muscle, isoform Mu7 together with obscurin may provide a molecular link between the sarcoplasmic reticulum and myofibrils.[UniProtKB/Swiss-Prot Function] Transcript Variant: This variant (3) lacks an alternate in-frame exon and uses an alternate in-frame splice junction in a 3' exon compared to variant 1. The resulting isoform (3) has the same N- and C-termini but lacks two alternate internal segments compared to isoform 1.

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